2020
DOI: 10.1016/j.micpath.2020.104197
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The response regulator ArcA enhances biofilm formation in the vpsT manner under the anaerobic condition in Vibrio cholerae

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Cited by 20 publications
(12 citation statements)
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“…ArcA is a global transcription regulator in the Enterobacteriaceae , which controls the expression of genes involved in several different pathways 39 , 40 . For instance, ArcA directly regulates the expression of vpsT involved in biofilm formation of V. cholerae 41 , which contributes to bacterial intestinal colonization 42 , 43 . It is highly likely that the influence of ArcA on the virulence of V. cholerae might only be partially dependent of its regulation on fruI and fruT expression.…”
Section: Resultsmentioning
confidence: 99%
“…ArcA is a global transcription regulator in the Enterobacteriaceae , which controls the expression of genes involved in several different pathways 39 , 40 . For instance, ArcA directly regulates the expression of vpsT involved in biofilm formation of V. cholerae 41 , which contributes to bacterial intestinal colonization 42 , 43 . It is highly likely that the influence of ArcA on the virulence of V. cholerae might only be partially dependent of its regulation on fruI and fruT expression.…”
Section: Resultsmentioning
confidence: 99%
“…This suggests that inactivation of arcA impacts other adhesins important for biofilm formation, for example, the formation of fimbriae. ArcA has also been shown to regulate biofilm formation in several other species of bacteria (Sun et al., 2020; Xi et al., 2020; Yan et al., 2021). The data suggest that ArcA acts primarily as a positive regulator for emaA transcription either binding directly to the DNA or indirectly by competing with a negative regulator in response to changes in the environment.…”
Section: Discussionmentioning
confidence: 99%
“…A sequence encoding a ArcA/CytR/CRP-His 6 fusion protein was cloned into vector pET-28a, expressed in E. coli BL21 (DE3), and purified using an Ni–NTA-Sefinose Column in accordance with the protocol provided by the manufacturer [ 37 , 39 ]. EMSA was performed by adding increasing amounts of purified phosphorylated ArcA protein (0, 1.5, 3.0, 4.5, and 6.0 µM or 0, 0.6, 1.2, 1.8 and 2.4 µM) to cytR or flrA DNA fragments (50 ng) in a binding buffer [10 mM Tris–HCl (pH 8.0), 1 mM EDTA, 1 mM DTT, 50 mM KCl, 50 μg/mL BSA, 10% glycerol] supplemented with 20 nM acetyl phosphate [ 39 ], followed by incubation for 40 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%
“…A sequence encoding a ArcA/CytR/CRP-His 6 fusion protein was cloned into vector pET-28a, expressed in E. coli BL21 (DE3), and purified using an Ni–NTA-Sefinose Column in accordance with the protocol provided by the manufacturer [ 37 , 39 ]. EMSA was performed by adding increasing amounts of purified phosphorylated ArcA protein (0, 1.5, 3.0, 4.5, and 6.0 µM or 0, 0.6, 1.2, 1.8 and 2.4 µM) to cytR or flrA DNA fragments (50 ng) in a binding buffer [10 mM Tris–HCl (pH 8.0), 1 mM EDTA, 1 mM DTT, 50 mM KCl, 50 μg/mL BSA, 10% glycerol] supplemented with 20 nM acetyl phosphate [ 39 ], followed by incubation for 40 min at room temperature. Similarly, fliK DNA fragments (50 ng) were incubated with increasing amounts of 6 × His-tagged CytR or CRP protein (0, 1.2, 2.4, and 3.6 µM or 0, 2, 3, and 4 µM) in a binding buffer [20 mM Tris–HCl (pH 7.5), 50 mM KCl, 1 mM dithiothreitol, 200 µM cAMP, and 10% glycerol] [ 20 ], followed by incubation for 40 min at room temperature.…”
Section: Methodsmentioning
confidence: 99%