The Response of Vibrio- and Rhodobacter-Related Populations of the NW Mediterranean Sea to Additions of Dissolved Organic Matter, Phages, or Dilution

Weinbauer, Markus G.; Christen, Richard; Höfle, Manfred G.

doi:10.1007/s00248-006-9028-x

Cited by 24 publications

(22 citation statements)

References 48 publications

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“…Microcosm studies have shown that Vibrio spp. can grow faster than other prokaryotes due to their more efficient utilization of DOM, and thus may aid in their persistence in particle-free seawater (Mouriño-Pérez et al, 2003;Weinbauer et al, 2006). Our study has also revealed that the oceanic Vibrio populations correlated more strongly with DOC and DON than total prokaryotes represented by DAPI or CBC.…”

Section: Dom As An Important Regulator Of Vibrio and Other Bacterial supporting

confidence: 56%

“…These types of molecular analysis have revealed the frequent occurrence of Vibrio spp. in oceanic water, although other subgroups of bacteria, e.g., SAR11, Roseobacter, Alteromonas, and CytophagaFlavobacterium-Bacteroides can also be dominant (Eilers et al, 2000;Malmstrom et al, 2005;Weinbauer et al, 2006;Taniguchi and Hamasaki, 2008;Schattenhofer et al, 2009;Wietz et al, 2010). However, culture independent techniques cannot provide information on the active, cultivable portion of a species.…”

Section: Introductionmentioning

confidence: 99%

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Biogeochemical controls on the bacterial populations in the eastern Atlantic Ocean

et al. 2011

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Abstract.Little is known about bacterial dynamics in the oligotrophic ocean, particularly about cultivable bacteria. We examined the abundance of total and cultivable bacteria in relation to changes in biogeochemical conditions in the eastern Atlantic Ocean with special regard to Vibrio spp., a group of bacteria that can cause diseases in human and aquatic organisms. Surface, deep water and plankton (<20 µm, 20-55 µm and >55 µm) samples were collected between 50 • N and 24 • S. Chlorophyll-a was very low (<0.3 µg l −1 ) in most areas of the nutrient-poor Atlantic, except at a few locations near upwelling regions. In surface water, dissolved organic carbon (DOC) and nitrogen (DON) concentrations were 64-95 µM C and 2-10 µM N accounting for ≥90 % and ≥76 % of total organic C and N, respectively. DOC and DON gradually decreased to ∼45 µM C and <5 µM N in the bottom water. In the surface layer, culture independent total bacteria and other prokaryotes represented by 4 -6-diamidino-2-phenylindole (DAPI) counts, ranged mostly between 10 7 and 10 8 cells l −1 , while cultivable bacterial counts (CBC) and Vibrio spp. were found at concentrations of 10 4 -10 7 and 10 2 -10 5 colony forming units (CFU) l −1 , respectively. Most bacteria (>99 %) were found in the nanoplankton fraction (<20 µm), however, bacterial abundance did not correlate with suspended particulates (chlorophyll-a, particulateCorrespondence to: R. J. Lara (ruben.lara@zmt-bremen.de) organic C [POC] and N [PON]). Instead, we found a highly significant correlation between bacterial abundance and temperature (p < 0.001) and a significant correlation with DOC and DON (p < 0.005 and < 0.01, respectively). In comparison to CBC and DAPI-stained prokaryotes, cultivable Vibrio showed a stronger and highly significant correlation with DOC and DON (p < 0.0005 and p < 0.005, respectively). In cold waters of the mesopelagic and abyssal zones, CBC was 50 to 100-times lower than in the surface layer; however, cultivable Vibrio spp. could be isolated from the bathypelagic zone and even near the seafloor (average ∼10 CFU l −1 ). The depth-wise decrease in CBC and Vibrio coincided with the decrease in both DOC and POC. Our study indicates that Vibrio and other bacteria may largely depend on dissolved organic matter to survive in nutrient-poor oceanic habitats.

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Section: Dom As An Important Regulator Of Vibrio and Other Bacterial supporting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Biogeochemical controls on the bacterial populations in the eastern Atlantic Ocean

et al. 2011

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“…For example, lysis of bacterial cells provides dissolved organic matter (substrate) which can enhance the growth of other bacterial groups (Weinbauer & Rassoulzadegan 2004, Middelboe & Jørgensen 2006. However, decreases in bacterial growth due to viral lysis have been found to vary among different phylogenetic groups (Weinbauer et al 2006). In coastal Oregon, Bouvier & del Giorgio (2007) demonstrated that Betaproteobacteria dominated the shelf region in virus-depleted treatments while Gammaproteobacteria comprised the largest proportion of the bacterial community at the oligotrophic station, whether or not viruses were present.…”

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confidence: 99%

Effect of top-down control on cell-specific activity and diversity of active marine bacterioplankton

Longnecker

Wilson²,

Sherr³

et al. 2010

Aquat. Microb. Ecol.

