2006
DOI: 10.1128/jb.188.7.2636-2645.2006
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The Response Regulator CroR Modulates Expression of the Secreted Stress-Induced SalB Protein in Enterococcus faecalis

Abstract: . In this report, we provide genetic and biochemical evidence that salB encodes a secreted protein that is expressed from a monocistronic stress-inducible operon. Consistent with CroR being a direct transcriptional activator of the salB expression, CroR was found to bind to the salB promoter region in electrophoretic mobility shift assays. Interestingly, we provide evidence that SalB does not play a role in the intrinsic ␤-lactam resistance associated with CroRS. We also show that the CroRS system is able to r… Show more

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Cited by 45 publications
(58 citation statements)
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“…The protein complement supports known phenotypes of the salB mutant strain; moreover, it potentially defines roles for SalB. Previous studies determined that a salB mutant has reduced sensitivity to environmental stress and salB transcription is directly regulated by the two-component signal transduction system CroRS (14,18). The stimulus activating CroRS is unknown.…”
Section: Figmentioning
confidence: 67%
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“…The protein complement supports known phenotypes of the salB mutant strain; moreover, it potentially defines roles for SalB. Previous studies determined that a salB mutant has reduced sensitivity to environmental stress and salB transcription is directly regulated by the two-component signal transduction system CroRS (14,18). The stimulus activating CroRS is unknown.…”
Section: Figmentioning
confidence: 67%
“…Certainly, the morphology of a salB mutant and that of a divIVA (trans complementation) mutant of E. faecalis are strikingly similar (22). The modulation of SalB expression by the CroRS two-component signal transduction regulator links cell morphology with intrinsic ␤-lactam resistance (7,18). Recent analysis of PcsB in S. pneumoniae has provided the first evidence supporting a role for PcsB/SalB/SagA family proteins in the cell division complex via its interaction with FtsX (28).…”
Section: Figmentioning
confidence: 99%
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“…The resulting construction was electroporated into the E. coli M15pRep4 strain, creating the M15/pQE 30 cspR strain (see Table S1 in the supplemental material). Recombinant CspR protein was overproduced and purified as described by Muller et al (35).…”
Section: Methodsmentioning
confidence: 99%
“…CroR is capable of gene regulation, but thus far only three CroR-dependent genes have been described. These include croR itself, a gene encoding a secreted protein (salB), and the first gene of a predicted glutamine transport system (glnQ), although only croR plays a role in cephalosporin resistance (3,15,16). Therefore, although CroS and CroR seem to comprise a prototypical TCS involved in the response to cell wall stress, little is known about the CroRS-dependent molecular events that promote resistance to cell wall-targeting agents in E. faecalis.…”
mentioning
confidence: 99%