The Results of «lb-Complex L» Immobilized Synbiotic Safety Study in Animals

Belova, Irina V.; Точилина, А. Г.; Соловьева, И. В.; Zhilenkova, O.G.; Melikhova, A.V.; Belyaeva, E.V.; Ermolina, G.B.; Boriskina, E.V.; Zhirnov, V.A.; Ivanova, T.P.

doi:10.17513/mjpfi.12113

Cited by 3 publications

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“…After the combined therapy, both in the control and the LB-complex L treated group, bifidobacteria were detected in 100% of patients [ 17 ]. However, in the main group, the isolation procedure resulted in 10 9 –10 10 CFU/g, while in control, the figure was 10 7 –10 8 CFU/g (p<0.05).…”

Section: Resultsmentioning

confidence: 99%

“…As a probiotic component of the diet therapy, we used our proprietary immobilized multistrain synbiotic LB-complex L (Certificate of State Registration — RU.77.99.88.003.E.002522.06.18) [ 17 ]. LB-complex L is recommended as a source of probiotic microorganisms ( bifidobacteria and lactobacilli ) and zeolites (enterosorbents) that increase the body’s non-specific resistance and have a detoxifying effect.…”

Section: Methodsmentioning

confidence: 99%

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Colon Microbiocenosis and Its Correction in Patients Receiving Programmed Hemodialysis

Belova¹,

Хрулев²,

Точилина³

et al. 2020

Sovrem Tehnol Med

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The aim of the investigation was to study the species composition of colon microbiocenosis in patients with chronic kidney disease receiving programmed hemodialysis treatment and to evaluate the efficacy of its correction using a new immobilized synbiotic. Materials and Methods. Samples of colon microbiota from 62 patients undergoing programmed hemodialysis were studied before and after a course of diet therapy that included probiotic components, in particular, the immobilized synbiotic LB-complex L. Isolation of microorganisms was carried out according to our original method; for bacteria identification, a MALDI-TOF Autoflex speed mass spectrometer (Bruker Daltonik, Germany) was used in the Biotyper program mode. The results were assessed using the criteria proposed by the authors and based on the OST 91500.11.0004-2003. The efficacy of the immobilized synbiotic was determined based on the clinical data, questionnaires, and bacteriological tests. Results. In patients receiving programmed hemodialysis (before the start of the diet therapy), chronic moderate inflammation and azotemia were found. Dysbiotic changes in microbiocenosis were revealed in all the examined patients; in the absence or suppression of lacto- and bifidoflora, the number and diversity of Bacteroides spp. , Clostridium spp. , Collinsella spp. , Eggerthella spp. and other bacteria increased, which was consistent with the theory of functional redundancy of gut microbiota. From the answers to the questionnaires, a decrease in the quality of life was found (up to 70 points out of 100) according to six of the eight scales used. After the combined therapy using the synbiotic LB-complex L in the study group, 56% of the examined patients showed their microbiocenosis restored to normal; no grade III dysbiosis was detected in any patient. There was a significant decrease in CRP and ESR in these patients and an improvement in the quality of life by criteria reflecting physical health. Conclusion. In patients receiving programmed hemodialysis, the addition of a probiotic component in the diet therapy restores the evolutionarily determined structure of the microbiocenosis, normalizes its functions, and leads to an overall improvement in health and quality of life.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Colon Microbiocenosis and Its Correction in Patients Receiving Programmed Hemodialysis

Belova¹,

Хрулев²,

Точилина³

et al. 2020

Sovrem Tehnol Med

Self Cite

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“…Microorganisms were identified on an autoflex speed time-of-flight MALDI-TOF mass spectrometer (Bruker Daltonik, Germany) using the Biotyper 4.1.80 RTC program. The author's immobilized multistrain synbiotic LB-complex L (SGR RU.77.99.88.003.E.002522.06.18) [56], recommended as a source of probiotic microorganisms (bifidobacteria and lactobacilli) and zeolites (enterosorbent), which increase the body's nonspecific resistance and have a detoxifying effect. Six strains that make up the synbiotic under study belong to species with a documented history of safe use and are approved for the production of medical immunobiological preparations.…”

Section: Methodsmentioning

confidence: 99%

Specific Cerebrovascular Risk Factors, Colon Microbiocenosis and Its Correction in Patients Receiving Long-Term Programmed Hemodialysis

