“…Common human respiratory tract viruses have been summarized for the period from 2009 to 2016, including Rhinoviruses, Paramyxoviruses, Orthomyxoviruses, Coronaviruses, Adenoviruses, Parvoviruses, Herpesviruses, Anelloviruses, Papillomaviruses, and Polyomaviruses (Wylie, 2017). To date, only one study characterized respiratory virome in the areas of COPD in 63 patients with acute COPD exacerbation from Norway, in which an obtained median of 11 million sequence reads per sample contained ∼93% of human reads, 3% of bacterial, 0.1% of viral, and 3% of unknown reads, as identified using NGS without any viral enrichment steps (van Rijn et al, 2019). In this study, we aimed to optimize the pretreatment method to enrich viral particles for NGS sequencing to investigate the virome in pharyngeal clinical samples from hospitalized patients with acute COPD exacerbation.…”