The reversal of serological reactions in patients with pulmonary and pleural aspergillosis after treatment

Halweg, H; Ciszek, J; Krakówka, P

doi:10.1016/s0041-3879(68)80021-2

Cited by 12 publications

(1 citation statement)

References 4 publications

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“…This hypothesis can be tested by assessing the progression of the serological curve of anti- Aspergillus IgG, as currently there is a kit available for this determination, which will be performed soon. The decrease in serum antibody levels, determined by DID test, after the introduction of an effective treatment, was also observed by other authors [ 5 , 43 , 54 , 55 ]. Thus, based on the results available, the follow-up of patients must be performed using DID.…”

Section: Discussionsupporting

confidence: 80%

Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the Diagnosis and Follow-Up of Patients with Chronic Pulmonary Aspergillosis

et al. 2015

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The diagnosis of chronic pulmonary aspergillosis (CPA) depends on the radiologic image and the identification of specific antibodies. The present study aimed to evaluate accuracy parameters of enzyme-linked immunosorbent assay (ELISA) and of the determination of serum galactomannan level in the diagnosis of patients with CPA, comparing these results with the double agar gel immunodiffusion (DID) test. In addition, the prevalence of cross-reactivity and the serological progression after treatment were evaluated by comparing DID and ELISA. Six study groups were formed: G1: 22 patients with CPA, 17 of whom had Aspergillus fungus ball, one chronic cavitary pulmonary aspergillosis (CCPA) and four chronic fibrosing pulmonary aspergillosis (CFPA); G2: 28 patients with pulmonary tuberculosis (TB); G3: 23 patients with histoplasmosis (HST); G4: 50 patients with paracoccidioidomycosis (PCM); G5: 20 patients with cryptococcosis (CRC); and G6: 200 healthy controls. Serum antibodies were measured by DID and ELISA, with two antigen preparations—Aspergillus fumigatus (DID1, ELISA1) and a pool of A. fumigatus, A. flavus and A. niger antigens (DID2, ELISA2). The Platélia Aspergillus Enzyme Immunoassay (EIA) kit was used to measure galactomannan. The cut-off points of ELISA were determined for each antigen preparation and for the 95% and 99% confidence intervals. Despite the low sensitivity, DID was the technique of choice due to its specificity, positive and negative predictive values and positive likelihood ratio–especially with the antigen pool and due to the low frequency of cross-reactivity. ELISA1 and a 0.090 cut-off showed high sensitivity, specificity and negative predictive value, but a high frequency of cross-reactivity with CRC. The best degree of agreement was observed between ELISA1 and ELISA2. The detection of serum galactomannan showed high sensitivity, comparable to ELISA2. The immunodiffusion test showed an excellent relationship with the progression after treatment, which made it the reaction of choice for patient follow-up.

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Section: Discussionsupporting

confidence: 80%

Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the Diagnosis and Follow-Up of Patients with Chronic Pulmonary Aspergillosis

et al. 2015

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Das Aspergillom der Lunge

Brändli

1984

Ergebnisse Der Inneren Medizin Und Kinderheilkunde/Advances in Internal Medicine and Pediatrics

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Serology of Aspergillus granuloma

Talwar¹,

Sharma²,

Sehgal

et al. 1982

Mycopathologia

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A total of 21 cases of granuloma caused by Aspergillus species were encountered during the period 1972-79. The organs involved were nasal and paranasal sinuses, brain, orbit, subcutaneous tissue of check, lungs and endocardial valve in the decreasing order of their frequency. Aspergillus flavus was the main etiological agent. Immunodiffusion tests with various Aspergillus species as antigen showed a positively of 17 out of 18 (94.4 per cent) cases. The role of precipitins in diagnosis and prognosis of the disease has been discussed.

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The reversal of serological reactions in patients with pulmonary and pleural aspergillosis after treatment

Cited by 12 publications

References 4 publications

Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the Diagnosis and Follow-Up of Patients with Chronic Pulmonary Aspergillosis

Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the Diagnosis and Follow-Up of Patients with Chronic Pulmonary Aspergillosis

Das Aspergillom der Lunge

Serology of Aspergillus granuloma

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