The rhabdoviruses: Biodiversity, phylogenetics, and evolution☆

Kuzmin, Ivan V.; Novella, Isabel S.; Dietzgen, Ralf G.; Padhi, Abinash; Rupprecht, Charles E.

doi:10.1016/j.meegid.2009.02.005

Cited by 164 publications

(130 citation statements)

References 139 publications

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“…Rhabdoviruses are ubiquitous in nature and have a broad host range, including invertebrates, vertebrates, and plants (30). Over 200 rhabdoviruses have been identified (31), and a majority of the plant rhabdoviruses and some vertebrate rhabdoviruses use an insect vector for transmission.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Galvin

Glasner

et al. 2014

J Virol

127

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The Sf9 cell line, derived from Spodoptera frugiperda, is used as a cell substrate for biological products, and no viruses have been reported in this cell line after extensive testing. We used degenerate PCR assays and massively parallel sequencing ( IMPORTANCEThe Spodoptera frugiperda Sf9 cell line is used as a cell substrate for the development and manufacture of biological products. Extensive testing has not previously identified any viruses in this cell line. This paper reports on the identification and characterization of a novel rhabdovirus in Sf9 cells. This was accomplished through the use of next-generation sequencing platforms, de novo assembly tools, and extensive bioinformatics analysis. Rhabdovirus identification was further confirmed by transmission electron microscopy. Infectivity studies showed the lack of replication of Sf-rhabdovirus in human cell lines. The overall study highlights the use of a combinatorial testing approach including conventional methods and new technologies for evaluation of cell lines for unexpected viruses and use of comprehensive bioinformatics strategies for obtaining confident next-generation sequencing results.

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Section: Discussionmentioning

confidence: 99%

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Galvin

Glasner

et al. 2014

J Virol

127

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“…The L polymerase gene of rhabdoviruses has proven to be a useful region to infer phylogenetic relationships among this diverse family of viruses that infect plants, fish and mammals (Bouhry et al 2005;Kuzmin et al 2009). This was also the case for DYVV which appears most closely related to SYNV among the plant rhabdoviruses where sequence information is available.…”

Section: Discussionmentioning

confidence: 99%

Partial polymerase gene sequence, phylogeny and RT-PCR diagnostic assay for Datura yellow vein nucleorhabdovirus

Dietzgen

Tan

Yong

et al. 2012

Australasian Plant Dis. Notes

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Partial (c. 0.25 and 1.1 kb) nucleotide sequences of the L polymerase gene of Datura yellow vein virus (DYVV) were obtained using two sets of degenerate oligonucleotide primers. This is the first sequence information for this virus. Phylogenetic analysis of the conserved L gene sequence with those of other plant rhabdoviruses showed that DYVV is most closely related to Sonchus yellow net virus and confirmed its taxonomic placement in the genus Nucleorhabdovirus. DYVV strains isolated from Datura stramonium and Thungergia alata, respectively, were 97.2 % identical in nucleotide sequence across a 251 nt region of the L gene. DYVV-specific primers were designed and a diagnostic RT-PCR assay developed and validated.

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“…The family Rhabdoviridae (order Mononegavirales) is composed of a large and versatile group of viruses that are ubiquitous in nature (Kuzmin et al, 2009). The family consists of 11 genera as well as several viruses that have not yet been assigned to a genus (http://www.…”

Section: Introductionmentioning

confidence: 99%

“…Virions have a distinctive bullet or cone-shaped morphology or appear bacilliform. Rhabdoviruses have broad host ranges that include humans and other terrestrial mammals, birds, reptiles, fish, insects and plants (Hoffmann et al, 2005;Kuzmin et al, 2009;Mann & Dietzgen, 2014). Many rhabdoviruses are transmitted to vertebrate and plant hosts by insect vectors in which they replicate (Hogenhout et al, 2003;Ammar et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Merida virus, a putative novel rhabdovirus discovered in Culex and Ochlerotatus spp. mosquitoes in the Yucatan Peninsula of Mexico

Charles

Firth

Loroño-Pino

et al. 2016

Journal of General Virology

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Sequences corresponding to a putative, novel rhabdovirus [designated Merida virus (MERDV)]were initially detected in a pool of Culex quinquefasciatus collected in the Yucatan Peninsula of Mexico. The entire genome was sequenced, revealing 11 798 nt and five major ORFs, which encode the nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G) and RNA-dependent RNA polymerase (L). The deduced amino acid sequences of the N, G and L proteins have no more than 24, 38 and 43 % identity, respectively, to the corresponding sequences of all other known rhabdoviruses, whereas those of the P and M proteins have no significant identity with any sequences in GenBank and their identity is only suggested based on their genome position. Using specific reverse transcription-PCR assays established from the genome sequence, 27 571 C. quinquefasciatus which had been sorted in 728 pools were screened to assess the prevalence of MERDV in nature and 25 pools were found positive. The minimal infection rate (calculated as the number of positive mosquito pools per 1000 mosquitoes tested) was 0.9, and similar for both females and males. Screening another 140 pools of 5484 mosquitoes belonging to four other genera identified positive pools of Ochlerotatus spp. mosquitoes, indicating that the host range is not restricted to C. quinquefasciatus. Attempts to isolate MERDV in C6/36 and Vero cells were unsuccessful. In summary, we provide evidence that a previously undescribed rhabdovirus occurs in mosquitoes in Mexico.

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The rhabdoviruses: Biodiversity, phylogenetics, and evolution☆

Cited by 164 publications

References 139 publications

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Identification of a Novel Rhabdovirus in Spodoptera frugiperda Cell Lines

Partial polymerase gene sequence, phylogeny and RT-PCR diagnostic assay for Datura yellow vein nucleorhabdovirus

Merida virus, a putative novel rhabdovirus discovered in Culex and Ochlerotatus spp. mosquitoes in the Yucatan Peninsula of Mexico

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