The Reticulum-Associated Protein RTN1A Specifically Identifies Human Dendritic Cells

Gschwandtner, Maria; Kienzl, Philip; Tajpara, Poojabahen; Schuster, Christopher; Stipek, Gernot; Buchberger, Maria; Mildner, Michael; Mairhofer, Mario; Eppel, Wolfgang; Vierhapper, Martin; Pammer, Johannes; Koller, Rupert; Elbe‐Bürger, Adelheid; Tschachler, Erwin

doi:10.1016/j.jid.2018.01.002

Cited by 7 publications

(8 citation statements)

References 44 publications

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“…This finding suggested that also other dermal populations may have infiltrated the epidermis upon negative pressure and/or markers may have been upregulated on resident LCs. Confirming our previous observation in normal skin 34 , CD11c expression was undetectable on resident LCs in normal epidermis (Fig. 4G-J) and blister roof epidermis (Fig.…”

Section: Resultssupporting

confidence: 92%

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Re-epithelialization and immune cell behaviour in an ex vivo human skin model

Rakita

Nikolić

Mildner

et al. 2020

Sci Rep

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11,763

361

6,980

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A large body of literature is available on wound healing in humans. Nonetheless, a standardized ex vivo wound model without disruption of the dermal compartment has not been put forward with compelling justification. Here, we present a novel wound model based on application of negative pressure and its effects for epidermal regeneration and immune cell behaviour. Importantly, the basement membrane remained intact after blister roof removal and keratinocytes were absent in the wounded area. Upon six days of culture, the wound was covered with one to three-cell thick K14+Ki67+ keratinocyte layers, indicating that proliferation and migration were involved in wound closure. After eight to twelve days, a multi-layered epidermis was formed expressing epidermal differentiation markers (K10, filaggrin, DSG-1, CDSN). Investigations about immune cell-specific manners revealed more T cells in the blister roof epidermis compared to normal epidermis. We identified several cell populations in blister roof epidermis and suction blister fluid that are absent in normal epidermis which correlated with their decrease in the dermis, indicating a dermal efflux upon negative pressure. Together, our model recapitulates the main features of epithelial wound regeneration, and can be applied for testing wound healing therapies and investigating underlying mechanisms.

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Section: Resultssupporting

confidence: 92%

“…S3E-H), CD11c ( Fig. S3M-P) and RTN1A, a recently identified marker for cells of the DC lineage 34 ( Fig. 5B; Fig.…”

Section: Resultsmentioning

confidence: 81%

Re-epithelialization and immune cell behaviour in an ex vivo human skin model

Rakita

Nikolić

Mildner

et al. 2020

Sci Rep

Self Cite

11,763

361

6,980

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“…PBMCs and neutrophils (purity >95%) were isolated as described in Gschwandtner et al and Polak et al ( 32 , 33 ), and 2 × 10 5 cells in 30 μl RPMI 1640 medium were added onto the membrane of the migration system (ChemoTx; Neuro Probe, Gaithersburg, MD, USA). The lower compartment was filled with 300 μl of 100 ng/ml CXCL8 (neutrophils) or CXCL13 (PBMCs).…”

Section: Methodsmentioning

confidence: 99%

The cytokine environment influence on human skin‐derived T cells

et al. 2019

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Skin resident T cells provide immediate immunologic responses at their specific location and play a role in the pathogenesis of skin diseases such as psoriasis. Recently, IL-9–producing T cells were described as a major T-cell subtype present in the skin, but knowledge on the biology and in situ regulation of this T-cell subtype is scarce. Here, we investigated the cytokine influence on skin T cells with focus on IL-9–producing T cells because a better understanding of their biology may identify novel therapeutic approaches. Healthy human skin biopsies were cultured either in the presence of IL-2, IL-4, and TGF-β [T helper (T h )9–promoting condition (T h 9-PC)] or IL-2 and IL-15 [standard condition (SC)]. Paired analysis of enzymatically isolated skin T cells and emigrated T cells after 4 wk of skin culture showed significant alterations of T-cell phenotypes, cytokine production, and IL-9–producing T-cell frequency. RNA sequencing analysis revealed differentially regulated pathways and identified CXCL8 and CXCL13 as top up-regulated genes in T h 9-PC compared with SC. Functionally supernatant of stimulated skin-derived T cells, CXCL8 and CXCL13 increased neutrophil survival. We report that the cytokine environment alters skin-derived T-cell phenotype and functional properties.—Kienzl, P., Polacek, R., Reithofer, M., Reitermaier, R., Hagenbach, P., Tajpara, P., Vierhapper, M., Gschwandtner, M., Mildner, M. Jahn-Schmid, B., Elbe-Bürger, A. The cytokine environment influence on human skin–derived T cells.

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“…Next potential markers are CD207 [66], and CD209 (DC-SIGN), which is expressed on plasmacytoid DCs (pDCs) and moDCs and is greatly expressed on DCs in mucosal tissues. The expression of this last marker is increased after LPS-mediated DC maturation induction [67].…”

Section: The Immunophenotyping Of Modcsmentioning

confidence: 99%

In Laboratory Generation and Maturation of Human Monocyte-Derived Dendritic Cells for Cancer Immunotherapy

Mishra¹,

Bharadva²,

Joshi

et al. 2020

Int J App Pharm

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Dendritic cells (DCs) play a critical role in the regulation of adaptive immune responses, furthermore they act as a bridge between the innate and the adaptive immune systems they have been ideal candidates for cell-based immunotherapy of cancers and infections in humans. The first reported trial using DCs in 1995, since they have been used in trials all over the world for several of indications, including cancer and human immunodeficiency virus infection. Generally, for in vitro experiments or for DCs vaccination monocyte-derived dendritic cells (moDCs) were generated from purified monocytes that isolated from peripheral blood by density gradient centrifugation. A variety of methods can be used for enrichment of monocytes for generation of clinical-grade DCs. Herein we summarized up to date understanding of systems and inputs used in procedures to differentiate DCs from blood monocytes in vitro.

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The Reticulum-Associated Protein RTN1A Specifically Identifies Human Dendritic Cells

Cited by 7 publications

References 44 publications

Re-epithelialization and immune cell behaviour in an ex vivo human skin model

Re-epithelialization and immune cell behaviour in an ex vivo human skin model

The cytokine environment influence on human skin‐derived T cells

In Laboratory Generation and Maturation of Human Monocyte-Derived Dendritic Cells for Cancer Immunotherapy

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