The response of muscle interstitial F2-isoprostane (8-ISO-PGF2α) during dynamic muscle contractions in humans

Karamouzis, Ioannis; Christoulas, Kosmas; Grekas, Dimitrios; Giannoulis, Kl; Vamvakoudis, Efstratios; Mandroukas, Konstantinos

doi:10.1016/j.plefa.2003.12.006

Cited by 20 publications

(29 citation statements)

References 20 publications

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“…69,70 In humans, cycling exercise at ∼85% VO 2max significantly increases skeletal muscle GSSG. 71 By using interstitial dialysis techniques, Karamouzis et al 72 have shown cycling at ∼70% VO 2max is sufficient to significantly increase skeletal muscle F2-isoprostane levels. Thus, it would appear that moderate-to-high intensity endurance exercise is sufficient to induce skeletal muscle oxidative stress.…”

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confidence: 99%

Skeletal muscle reactive oxygen species: A target of good cop/bad cop for exercise and disease

Mason

Wadley

2014

Redox Report

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Metabolic stresses associated with disease, ageing, and exercise increase the levels of reactive oxygen species (ROS) in skeletal muscle. These ROS have been linked mechanistically to adaptations in skeletal muscle that can be favourable (i.e. in response to exercise) or detrimental (i.e. in response to disease). The magnitude, duration (acute versus chronic), and cellular origin of the ROS are important underlying factors in determining the metabolic perturbations associated with the ROS produced in skeletal muscle. In particular, insulin resistance has been linked to excess ROS production in skeletal muscle mitochondria. A chronic excess of mitochondrial ROS can impair normal insulin signalling pathways and glucose disposal in skeletal muscle. In contrast, ROS produced in skeletal muscle in response to exercise has been linked to beneficial metabolic adaptations including mitochondrial biogenesis and muscle hypertrophy. Moreover, unlike insulin resistance, exercise-induced ROS appears to be primarily of nonmitochondrial origin. The present review summarizes the diverse ROS-targeted metabolic outcomes associated with insulin resistance versus exercise in skeletal muscle, thus, presenting two contrasting perspectives of pathologically harmful versus physiologically beneficial ROS. Here, we discuss the key sites of ROS production during exercise and the effect of ROS in skeletal muscle of people with type 2 diabetes.

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confidence: 99%

Skeletal muscle reactive oxygen species: A target of good cop/bad cop for exercise and disease

Mason

Wadley

2014

Redox Report

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“…The performance characteristics of this immunoassay method were described in our previous studies (12)(13).…”

Section: Methodsmentioning

confidence: 99%

“…a.). The biological fluid levels of the 15-F2t-Isoprostane (15-F 2t-IsoP) are regarded as being the most significant biochemical index of oxidative stress (12)(13)(14)(15). Some studies have measured 15-F 2t-IsoP in the plasma and urine of children with autism (1,5,7,11).…”

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confidence: 99%

Saliva Levels of 15-F_2t-Isoprostane as Biomarker of Lipid Peroxidation in Autistic Children

Karamouzi¹,

Kovachev

Karamouzis

et al. 2007

Eur J Inflamm

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Autism is a complex neurodevelopmental disorder that usually presents itself in early childhood and is thought to be influenced by genetic and environmental factors. Oxidative stress has been implicated in the etiology of neurological, neurodevelopment and neuropsychiatric disorders including Parkinson's and Alzheimer disease, Down's syndrome and autism. The biological fluid levels of the 15-F2t-Isoprostane (15-F2t-IsoP) are regarded as being the most significant biochemical index of oxidative stress. There are some studies that measured 15-F2t-IsoP in the plasma and urine of children with autism. However 15-F2t-IsoP has not been measured in the saliva of children with autism. This procedure is less stressful for the children with autism. The purpose of this study is to evaluate saliva levels of 15-F2t-IsoP in children diagnosed with autism. Saliva levels of 15-F2t-IsoP were determined in 18 children with autism age: 6.6±1.6 years and 21 healthy controls age: 7.5±1.4 years. Compared to the control group, the children with autism had significantly higher saliva levels of 15-F2t-IsoP. From the above-mentioned it is concluded that a) lipid peroxidative biomarker 15-F2t-IsoP is increased in autistic children, and b) saliva is the proper (optimal) biological fluid for the evaluation the lipid peroxidation in autistic children.

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“…The dialysate fluid contains no enzymes or proteins, hence this technique allows the isolation, purification and quantitative measurements of these compounds in the interstitial space of various tissues. 17,18 The eicosanoid hormones (derivatives of arachidonic acid, prostaglandins, prostacyclins, thromboxanes and F2-isoprostanes) have low molecular weight (<5.000 Da) and can thus be isolated from the IF of the muscle with the use of this microdialysis technique. After the isolation of these locally produced hormones, it is easy to measure their concentration in the IF via a sensitive immunoassay method such as Radioimmunoassay (RIA) or Enzyme Immunoassay (EIA).…”

mentioning

confidence: 99%

“…[17][18][19] In a previous study using the microdialysis technique, we demonstrated the existence of 8-iso-PGF2α in the IF of the skeletal muscles of healthy individuals. 18 In the present study, we determined the values of 8-iso-PGF2α as an index of lipid peroxidation in plasma and IF thoughout hemodialysis, also looking for a possible gradient between plasma and IF in patients with end stage renal failure.…”

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confidence: 99%

Enhanced oxidative stress with a gradient between plasma and muscle interstitial fluid in patients with end stage renal failure on hemodialysis

Karamouzis¹,

Grekas²,

Karamouzis³

et al. 2008

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The response of muscle interstitial F2-isoprostane (8-ISO-PGF2α) during dynamic muscle contractions in humans

Cited by 20 publications

References 20 publications

Skeletal muscle reactive oxygen species: A target of good cop/bad cop for exercise and disease

Skeletal muscle reactive oxygen species: A target of good cop/bad cop for exercise and disease

Saliva Levels of 15-F_2t-Isoprostane as Biomarker of Lipid Peroxidation in Autistic Children

Enhanced oxidative stress with a gradient between plasma and muscle interstitial fluid in patients with end stage renal failure on hemodialysis

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The response of muscle interstitial F2-isoprostane (8-ISO-PGF2α) during dynamic muscle contractions in humans

Cited by 20 publications

References 20 publications

Skeletal muscle reactive oxygen species: A target of good cop/bad cop for exercise and disease

Skeletal muscle reactive oxygen species: A target of good cop/bad cop for exercise and disease

Saliva Levels of 15-F2t-Isoprostane as Biomarker of Lipid Peroxidation in Autistic Children

Enhanced oxidative stress with a gradient between plasma and muscle interstitial fluid in patients with end stage renal failure on hemodialysis

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Saliva Levels of 15-F_2t-Isoprostane as Biomarker of Lipid Peroxidation in Autistic Children