Хрулев¹,

Belova²,

Соловьева³

et al. 2022

Multidisciplinary Experiences in Renal Replacement Therapy

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Introduction: The problem of acute and chronic cerebrovascular disorders in dialysis patients remains the most urgent. Risk factors for cerebrovascular diseases in CKD and dialysis patients can be conditionally divided into “traditional” (arterial hypertension, diabetes mellitus, hypercholesterolemia) and “specific” (associated with renal pathology and dialysis procedures). The spectrum of specific factors of cerebrovascular risk in patients with dialysis stage of the CKD includes specific dialysis factors that form during programmed HD, as well as impaired phosphorus-calcium metabolism and calcification of the arterial microvasculature, increased blood levels of β2-microglobulin, homocysteine, malondialdehyde and superoxide dismutase, a decrease in the level of nitric oxide (II) metabolites, development of nephrogenic anemia and dysfunction of blood cells, malnutrition and dietary features of patients with renal pathology, accumulation of uremic toxins and toxins of intestinal bacteria, etc. Opportunistic gut microorganisms can produce uremic toxins, which are associated with an increased risk of inflammation, increased oxidative stress, and a higher risk of cardiovascular disease (CVD). Description of the spectrum of risk factors for cerebrovascular pathology in dialysis patients and effective control over them seems to be an effective strategy aimed at increasing the duration and quality of life in patients receiving renal replacement therapy. The aim of the investigation was to study the species composition of colon microbiocenosis in patients with CKD receiving programmed HD treatment and to evaluate the effectiveness of its correction using a new immobilized synbiotic. Materials and methods: Samples of colon microbiota from 62 patients undergoing programmed hemodialysis were studied before and after a course of diet therapy that included probiotic components, in particular, the immobilized synbiotic LB-complex L. Isolation of microorganisms was carried out according to our original method; for bacteria identification, a MALDI-TOF Autoflex speed mass spectrometer (Bruker Daltonik, Germany) was used in the Biotyper program mode. The results were assessed using the criteria proposed by the authors and based on the OST 91500.11.0004-2003. The efficacy of the immobilized synbiotic was determined based on the clinical data, questionnaires, and bacteriological tests. Results: In patients receiving programmed hemodialysis (before the start of the diet therapy), chronic moderate inflammation and azotemia were found. Dysbiotic changes in microbiocenosis were revealed in all the examined patients; in the absence or suppression of lacto- and bifidoflora, the number and diversity of Bacteroides spp., Clostridium spp., Collinsella spp., Eggerthella spp. and other bacteria increased, which was consistent with the theory of functional redundancy of gut microbiota. From the answers to the questionnaires, a decrease in the quality of life was found (up to 70 points out of 100) according to six of the eight scales used. After the combined therapy using the synbiotic LB-complex L in the study group, 56% of the examined patients showed their microbiocenosis restored to normal; no grade III dysbiosis was detected in any patient. There was a significant decrease in CRP and ESR in these patients and an improvement in the quality of life by criteria reflecting physical health. Conclusion: Acute/chronic CVD in patients with CKD of the pre-dialysis and dialysis periods are the most frequent and formidable complications. The spectrum of “traditional” and “specific” CV risk factors in dialysis patients will be described in the chapter. Special attention will be paid to the intestinal microbiota and opportunistic intestinal microorganisms. The aim was to study the species composition of colon microbiocenosis in HD patients, and to evaluate the effectiveness of its correction using a new immobilized synbiotic. Materials and Methods. Samples of colon microbiota from 62 HD patients were studied before/after a course of diet therapy that included probiotic components, the immobilized synbiotic LB-complex L. MALDI-TOF Autoflex speed mass spectrometer was used in the Biotyper program mode. The efficacy of the immobilized synbiotic was determined based on the clinical data, questionnaires, and bacteriological tests. Results. Dysbiotic changes in microbiocenosis were revealed in all patients; in the absence/suppression of lacto-and bifidoflora, the number and diversity of Bacteroides spp.,Clostridium spp.,Collinsella spp.,Eggerthella spp. and other bacteria increased. After the combined therapy using the synbiotic LB-complex L in the study group, 56% of the examined patients showed their microbiocenosis restored to normal; no grade III dysbiosis was detected in any patient.

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Determination of the Toxicity and Sensitizing effect of a Therapeutic Probiotic agent against Intestinal and Concomitant human infections

Gavrilova,

Ratnikova,

Sadanov

et al. 2023

RJPT

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Studies were conducted on the toxicity and possible sensitizing effect of the lyophilizate of a therapeutic probiotic agent from the most active strains of lactic acid and propionic acid bacteria isolated from healthy people: L. plantarum 2v/A-6+L. brevis B-3/A-26+L. acidophilus 27w/60+P. shermanii 8. Based on the studies conducted to evaluate acute toxicity with an oral single administration of probiotic agent solutions (lyophilizate of lactic acid and propionic acid bacteria) at doses of 300, 2,000, 4,000, and 5,001mg/kg of weight, the toxicity class of the preparation under study has been defined as a non-toxic substance. In the study on laboratory mice of subchronic (subacute) toxicity of probiotic agent (lyophilizate of lactic acid and propionic acid bacteria)) in doses of 5.0×106, 10.0×106, and 15.0×106 CFU, it has been found that the preparation does not have a toxic effect on the body in case of 28-day use. Studies of the chronic toxicity of the probiotic agent (lyophilizate of lactic acid and propionic acid bacteria) in doses of 5.0×106, 10.0×106, and 15.0×106 CFU, in laboratory mice have shown that this preparation does not have a toxic effect on the body in case of prolonged 60-day use and does not cause structural changes in internal organs. It has been established that the therapeutic probiotic agent (lyophilizate of lactic acid and propionic acid bacteria) does not have a potential sensitizing (allergenic) effect on rat peritoneal mast cells.

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The Results of «lb-Complex L» Immobilized Synbiotic Safety Study in Animals

Cited by 3 publications

References 0 publications

Colon Microbiocenosis and Its Correction in Patients Receiving Programmed Hemodialysis

Colon Microbiocenosis and Its Correction in Patients Receiving Programmed Hemodialysis

Specific Cerebrovascular Risk Factors, Colon Microbiocenosis and Its Correction in Patients Receiving Long-Term Programmed Hemodialysis

Determination of the Toxicity and Sensitizing effect of a Therapeutic Probiotic agent against Intestinal and Concomitant human infections

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