The ribonucleases J1 and J2 are essential for growth and have independent roles in mRNA decay in <i>Streptococcus pyogenes</i>

Bugrysheva, Julia V.; Scott, June R.

doi:10.1111/j.1365-2958.2009.07012.x

Cited by 64 publications

(79 citation statements)

References 36 publications

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“…S2A). This processing is probably carried out by RNases that were not included in this study, for instance RNases J1 or J2, which are essential for S. pyogenes survival [31]. We also cannot exclude that more than one RNase is capable of cleaving at this specific position ( i.e .…”

Section: Resultsmentioning

confidence: 99%

“…secondary structures) in the mRNA 5′ UTRs [42]. However, other unidentified requirements, apart from the 5′ UTR, are also needed to discriminate the different sets of mRNAs to allow differential stability of virulence-related mRNAs [31]. RNase Y is involved in the regulation of virulence in S. pyogenes , but to date no regulatory mechanisms have been elucidated.…”

Section: Discussionmentioning

confidence: 99%

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RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B

et al. 2018

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Endoribonuclease Y (RNase Y) is a crucial regulator of virulence in Gram-positive bacteria. In the human pathogen Streptococcus pyogenes, RNase Y is required for the expression of the major secreted virulence factor streptococcal pyrogenic exotoxin B (SpeB), but the mechanism involved in this regulation remains elusive. Here, we demonstrate that the 5′ untranslated region of speB mRNA is processed by several RNases including RNase Y. In particular, we identify two RNase Y cleavage sites located downstream of a guanosine (G) residue. To assess whether this nucleotide is required for RNase Y activity in vivo, we mutated it and demonstrate that the presence of this G residue is essential for the processing of the speB mRNA 5′ UTR by RNase Y. Although RNase Y directly targets and processes speB, we show that RNase Y-mediated regulation of speB expression occurs primarily at the transcriptional level and independently of the processing in the speB mRNA 5′ UTR. To conclude, we demonstrate for the first time that RNase Y processing of an mRNA target requires the presence of a G. We also provide new insights on the speB 5′ UTR and on the role of RNase Y in speB regulation.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B

et al. 2018

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“…4 The group A Streptococci (GAS) also have two RNase J orthologs that play a role in mRNA turnover and, interestingly, both are essential. 28 A single non-essential RNase J ortholog has been identified in Sinorhizobium meliloti and has been shown to play a role in the maturation of the 5' end of 16S rRNA and both 23S rRNA fragments present in this organism. 29 This would suggest that rRNA maturation is not the essential function of RNase J1.…”

Section: Role Of Rnase J In Other Organismsmentioning

confidence: 99%

What is the role of RNase J in mRNA turnover?

Condon¹

2010

RNA Biology

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T he discovery of the paralogous ribonucleases J1 and J2 has been a major advance in the study of RNA maturation and decay in Bacillus subtilis and related organisms. RNase J1 was the first bacterial enzyme shown to possess 5'-to-3' exoribonuclease activity, reversing a dogma that suggested this type of activity was unique to eukaryotic mRNA decay. RNase J1 and J2 form a complex that also has endonuclease activity and these enzymes have been shown to play a key role in the turnover and maturation of many RNAs in B. subtilis. Here, I describe recent progress in our understanding of the role of these enzymes in RNA metabolism in this organism and argue that the 5'-to-3' exoribonuclease activity may be the more important of the complex's two modes of action.

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“…The group A Streptococci (GAS) also have two RNase J orthologs and, interestingly, both are essential (30). In this organism, differential mRNA stability is an important mechanism for the regulation of virulence factors.…”

Section: Global Effects On Mrna Metabolismmentioning

confidence: 99%

“…The degradation of both classes of mRNAs is affected in RNase J1 and J2 conditional mutants. It has been suggested that degradation of class II transcripts only occurs once RNases J1 and J2 are released from their primary task, degrading preferred class I mRNAs (30).…”

Section: Global Effects On Mrna Metabolismmentioning

confidence: 99%

mRNA degradation and maturation in prokaryotes: the global players

Laalami

Putzer

2011

BioMolecular Concepts

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The degradation of messenger RNA is of universal importance for controlling gene expression. It directly affects protein synthesis by modulating the amount of mRNA available for translation. Regulation of mRNA decay provides an efficient means to produce just the proteins needed and to rapidly alter patterns of protein synthesis. In bacteria, the half-lives of individual mRNAs can differ by as much as two orders of magnitude, ranging from seconds to an hour. Most of what we know today about the diverse mechanisms of mRNA decay and maturation in prokaryotes comes from studies of the two model organisms Escherichia coli and Bacillus subtilis. Their evolutionary distance provided a large picture of potential pathways and enzymes involved in mRNA turnover. Among them are three ribonucleases, two of which have been discovered only recently, which have a truly general role in the initiating events of mRNA degradation: RNase E, RNase J and RNase Y. Their enzymatic characteristics probably determine the strategies of mRNA metabolism in the organism in which they are present. These ribonucleases are coded, alone or in various combinations, in all prokaryotic genomes, thus reflecting how mRNA turnover has been adapted to different ecological niches throughout evolution.

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The ribonucleases J1 and J2 are essential for growth and have independent roles in mRNA decay in Streptococcus pyogenes

Cited by 64 publications

References 36 publications

RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B

RNase Y-mediated regulation of the streptococcal pyrogenic exotoxin B

What is the role of RNase J in mRNA turnover?

mRNA degradation and maturation in prokaryotes: the global players

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