The responses of Scrippsiella acuminata to the stresses of darkness: antioxidant activities and formation of pellicle cysts

Wang, Zhaohui; Xie, Changliang; Zhang, Jianneng; Ji, Shuanghui; Zhao, Jun; Nie, Xiaoqin

doi:10.1016/j.hal.2022.102239

Cited by 9 publications

(2 citation statements)

References 48 publications

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“…We measured the densities of G. smaydae and Heterocapsa rotundata as prey in a 100-L semi-continuous cultivation system under no aeration and continuous aeration conditions daily for 9 days. Furthermore, to determine optimal harvest conditions through centrifugation, we examined the harvesting efficiency and dry weights of fatty acids in G. smaydae + H. rotundata cells at different rotational speeds and at different flow rates of the pump frequently predominant in plankton assemblages (Jeong et al 2021, Kang et al 2023, and they produce useful biological materials including fatty acids, amino acids, functional pigments, antioxidants, and toxins (Hwang and Lu 2000, Jang et al 2017, Lim et al 2018, Wang et al 2022, Buchheim et al 2023. Therefore, marine microalgae have potential applications with regard to bioenergy resources, animal feed, human foods, health supplements, and pharmaceuticals (Atalah et al 2007, Fuentes-Grünewald et al 2009, Manirafasha et al 2016, Lim et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Effects of aeration and centrifugation conditions on omega-3 fatty acid production by the mixotrophic dinoflagellate Gymnodinium smaydae in a semi-continuous cultivation system on a pilot scale

You,

Jeong,

Park

et al. 2024

Algae

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High production and efficient harvesting of microalgae containing high omega-3 levels are critical concerns for industrial use. Aeration can elevate production of some microalgae by providing CO2 and O2. However, it may lower the production of others by generating shear stress, causing severe cell damage. The mixotrophic dinoflagellate Gymnodinium smaydae is a new, promising microalga for omega-3 fatty acid production owing to its high docosahexaenoic acid content, and determining optimal conditions and methods for high omega-3 fatty acid production and efficient harvest using G. smaydae is crucial for its commercial utilization. Therefore, to determine whether continuous aeration is required, we measured densities of G. smaydae and the dinoflagellate prey Heterocapsa rotundata in a 100-L semi-continuous cultivation system under no aeration and continuous aeration conditions daily for 9 days. Furthermore, to determine the optimal conditions for harvesting through centrifugation, different rotational speeds of the continuous centrifuge and different flow rates of the pump injecting G. smaydae + H. rotundata cells into the centrifuge were tested. Under continuous aeration, G. smaydaeproduction gradually decreased; however, without aeration, the production remained stable. Harvesting efficiency and the dry weights of omega-3 fatty acids of G. smaydae + H. rotundata cells at a rotational speed of 16,000 rpm were significantly higher than those at 2,000–8,000 rpm. However, these parameters did not significantly differ at injection pump flow rates of 1.0–4.0 L min-1. The results of the present study provide a basis for optimized production and harvest conditions for G. smaydae and other microalgae.

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Section: Introductionmentioning

confidence: 99%

Effects of aeration and centrifugation conditions on omega-3 fatty acid production by the mixotrophic dinoflagellate Gymnodinium smaydae in a semi-continuous cultivation system on a pilot scale

You,

Jeong,

Park

et al. 2024

Algae

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“…The armored dinoflagellate S. acuminata is a notorious HAB-forming species that has a cosmopolitan distribution covering global oceans (as reviewed in Tang and Gobler, 2012) [ 38 ]. Due to its wide geo-distribution, high capability to form cysts both in the laboratory and field, and increasing studies on the species as partly reviewed above, S. acuminata has been adopted as a representative species for life history studies on dinoflagellates [ 18 , 19 , 20 , 39 , 40 , 41 ]. In this study, using S. acuminata as a representative dinoflagellate again, we measured the transcriptional activity, cell/cyst viability, and intracellular ATP content of resting cysts under a range of conditions simulated to that possibly observed in the marine sediments.…”

Section: Introductionmentioning

confidence: 99%

Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements

Yue

Deng

et al. 2022

IJMS

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Similar to the seeds of higher plants, resting cysts, a non-motile, benthic, and dormant stage in the life history of many dinoflagellate species, play vital roles via germination in the seasonal dynamics and particularly the initiation of harmful algal blooms (HABs) of dinoflagellates. It is thus crucial for resting cysts to balance between the energetic catabolism for viability maintenance and the energy preservation for germination during their dormancy. Despite this importance, studies on how resting cysts of dinoflagellates accomplish energetic metabolism in marine sediment have been virtually absent. In this study, using the cosmopolitan HABs-causing species Scrippsiella acuminata as a representative, we measured the transcriptional activity of the most efficient pathway of the energy catabolism tricarboxylic acid (TCA) cycle, cell viability (via neutral red staining), and the cellular ATP content of resting cysts under a set of mock conditions in marine sediments (e.g., 4 °C, darkness, and anoxia) for a maximum period of one year. Based on the correlation analyses among the expression levels of genes, cyst viability, and ATP content, we revealed that the TCA cycle was still a crucial pathway of energetic catabolism for resting cysts under aerobic conditions, and its expression was elevated at higher temperatures, light irradiation, and the early stage of dormancy. Under anaerobic conditions, however, the TCA cycle pathway ceased expression in resting cysts, as also supported by ATP measurements. Our results have laid a cornerstone for the comprehensive revelation of the energetic metabolism and biochemical processes of dormancy of resting cysts in marine sediments.

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Allelopathy and underlying mechanism of Chlorella pyrenoidosa on the bloom-causing dinoflagellate Heterocapsa bohaiensis

Peng,

Li,

Zhou

et al. 2025

Algal Research

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The responses of Scrippsiella acuminata to the stresses of darkness: antioxidant activities and formation of pellicle cysts

Cited by 9 publications

References 48 publications

Effects of aeration and centrifugation conditions on omega-3 fatty acid production by the mixotrophic dinoflagellate Gymnodinium smaydae in a semi-continuous cultivation system on a pilot scale

Effects of aeration and centrifugation conditions on omega-3 fatty acid production by the mixotrophic dinoflagellate Gymnodinium smaydae in a semi-continuous cultivation system on a pilot scale

Characterizing the Status of Energetic Metabolism of Dinoflagellate Resting Cysts under Mock Conditions of Marine Sediments via Physiological and Transcriptional Measurements

Allelopathy and underlying mechanism of Chlorella pyrenoidosa on the bloom-causing dinoflagellate Heterocapsa bohaiensis

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