The response of castrated male rats to the injection of testicular hormone

Korenchevsky, V.; Dennison, Marjorie; Schalit, Rosa

doi:10.1042/bj0261306

Cited by 34 publications

(20 citation statements)

References 2 publications

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“…In this connection, Korenchevsky, Dennison and Schalit [1932] state that ". ..when the testicular hormone is injected dissolved in oil uncertain amounts of unabsorbed oil were always found in the subcutaneous tissue at the places of the more recent injections.…”

Section: Recovery Of Cestrin From Unabsorbed Oilmentioning

confidence: 99%

Note on the subcutaneous absorption of oils by rats and mice, with special reference to the assay of œstrin

Deanesly¹,

Parkes²

1933

The Journal of Physiology

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OIL has often been used as a medium for the subcutaneous administration of substances insoluble in water, or most effective when absorbed slowly. Crude preparations of the gonad hormones are themselves oily, while purer or crystalline preparations are frequently dissolved in oil for injection. Olive, sesame and castor oils are most commonly used for this purpose. During some recent work on cestrin in castor oil and testis hormone in sesame oil it became apparent that absorption was very incomplete. The injected animals were therefore carefully skinned and the subcutaneous tissue examined. Pockets of oil were found to persist for many days (sesame oil) or even weeks (castor oil) after the injection of quite small amounts. The experiments described below were then carried out.METHODS. Varying amounts of olive, sesame and castor oils were injected into rats and mice. After the first few experiments, the oils were coloured with Scharlach R to assist detection at autopsy. The injections were made with a fine needle under the skin of the middle of the back. The animals were killed at intervals after injection, and the skin removed with the subcutaneous tissues attached, fixed and preserved in formalin. Careful dissection was required to avoid bursting the pocket of oil. The amount of oil remaining at autopsy was estimated approximately by comparison with known quantities injected into an animal which was killed immediately afterwards.The experimental results are shown in Table I. 1 Foulerton Student of the Royal Society.

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Section: Recovery Of Cestrin From Unabsorbed Oilmentioning

confidence: 99%

Note on the subcutaneous absorption of oils by rats and mice, with special reference to the assay of œstrin

Deanesly¹,

Parkes²

1933

The Journal of Physiology

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“…L'essai date des années 30 et il a été modifié dans les années 40 par l'inclusion de muscles de l'appareil reproducteur mâle qui répondent aux androgènes (2) (9)(10)(11)(12)(13)(14)(15). Au cours des années 60, plus de 700 androgènes putatifs ont été évalués à l'aide d'une version normalisée du mode opératoire (2) (14), et l'utilisation de l'essai a été considérée comme une méthode standard aussi bien pour les androgènes que pour les antiandrogènes pendant les années 60 (2) (15).…”

unclassified

“…De la même manière, des effets indésirables associés à des profils (anti)androgéniques peuvent être observés dans des essais de niveau 5, et nécessiter une évaluation visant à déterminer si une substance agit par un mode d'action endocrine. 10. Il est admis que tous les protocoles faisant appel à des animaux respectent les normes locales en matière de bien-être animal ; les descriptifs des soins et traitements figurant ci-après constituent donc des normes minimales auxquelles se substitueront les réglementations locales.…”

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Essai No. 441: Bio-essai de Hershberger sur le rat

2009

Lignes Directrices De l'OCDE Pour Les Essais De Produits Chimiques, Section 4

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“…Effect of corticosteroid and testosterone Marked atrophy of the thymus and the inhibition of weight-increase of the salivary glands were observed at the same time by the administration of some antithyroid drugs. Although this change in the thymus might be explained by hypofunction of the thyroid, there are differences of opinions regarding the relation between the thyroid and thymus (Carriere et al, 1938;Frenkel-Conrat et al, 1940;Reinhardt and Weinman, 1942;Gregoire, 1949;Boatman et al, 1951;Dougherty, 1952 (Ingle, 1940;Antopol, 1950;Ingle and Meeks, 1952) and sexhormones (Korenchevsky et al, 1932;Li and Evans, 1948;Money et al, 1952) Effect of extirpation and duct-ligation of the salivary glands In contrast with the above study,the weight of the thymus after a given period of salivary-ectomy or duct-ligation of the salivary glands was compared to solve the question as to whether the hypofunction of the salivary glands is related to the atrophy of the thymus.As shown in Table 3 …”

Section: Animalsmentioning

confidence: 99%