The Response Regulator PhoP Is Important for Survival under Conditions of Macrophage-Induced Stress and Virulence in
            <i>Yersinia pestis</i>

Oyston, Petra C. F.; Dorrell, Nick; Williams, Katrina; Li, Shu-Rui; Green, Michael; Titball, Richard W.; Wren, Brendan W.

doi:10.1128/iai.68.6.3419-3425.2000

Cited by 205 publications

(212 citation statements)

References 41 publications

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“…The latter regulates invasion genes on the pathogenicity island I, SPI-1 [29]. This regulatory system has also been identified in Yersinia pestis and was shown to be important for survival under conditions of macrophage-induced stress [51].…”

Section: Virulence Plasmidmentioning

confidence: 96%

Rhodococcus equi

2004

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-Rhodococcus equi is an important cause of subacute or chronic abscessating bronchopneumonia of foals up to 3-5 months of age. It shares the lipid-rich cell wall envelope characteristic of the mycolata, including Mycobacterium tuberculosis, as well as the ability of pathogenic members of this group to survive within macrophages. The possession of a large virulence plasmid in isolates recovered from pneumonic foals is crucial for virulence. The plasmid contains an 27 kb pathogenicity island (PI) that encodes seven related virulence-associated proteins (Vaps), including the immunodominant surface-expressed protein, VapA. Only PI genes are differentially expressed when the organism is grown in macrophages in vitro. Ten of the PI genes, including six Vap genes, have signal sequences, suggesting that they are exported from the cell to interact with the macrophage. Different PI genes are regulated by temperature, pH, iron, oxidative stress and probably also by magnesium, all environmental changes encountered after environmental R. equi are inhaled in dust and are ingested into macrophages in the lung. The basis of pathogenicity of R. equi is its ability to multiply in and eventually to destroy alveolar macrophages. Infectivity is largely or exclusively limited to cells of the monocyte-macrophage lineage. Current evidence suggests that infection of foals with virulent R. equi results in some foals in subversion of cellmediated immunity and development of an ineffective and sometimes lethal Th2-based immune response. Significant progress has been made recently in the development of R. equi-E. coli shuttle vectors, transformation and random and site specific mutagenesis procedures, all of which will be important in molecular dissection of the mechanisms by which R. equi subverts normal macrophage killing mechanisms and cell-mediated immunity.

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Section: Virulence Plasmidmentioning

confidence: 96%

Rhodococcus equi

2004

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“…TCS PhoP-PhoQ system of Salmonella and Yersinia pestis is responsible for the virulence within animal host cells [13][14][15]. PhoPPhoQ of X. oryzae pv.…”

Section: Discussionmentioning

confidence: 99%

“…Salmonella species are facultative intracellular pathogens that can infect a wide variety of animals causing different diseases [12]. A phoP mutant of the etiologic agent of bubonic plagues Yersinia pestis [13,14], reduced the abilities to survival within macrophages, and increased sensitivity to low pH, oxidative stress, and high osmolarity [15]. A phoP deficient of Shigella is hypersensitive to killing by neutrophils [16].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

Lin¹

2015

J Plant Pathol Microbiol

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Xylella fastidiosa (Xf) possess a two component regulatory system (TCS) PopP-PopQ which differentially regulates genes in response to environmental stimuli. To elucidate the role of popP in the pathogenicity of X. fastidiosa causing Pierce's disease (PD) of grapevine, a site-directed deletion method and chromosome-based genetic complementation strategy were employed to create popP deletion mutant XfΔpopP and its complementary strain XfΔpopP-C. In vitro studies showed that while all strains had similar growth curves, XfΔpopP showed significant reduction in cell-cell aggregation and cell-matrix adherence. Biofilm production of XfΔpopP was about 42% less than that of wild type X. fastidiosa and XfΔpopP-C. No symptoms were observed in grapevines inoculated with XfΔpopP, whereas grapevines inoculated with wild type X. fastidiosa and XfΔpopP-C showed typical PD symptoms. Several biofilm-related genes and genes involving protein secretary systems were down regulated in XfΔpopP in compared with wild type X. fastidiosa and XfΔpopP-C. These in vitro and in planta assay results provide strong evidence that the role of PopP is required for pathogenicity of X. fastidiosa on grapevine.

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“…Macrophage infection assays were performed using the J774A.1 mouse macrophage cell line using published methods (Oyston et al, 2000;Papp-Wallace & Maguire, 2008). For Y. pseudotuberculosis infections an m.o.i.…”

Section: Methodsmentioning

confidence: 99%

The importance of the magnesium transporter MgtB for virulence of Yersinia pseudotuberculosis and Yersinia pestis

et al. 2014

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Mg 2+ has been shown to be an important signal controlling gene regulation via the PhoPQ twocomponent regulatory system for a range of Gram-negative bacteria, including Yersinia pestis and Yersinia pseudotuberculosis. The magnesium ion transporter MgtB is part of the complex PhoPQ regulon, being upregulated in response to low Mg 2+ . Despite the presence of other Mg 2+ transport systems in Yersinia, inactivation of mgtB had a significant effect on the ability of the bacteria to scavenge this crucial ion. Whereas inactivation of PhoPQ is reported to adversely affect intracellular survival, we show that Y. pestis and Y. pseudotuberculosis DmgtB mutants survived equally as well as the respective parent strain within macrophages, although they were more sensitive to killing in the Galleria model of infection. Surprisingly, despite MgtB being only one member of the Mg 2+ stimulon and PhoPQ controlling the expression levels of a range of genes including mgtB, the Yersinia DmgtB mutants were more highly attenuated than the equivalent Yersinia DphoP mutants in mouse models of infection. MgtB may be a suitable target for development of novel antimicrobials, and investigation of its role may help elucidate the contribution of this component of the PhoPQ regulon to pathogenesis.

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The Response Regulator PhoP Is Important for Survival under Conditions of Macrophage-Induced Stress and Virulence in Yersinia pestis

Cited by 205 publications

References 41 publications

Rhodococcus equi

Rhodococcus equi

Characterization of Xylella fastidiosa popP Gene Required for Pathogenicitya

The importance of the magnesium transporter MgtB for virulence of Yersinia pseudotuberculosis and Yersinia pestis

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