The response of the poplar transcriptome to wounding and subsequent infection by a viral pathogen

Smith, Caroline M.; Rodriguez‐Buey, Marisa; Karlsson, Jan; Campbell, Malcolm M.

doi:10.1111/j.1469-8137.2004.01151.x

Cited by 60 publications

(48 citation statements)

References 80 publications

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“…The RNA samples from different leaves were pooled for Yt 1 , Yt 2 and Mt 1 . The cDNA synthesis, probe preparation, hybridisation conditions, data collection and data analysis were done according to Smith et al (2004). The cDNA was prepared from 30 lg of total RNA.…”

Section: Microarray Analysismentioning

confidence: 99%

Nocturnal changes in leaf growth of Populus deltoides are controlled by cytoplasmic growth

Matsubara

Hurry

Druart

et al. 2005

Planta

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Growing leaves do not expand at a constant rate but exhibit pronounced diel growth rhythms. However, the mechanisms giving rise to distinct diel growth dynamics in different species are still largely unknown. As a first step towards identifying genes controlling rate and timing of leaf growth, we analysed the transcriptomes of rapidly expanding and fully expanded leaves of Populus deltoides Bartr. ex. Marsh at points of high and low expansion at night. Tissues with well defined temporal growth rates were harvested using an online growth-monitoring system based on a digital image sequence processing method developed for quantitative mapping of dicot leaf growth. Unlike plants studied previously, leaf growth in P. deltoides was characterised by lack of a base-tip gradient across the lamina, and by maximal and minimal growth at dusk and dawn, respectively. Microarray analysis revealed that the nocturnal decline in growth coincided with a concerted down-regulation of ribosomal protein genes, indicating deceleration of cytoplasmic growth. In a subsequent time-course experiment, Northern blotting and real-time RT-PCR confirmed that the ribosomal protein gene RPL12 and a cell-cycle gene H2B were down-regulated after midnight following a decrease in cellular carbohydrate concentrations. Thus, we propose that the spatio-temporal growth pattern in leaves of P. deltoides primarily arises from cytoplasmic growth whose activity increases from afternoon to midnight and thereafter decreases in this species.

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Section: Microarray Analysismentioning

confidence: 99%

Nocturnal changes in leaf growth of Populus deltoides are controlled by cytoplasmic growth

Matsubara

Hurry

Druart

et al. 2005

Planta

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“…These fungi show a high level of compatibility with their host, but the reasons for the obligate biotroph status remains unknown (Mendgen and Hahn, 2002). To date, only sparse molecular data were obtained on defense mechanisms in poplar, and published data concern interactions with herbivores or viruses (Christopher et al, 2004;Smith et al, 2004;Major and Constabel, 2006;Ralph et al, 2006a). Molecular responses to Melampsora spp.…”

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confidence: 99%

Transcript Profiling of Poplar Leaves upon Infection with Compatible and Incompatible Strains of the Foliar RustMelampsora larici-populina

et al. 2007

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To understand key processes governing defense mechanisms in poplar (Populus spp.) upon infection with the rust fungus Melampsora larici-populina, we used combined histological and molecular techniques to describe the infection of Populus trichocarpa 3 Populus deltoides 'Beaupré' leaves by compatible and incompatible fungal strains. Striking differences in hosttissue infection were observed after 48-h postinoculation (hpi) between compatible and incompatible interactions. No reactive oxygen species production could be detected at infection sites, while a strong accumulation of monolignols occurred in the incompatible interaction after 48 hpi, indicating a late plant response once the fungus already penetrated host cells to form haustorial infection structures. P. trichocarpa whole-genome expression oligoarrays and sequencing of cDNAs were used to determine changes in gene expression in both interactions at 48 hpi. Temporal expression profiling of infection-regulated transcripts was further compared by cDNA arrays and reverse transcription-quantitative polymerase chain reaction. Among 1,730 significantly differentially expressed transcripts in the incompatible interaction, 150 showed an increase in concentration $3-fold, whereas 62 were decreased by $3-fold. Regulated transcripts corresponded to known genes targeted by R genes in plant pathosystems, such as inositol-3-P synthase, glutathione S-transferases, and pathogenesis-related proteins. However, the transcript showing the highest rust-induced up-regulation encodes a putative secreted protein with no known function. In contrast, only a few transcripts showed an altered expression in the compatible interaction, suggesting a delay in defense response between incompatible and compatible interactions in poplar. This comprehensive analysis of early molecular responses of poplar to M. larici-populina infection identified key genes that likely contain the fungus proliferation in planta.

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“…However the role of MTs in this process has not been conclusively shown in plants; under Cu-stress condition, for example, MTs were highly downregulated. This downregulation has been observed previously in poplar under pathogens infection (Smith et al 2004) and has been interpreted as a mechanism to inducing a transient reactive oxygen species (ROS) accumulation necessary to trigger a general resistance response in plants.…”

Section: Transcriptomic and Proteomic Analysismentioning

confidence: 81%

Heavy Metals Stress on Poplar: Molecular and Anatomical Modifications

Sebastiani

Francini

Romeo

et al. 2013

Approaches to Plant Stress and Their Management

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Heavy metal stress responses vary from plant to plant depending on the type of heavy metals and require a coordinated interplay of complex physiological and biochemical processes, gene expression, protein modification and changes in metabolites compositions leading to proper stress signal and tolerance.Fast-growing tree species, such as poplar, have been studied as possible candidate in phytoremediation approaches to clean up soil or water polluted by organic and inorganic compounds. In particular poplar is known both for the ability to uptake (i.e. phytoextraction) and to stabilise heavy metals (i.e. phytostabilisation) into their tissues, thus reducing the mobility of these contaminants in the soil profile. Compared to other plant species, poplar trees have several advantageous characteristics, such as deeper root system, higher transpiration activity, and productivity. Moreover, they produce economically valuable nonfood biomass exploitable both for wood and bioenergy production. Since the availability of the genome sequence of Populus trichocarpa and the development of highthroughput technologies, poplar has also emerged as the model system for tree biology studies.In this chapter, we examine the effects of heavy metals on anatomical traits and molecular machinery that are responsible for their accumulation and tolerance in poplar. Starting with this deeper molecular information, this chapter could provide new ideas for improving poplar trees with traits conferring heavy metals tolerance.

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The response of the poplar transcriptome to wounding and subsequent infection by a viral pathogen

Cited by 60 publications

References 80 publications

Nocturnal changes in leaf growth of Populus deltoides are controlled by cytoplasmic growth

Nocturnal changes in leaf growth of Populus deltoides are controlled by cytoplasmic growth

Transcript Profiling of Poplar Leaves upon Infection with Compatible and Incompatible Strains of the Foliar RustMelampsora larici-populina

Heavy Metals Stress on Poplar: Molecular and Anatomical Modifications

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