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Mortality processes such as grazing and viral lysis can alter both phylogenetic diversity and cell-specific activity of bacterioplankton. We conducted experiments to examine the effect of reduction in grazer or viral abundance on metabolically active bacterioplankton at a eutrophic shelf station and an oligotrophic basin station. Leucine assimilation was used as a proxy to characterize metabolically active bacterial cells. The phylogenetic affiliation of marine bacterioplankton assimilating leucine was identified with fluorescence in situ hybridization and microautoradiography. Flow cytometric sorting of leucine-labeled cells quantified cell-specific activity of high nucleic acid (HNA) and low nucleic acid cells. The activity and diversity of the bacterial community at the oligotrophic station was more responsive to a reduction in mortality compared to the community at the eutrophic station. HNA cells at the oligotrophic station showed a 4-fold increase in cell-specific leucine incorporation when the abundance of flagellates was reduced and an 8-fold increase when the abundance of flagellates, viruses, and bacterial cells was reduced. The abundance of active Alphaproteobacteria increased at the oligotrophic station when either grazer or viral abundance was reduced. Activity responses were less striking at the eutrophic station, and the abundance of active Gammaproteobacteria showed a greater increase with reduced flagellates compared to the treatment with reduced flagellates, viruses, and bacterioplankton. Our results indicate both the presence and type of mortality process play a key role in structuring a bacterial community, and this effect varies in ecosystems of differing trophic state.KEY WORDS: Marine bacterioplankton · Top-down control · Leucine incorporation · Flow cytometry · Microautoradiography · Fluorescence in situ hybridization · Grazing · Viral lysis Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 58: [153][154][155][156][157][158][159][160][161][162][163][164][165] 2010 Protocols involving a combination of fluorescence in situ hybridization (FISH) with microautoradiography allow identification of metabolically active phylogenetic groups within marine bacterioplankton (e.g. Ouverney & Fuhrman 1999, Cottrell & Kirchman 2000. This approach has provided important insights into both spatial (Elifantz et al. 2007, Varela et al. 2008) and temporal (Alonso-Sáez & Gasol 2007) variability in the diversity of active bacterial cells. In previous work in the Oregon upwelling system, we found little spatial variability in the diversity of active cells (Longnecker et al. 2005), although the abundance of active cells increased with ecosystem trophic state (Longnecker et al. 2006a). These findings raised the question of the relative importance of top-down (mortality) versus bottom-up (substrate availability) processes in eutrophic compared to oligotrophic marine ecosystems.In addition to the qualitative information derived from FISH analysis, flow cyto...

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“…2000; Ghiglione et al. 2005) and bacteria present in those communities may exhibit different life strategies (Weinbauer et al. 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of a transient perturbation on marine bacterial communities with contrasting history

Zemb¹,

West²,

Bourrain³

et al. 2010

Journal of Applied Microbiology

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Aims: To evaluate the importance of the bacterial composition on the resilience of the organic matter assimilation in the sea. Methods and Results: Chemostats were inoculated with coastal and offshore bacterial communities. Bacterial density and protein synthesis increased before stabilizing, and this response to confinement was more marked in the offshore chemostats. Before the toluene perturbation the community structure in the coastal chemostats remained complex whereas the offshore chemostats became dominated by Alteromonas sp. After the perturbation, bacterial protein synthesis was inhibited before peaking briefly at a level fivefold to that observed before the perturbation and then stabilizing at a level comparable to that before the perturbation. Alteromonas dominated both the coastal and the offshore communities immediately after the perturbation and the coastal communities did not recover their initial complexity. Conclusions: Cell lysis induced by the toluene perturbation favoured the growth of Alteromonas which could initiate growth rapidly in response to the nutrient pulse. Despite their different community structure in situ, the resilience of protein synthesis of coastal and offshore bacterial communities was dependent on Alteromonas, which dominated in the chemostats. Significance and Impact of the Study: Here we show that although Alteromonas sp. dominated in artificial offshore and coastal communities in chemostats, their response time to the shock was different. This suggests that future perturbation studies on resilience in the marine environment should take account of ecosystem history.

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The Response of Vibrio- and Rhodobacter-Related Populations of the NW Mediterranean Sea to Additions of Dissolved Organic Matter, Phages, or Dilution

Cited by 24 publications

References 48 publications

Biogeochemical controls on the bacterial populations in the eastern Atlantic Ocean

Biogeochemical controls on the bacterial populations in the eastern Atlantic Ocean

Effect of top-down control on cell-specific activity and diversity of active marine bacterioplankton

Effect of a transient perturbation on marine bacterial communities with contrasting history

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