This study aimed to assess soil organic carbon (SOC) concentration and stock in mineral soils in cropland and grassland in Latvia, considering soil groups and texture classes. It covered 197 sites across Latvia (152 in cropland, 45 in grassland). Soil profile description and sampling (at depths of 0–10 cm, 10–20 cm, and 20–40 cm) were conducted between 2021 and 2023. Laboratory analyses included soil bulk density (SBD), total carbon (TC), total nitrogen (TN), carbonate content, pH, and extractable phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg). SOC stock was calculated, and correlations with other soil parameters were determined. In cropland sites, Arenosols and Stagnosols had the lowest SOC concentration and stock, while Gleysols and Phaeozems had the highest. In grassland sites, Retisols exhibited the lowest SOC concentration in the 0–20 cm layer, while Planosols had the highest SOC concentration in this layer. Conversely, in the 20–40 cm layer, Retisols showed the highest SOC concentration, while Gleysols had the lowest concentration. Regarding SOC stock in grassland sites, Planosols exhibited the highest values, while the lowest values were observed for Retisols and Umbrisols. Contrary to our hypothesis that grassland exhibits higher SOC stock than cropland, our results show the reverse for Phaeozems, the dominant WRB soil group in this study: a higher average SOC concentration and stock in cropland compared to grassland. However, very low occurrence of some soil groups and lack of some soil groups for grassland sites hinders the correct interpretation of these results, and further investigations are required in future studies.