The response of antioxidant systems in Nostoc sphaeroides against UV-B radiation and the protective effects of exogenous antioxidants

Wang, Gaohong; Hu, Chunxiang; Li, Dunhai; Zhang, Delu; Li, Xiaoyan; Chen, Kun; Liu, Yongding

doi:10.1016/j.asr.2007.03.022

Cited by 36 publications

(18 citation statements)

References 50 publications

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“…SOD activity was determined using the method previously described by Wang et al (2007). Briefly, algal cultures treated with UV-B radiation and chemicals were centrifuged at 12,000g for 15 min, and the pellets were then ground to a powder in liquid nitrogen and homogenized in ice-cold 0.1 M sodium phosphate buffer (pH 6.8).…”

Section: Determination Of Sod Activity Mda and Protein Contentsmentioning

confidence: 99%

The combined effects of UV-B radiation and herbicides on photosynthesis, antioxidant enzymes and DNA damage in two bloom-forming cyanobacteria

Chen

Xie

et al. 2012

Ecotoxicology and Environmental Safety

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Section: Determination Of Sod Activity Mda and Protein Contentsmentioning

confidence: 99%

The combined effects of UV-B radiation and herbicides on photosynthesis, antioxidant enzymes and DNA damage in two bloom-forming cyanobacteria

Chen

Xie

et al. 2012

Ecotoxicology and Environmental Safety

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“…Since these microalgae are single cell autotroph, they need sunlight for their growth and have to be exposed to elevated levels of UV radiation in their natural habitat, and simultaneously they have developed mechanisms counteracting the damage effects of UV-B during long term of acclimation. It has been shown that the detrimental effects of UV-B radiation on microalgae are always mediated by free radicals which induce oxidative stress (Janknegt et al 2008;Wang et al 2007). Fortunately antioxidant systems in microalgae can scavenge reactive oxygen species (ROS), including the antioxidant molecules such as carotenoids, tocopherols, ascorbate, and reduced glutathione, and antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) (Janknegt et al 2008;Zhang et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Effect of UV-B radiation on the growth and antioxidant enzymes of Antarctic sea ice microalgae Chlamydomonas sp. ICE-L

et al. 2009

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The effect of ultraviolet-B (UV-B) radiation on Antarctic phytoplankton has become an attractive ecological issue as a result of annual springtime ozone depletion. The effects of UV-B radiation on the growth and antioxidant enzymes were investigated using Antarctic sea ice microalgae Chlamydomonas sp. ICE-L as the material in this study. The results demonstrated that UV-B radiation could notably inhibit the growth, especially at high UV-B radiation intensity (70 lW cm -2 ). Malondialdehyde and O 2 Á-content in ICE-L increased rapidly in early days (1-3 days) exposed to UV-B radiation enhancement, then decreased rapidly. In the stress of UV-B radiation enhancement, the superoxide dismutase, peroxidase and Catalase activities of 1-4 days in ICE-L were obviously higher than those in the control, and their activities became higher at high UV-B radiation intensity (70 lW cm -2 ). These enzymes activity of 7 days would kept stable at low UV-B radiation intensity (35 lW cm -2 ), but kept high level at high UV-B radiation intensity (70 lW cm -2 ). However, the ascorbate peroxidase activity in ICE-L kept stable under the stress of UV-B radiation enhancement. The above experimental results indicated that the antioxidant enzyme system played an important role in the adaptation of Antarctic ice microalgae under the UV-B radiation change of Antarctic ecosystems.

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“…For example, to tackle the detrimental effect of UVB radiation mediated via reactive oxygen species (ROS) Shi et al, 2005), cyanobacteria have developed efficient ROS scavenging systems, including small-molecule antioxidants and antioxidant enzymes (Malanga et al, 1999;Tyagi et al, 2003;Wang et al, 2007). We have previously shown that antioxidant molecules, such as ascorbate and reduced glutathione, as well as the activities of the antioxidant enzymes such as superoxide dismutase, catalase, and peroxidase, are significantly expressed in Nostoc sp.…”

Section: Introductionmentioning

confidence: 99%

Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp. in Response to UVB Radiation

et al. 2010

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Cyanobacteria are capable of tolerating environmental extremes. To survive in extreme environments, cyanobacteria have developed the capability to adapt to a variety of stresses. For example, cyanobacteria have adopted a number of strategies with which to survive UV stress, including expression of UV-screening pigments and antioxidant systems. We have previously shown that several antioxidants are significantly expressed in Nostoc sp. by UVB irradiation. We report here that the content of UV-responsive biomarkers such as b-carotene and scytonemin can be easily detected by Fourier transform Raman spectroscopy with use of a small sample size and that the content of b-carotene is dependant on the UVB intensity and exposure time. Our results indicate that Raman spectroscopy may be a helpful tool to analyze UV-protective molecules of cyanobacterium in astrobiological studies without access to large sample sizes and complicated extractions, which are needed by other analytical techniques such as high-performance liquid chromatography and mass spectrometry.

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The response of antioxidant systems in Nostoc sphaeroides against UV-B radiation and the protective effects of exogenous antioxidants

Cited by 36 publications

References 50 publications

The combined effects of UV-B radiation and herbicides on photosynthesis, antioxidant enzymes and DNA damage in two bloom-forming cyanobacteria

The combined effects of UV-B radiation and herbicides on photosynthesis, antioxidant enzymes and DNA damage in two bloom-forming cyanobacteria

Effect of UV-B radiation on the growth and antioxidant enzymes of Antarctic sea ice microalgae Chlamydomonas sp. ICE-L

Raman Spectroscopic Analysis of a Desert Cyanobacterium Nostoc sp. in Response to UVB Radiation

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