The retromer protein ZmVPS29 regulates maize kernel morphology likely through an auxin‐dependent process(es)

Chen, Lin; Li, Yongxiang; Li, Chunhui; Shi, Yunsu; Song, Yi; Zhang, Dengfeng; Wang, Haiyang; Liu, Yu; Wang, Tianyu

doi:10.1111/pbi.13267

Cited by 30 publications

(14 citation statements)

References 41 publications

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“…Plant hormones are one of the most important factors affecting the growth and development of grains [ 23 , 24 ]. Cytokinin and brassinolide play a vital role in regulating seed size, auxin, ABA, and gibberellin have regulatory effects on seed development to a certain extent [ 25 ]. Through transcriptomics analysis, we found that the signal transduction pathway has a notable influence on grain size.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of the maize inbred line Chang7-2 and a large-grain mutant tc19

et al. 2022

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Backgrounds Grain size is a key factor in crop yield that gradually develops after pollination. However, few studies have reported gene expression patterns in maize grain development using large-grain mutants. To investigate the developmental mechanisms of grain size, we analyzed a large-grain mutant, named tc19, at the morphological and transcriptome level at five stages corresponding to days after pollination (DAP). Results After maturation, the grain length, width, and thickness in tc19 were greater than that in Chang7-2 (control) and increased by 3.57, 8.80, and 3.88%, respectively. Further analysis showed that grain width and 100-kernel weight in tc19 was lower than in Chang7-2 at 14 and 21 DAP, but greater than that in Chang7-2 at 28 DAP, indicating that 21 to 28 DAP was the critical stage for kernel width and weight development. For all five stages, the concentrations of auxin and brassinosteroids were significantly higher in tc19 than in Chang7-2. Gibberellin was higher at 7, 14, and 21 DAP, and cytokinin was higher at 21 and 35 DAP, in tc19 than in Chang7-2. Through transcriptome analysis at 14, 21, and 28 DAP, we identified 2987, 2647 and 3209 differentially expressed genes (DEGs) between tc19 and Chang7-2. By using KEGG analysis, 556, 500 and 633 DEGs at 14, 21 and 28 DAP were pathway annotated, respectively, 77 of them are related to plant hormone signal transduction pathway. ARF3, AO2, DWF4 and XTH are higher expressed in tc19 than that in Chang7-2. Conclusions We found some DEGs in maize grain development by using Chang7-2 and a large-grain mutant tc19. These DEGs have potential application value in improving maize performance.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of the maize inbred line Chang7-2 and a large-grain mutant tc19

et al. 2022

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“…The retromer components VPS35A and VPS29 are essential for normal prevacuolar compartment morphology and normal trafficking of plasma membrane proteins in plants (Nodzyński et al 2013). A recent study showed that ZmVPS29 is involved in auxin accumulation during early kernel development in maize (Chen et al 2020). An evolutionarily conserved VPS26 protein (VPS26C; At1G48550) functions in a complex with VPS35A and VPS29, which are necessary for root hair growth in Arabidopsis (Jha et al 2018).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of the NRAMP Family in Melon

Jin¹

2021

IJAB

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The NRAMPs (natural resistance-associated macrophage proteins) are evolutionarily conserved integral membrane proteins that transport a broad range of metal ions. A comprehensive analysis of NRAMP family genes has not been reported for melon (Cucumis melo). In this study, six CmNRAMP genes were identified from the melon genome. Analyses of gene structure, phylogeny, chromosome location and conserved motifs revealed two groups of CmNRAMPs: one group contained only CmNRAMP5, which had 12 conserved motifs like NRAMPs in Arabidopsis thaliana and rice (Oryza sativa). The other group contained five CmNRAMPs and all had shorter amino acid sequences compared with CmNRAMP5. Expression analyses suggested that CmNRAMP genes are expressed differently in response to treatments with different plant hormones, abiotic factors, and metals (iron, copper, and manganese). The most responsive gene was CmNRAMP5, which showed significant changes in its transcript levels in response to abscisic acid (ABA), indole acetic acid, cytokinin, methyl jasmonate, brassinosteroid, Fe, and Cu. The least responsive gene was CmNARMP6, which was only significantly responsive to ABA, Fe, and Mn. These results indicate that CmNRAMP genes function in numerous tissues and at different developmental stages. A protein–protein interaction analysis indicated that CmNRAMPs interact with proteins involved in transmembrane transport, superoxide dismutase activity, plant hormone signal transduction, signal transduction by protein phosphorylation, and nicotianamine synthase activity. This study provides valuable insights into the potential function of CmNRAMP genes and their encoded products. © 2021 Friends Science Publishers

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“…Many genes related to plant hormones have been con rmed to play key roles in regulating plant kernel development [20]. In recent years, it has been discovered that cytokinin and brassinolide play a vital role in regulating seed size, and in addition, auxin, ABA, and gibberellin have regulatory effects on seed development to a certain extent [21]. In this study, we detected changes in IAA, GA3, CTK, and BR during grain development in Chang 7 − 2 and TC19.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of the Maize Mutant TC19 Identifies Genes Regulating the Development of Grain Size Through the IAA and BR Pathways

Zhang

Jiao

Jun

et al. 2021

Preprint

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Backgrounds: Grain size is a key factor in crop yield that gradually develops after pollination. However, few studies have reported gene expression patterns in maize grain development using mutants. To investigate the developmental mechanisms of grain size, we analyzed a large-grain mutant, named TC19, at the morphological and transcriptome level at five stages corresponding to days after pollination (DAP).Results: After maturation, the grain length, width, and thickness in TC19 were greater than that in Chang 7-2 (control) and increased by 3.57%, 8.80%, and 3.88%, respectively. Further analysis showed that grain width in TC19 was lower than in Chang 7-2 at 7, 14, and 21 DAP, but greater than that in Chang 7-2 at 28 and 35 DAP, indicating that 21 to 28 DAP was the critical stage for kernel width development. For all five stages, the concentrations of indole-3-acetic acid and brassinosteroids were significantly higher in TC19 than in Chang 7-2. Gibberellin was higher at 7, 14, and 21 DAP, and cytokinin was higher at 21 and 35 DAP, in TC19 than in Chang 7-2. Through transcriptome analysis at 14, 21, and 28 DAP, we identified 2987, 2647, and 3209 differentially expressed genes (DEGs) between TC19 and Chang 7-2. Gene Ontology analysis indicated that most of the grain size–related genes corresponded to three aspects, including cell components, molecular functions, and biological processes. The Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that 77 DEGs were enriched in the plant hormone signal transduction pathway. We further analyzed several highly expressed candidate genes, including AO2, ARF3, and IAA15, which are involved in the synthesis of IAA; and DWF4 and XTH, which are involved in the synthesis of BR.Conclusions: Our results elucidated the mechanisms of grain size development at the grain-filling stage and have potential application in maize breeding.

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The retromer protein ZmVPS29 regulates maize kernel morphology likely through an auxin‐dependent process(es)

Cited by 30 publications

References 41 publications

Transcriptomic analysis of the maize inbred line Chang7-2 and a large-grain mutant tc19

Transcriptomic analysis of the maize inbred line Chang7-2 and a large-grain mutant tc19

Genome-Wide Identification and Expression Analysis of the NRAMP Family in Melon

Transcriptomic Analysis of the Maize Mutant TC19 Identifies Genes Regulating the Development of Grain Size Through the IAA and BR Pathways

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