The Response of Breast Cancer Cells to Mesenchymal Stem Cells

Orciani, Monia; Bolletta, Elisa; Campanati, Anna; Benedetto, Giovanni Di; Primio, Roberto Di

doi:10.1097/prs.0000000000000665

Cited by 10 publications

(9 citation statements)

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“…29 During the acute phase, the strong production of IL-4 induces eosinophils to release IL-6 and TGF-b. 28 These molecules in turn support Th17 activation with high production of IL-17 members. The involvement of IL-6, TGF-b and predominantly IL-12 marks the passage to the chronic phase of AD, which is characterized mainly by a Th1/Th17 phenotype, and it has a crucial role in the process of differentiation of native T cells into Th1 cells.…”

Section: Discussionmentioning

confidence: 99%

“…The parameter threshold cycle (Ct) was defined as the cycle number at which the first detectable increase above the threshold in fluorescence was observed. Quantification of mRNA expression was calculated with the 2 ÀDDCt method, 28 where DCt = Ct (gene of interest) À Ct (control gene), and D(DCt) = DCt (C-MSCs) À DCt (AD-MSCs). The values of the relative expression of genes of interest are given as the mean AE SD over three independent experiments.…”

Section: Numbermentioning

confidence: 99%

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T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Section: Numbermentioning

confidence: 99%

T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem

et al. 2017

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“…Many studies have found that MSCs possess immunoregulatory capabilities . The immunosuppressive ability of MSCs has been studied both in vitro and in vivo over the last few years, but the detailed mechanisms of their immunosuppression remain controversial .…”

Section: Discussionmentioning

confidence: 99%

TNF‐α inhibitors reduce the pathological Th₁–Th₁₇/Th₂ imbalance in cutaneous mesenchymal stem cells of psoriasis patients

Campanati

Orciani

Lazzarini

et al. 2016

Experimental Dermatology

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Psoriasis is a disease characterized by an imbalance between Th and Th and Th inflammatory axes, in which cutaneous mesenchymal stem cells (MSCs) are early involved, as they show a greater relative expression of several genes encoding for Th and Th cytokines. Therapeutic implications of TNF-α inhibitors on differentiated skin cells have been largely described in psoriasis; however, their effects on MSCs derived from patients with psoriasis have been only partially described. The aim of this work was to evaluate the effect of TNF-α inhibitors on cytokine milieu expressed by MSCs isolated from the skin of patients with psoriasis. Resident MSCs from skin of patients with psoriasis and healthy subjects have been isolated, characterized and profiled by PCR and ELISA for the expression of 22 cytokines involved in Th , Th and Th pathways, both before and after 12 weeks therapy with TNF-α inhibitors. The administration of TNF-α inhibitors for 12-weeks acts on MSCs as follows: it reduces the expression of several Th -Th cytokines whose levels are elevated at baseline (IL-6, IL-8, IL-12, IL-23A, IFN-γ, TNF-α, CCL2, CCL20, CXCL2, CXCL5, IL-17A, IL-17C, IL-17F, IL-21, G-CSF). Similarly, it enhances the expression of several Th cytokines which are underexpressed at baseline (IL-2, IL-4, IL-5), reducing the expression of those overexpressed at baseline (TGF-β and IL-13). TNF-α inhibitors could contribute to reduce the pathological imbalance between the Th -Th vs Th axis in MSCs of patients with psoriasis.

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“…Data were acquired using CellQUEST software (Becton Dickinson). For the differentiation potential [14], cells were maintained in Stempro V R osteogenesis, chondrogenesis and adipogenesis differentiation kits (GIBCO, Grand Island, NY, USA). Cells in MSCGM were used as negative control.…”

Section: Isolation Culture and Characterization Of Mscsmentioning

confidence: 99%

“…The amount of mRNA detected in mock and cocultured PsO-MSCs was calculated as X-fold with respect to H-MSCs (expressed as 1) by the 2 2DDCt method [14], where DCt 5 Ct (gene of interest)-Ct (control gene) and D (DCt) 5 DCt (PsO-MSCs alone or co-cultured)-DCt (H-MSCs). X-fold was calculated for the selected genes in all 22 samples of mock MSCs (11 H-MScs and 11 PsO-MSCs) and in the 11 co-cultures (matched randomly).…”

Section: Rt-pcr and Enzyme-linked Immunosorbent Assay (Elisa) Arraymentioning

confidence: 99%

Indirect co-cultures of healthy mesenchymal stem cells restore the physiological phenotypical profile of psoriatic mesenchymal stem cells

Campanati

Orciani

Sorgentoni

et al. 2018

Clinical and Experimental Immunology

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Psoriasis microenvironment, characterized by an imbalance between T helper type 1 (Th1)/Th17 and Th2 cytokines and also influences the mesenchymal stem cells (MSCs) phenotypical profile. MSCs from healthy donors (H-MSCs) can exert a strong paracrine effect by secreting active soluble factors, able to modulate the inflammation in the microenvironment. To evaluate the influence of H-MSCs on MSCs from psoriatic patients (PsO-MSCs), H-MSCs and PsO-MSCs were isolated and characterized. Indirect co-culture of H-MSCs with PsO-MSCs was performed; effects on proliferation and expression of cytokines linked to Th1/Th17 and Th2 pathways were assayed before and after co-culture. The results show that before co-culture, proliferation of PsO-MSCs was significantly higher than H-MSCs (P < 0·05) and the levels of secreted cytokines confirmed the imbalance of Th1/Th17 versus the Th2 axis. After co-culture of H-MSCs with PsO-MSCs, healthy MSCs seem to exert a 'positive' influence on PsO-MSCs, driving the inflammatory phenotypical profile of PsO-MSCs towards a physiological pattern. The proliferation rate decreased towards values nearer to those observed in H-MSCs and the secretion of the cytokines that mostly identified the inflammatory microenvironment that characterized psoriasis, such as interleukin (IL)-6, IL-12, IL-13, IL-17A, tumour necrosis factor (TNF)-α and granulocyte-macrophage colony-stimulating factor (G-CSF), is significantly lower in co-cultured PsO-MSCs than in individually cultured PSO-MSCs (P at least < 0·05). In conclusion, our preliminary results seem to provide an intriguing molecular explanation for the ever-increasing evidence of therapeutic efficacy of allogeneic MSCs infusion in psoriatic patients.

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The Response of Breast Cancer Cells to Mesenchymal Stem Cells

Cited by 10 publications

References 4 publications

T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem

T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem

TNF‐α inhibitors reduce the pathological Th₁–Th₁₇/Th₂ imbalance in cutaneous mesenchymal stem cells of psoriasis patients

Indirect co-cultures of healthy mesenchymal stem cells restore the physiological phenotypical profile of psoriatic mesenchymal stem cells

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The Response of Breast Cancer Cells to Mesenchymal Stem Cells

Cited by 10 publications

References 4 publications

T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem

T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem

TNF‐α inhibitors reduce the pathological Th1–Th17/Th2 imbalance in cutaneous mesenchymal stem cells of psoriasis patients

Indirect co-cultures of healthy mesenchymal stem cells restore the physiological phenotypical profile of psoriatic mesenchymal stem cells

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TNF‐α inhibitors reduce the pathological Th₁–Th₁₇/Th₂ imbalance in cutaneous mesenchymal stem cells of psoriasis patients