The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Terpstra, Peter; Holtrop, Marijke; Kroon, A.M.

doi:10.1016/0005-2787(77)90178-2

Cited by 12 publications

(2 citation statements)

References 25 publications

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“…After chilling to 0°C 1/zl of 0.1 M Na2SO3 and 50 #1 ammonium acetate (pH 9.3) were added and the incubation was continued for 20 min at 70°C. The 12SI-labelled RNA was purified by the procedure of Prensky et al [11], slightly modified as described elsewhere [9,12].…”

Section: Iodination Of the Rnasmentioning

confidence: 99%

The restriction fragment map of rat-liver mitochondrial DNA: A reconsideration

Kroon

Pèpe

Bakker

et al. 1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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1. Rat-liver mitochondrial DNA (mtDNA) contains at least 8 cleavage sites for the restriction endonuclease Eco RI, 6 for the restriction endonuclease Hind III, 2 for the restriction endonuclease Barn HI and 11 for the restriction endonuclease Hap II. 2. The physical map of the restriction fragments of Eco RI, Hind III, Barn HI and Hap II is constructed on the basis of: (a) the analysis of partially restricted fragments; (b) analysis of the double digests of total mtDNA; (c) the digestion of isolated restriction fragments with other restriction endonucleases; (d) the identification of fragments of complete single and double digestions and of partially digested fragments containing the base sequences complementary to the 12-S and 16~S RNAs of rat-liver mitochondrial ribosomes. 3. The genes for the ribosomal RNAs are shown to be closely linked. This result differs from data previously reported (Saccone, C., Pepe, G., Cantatore, P., Terpstra, P. and Kroon, A.M. (1976) in The Genetic Function of Mitochondrial DNA, pp. 27-36, Elsevier/North-Holland Biomedical Press, Amsterdam). 4. The origin of replication (D-loop) is localized in the vicinity of the small ribosomal RNA gene and the direction of replication is distant from this gene. 5. The mitochondrial tRNA genes are scattered over the genome as in other animal mtDNAs. The approximate minimal number of tRNA genes is 16-20. 6. We concluded previously that the Eco RI restriction fragments A and D are not adjacent and failed to show the overlap of the 16 S rRNA gene for the Eco RI fragment D and Hind III fragment A. This misinterpretation was due to the fact that the two smallest Eco RI fragments could not be detected with the Postal addresses: a Bloemsingel 10, Groningen; b Zernikelaan,

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Section: Iodination Of the Rnasmentioning

confidence: 99%

The restriction fragment map of rat-liver mitochondrial DNA: A reconsideration

Kroon

Pèpe

Bakker

et al. 1977

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein

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“…The arrangement and transcription of the ribosomal genes in spinach chloroplasts is thus similar to that in mutants of E. coli lacking RNase 111 in which 16-S, 23-S and 5-S rRNAs are transcribed as a single unit in the order given [33]. Similarly the mitochondrial DNAs of rat liver and Neurosporu crassa are known to contain adjacent genes for the RNAs of the large and small ribosomal subunits [34,35]. In the case of N .…”

Section: The Arrangement Transcription and Processing Of' Spinach Chmentioning

confidence: 80%

The Synthesis of Chloroplast High-Molecular-Weight Ribosomal Ribonucleic Acid in Spinach

Hartley

Head

1979

European Journal of Biochemistry

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have located the positions of the ribosomal genes on the restriction endonuclease map of Zea mays chloroplast DNA. The two rDNA units were shown to be separated from each other by 18 500 base pairs in one direction and by 106100 base pairs in the other direction and to have an inverted orientation with respect to each other. Each unit contains one sequence for the 16-S, 23-S and 5-S rRNAs in the order given, the 16-S and 23-S sequences in each unit being separated by a 2100-base-pairs spacer. A similar arrangement has recently been reported [6] for the ribosomal genes in spinach chloroplast DNA.Although the precise pathway of rRNA synthesis in chloroplasts is not known, studies on the kinetics of labelling in vivo of chloroplast rRNAs in both algae and higher plants suggest that the mature rRNAs are synthesised via precursors of 10 -20 % higher molecular -weight [7-101. In spinach, for example, Ahhreviurions. Hepes, 4-(2-hydroxyethyl-l-piperazineethanesulphonic acid ; Mes, 2(N-morpholino)ethanesulphonic acid; tricine, N-[tris(hydroxymethyl)]methylglycine; NaCl/Cit, 0.15 M NaCI, 0.015 M trisodium citrate.

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Mitochondrial DNA from Podospora anserina

1979

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Mitochondrial (Mt) DNA from mitochondrial mutants of race s Podospora anserina and from senescent cultures of races s and A was examined. In mutants, we observed that fewer full length circles (31 mu) were present; instead, smaller circles characteristic for each mutant studied were found. Eco R1 digestion of these mutant MtDNAs indicated that in certain mutants, although specific fragments were absent, the total molecular weight of the fragments was not much different than wild-type. The properties of senescent MtDNA was strikingly different from either wild-type or mutant Mt DNA. First, a multimeric set of circular DNA was observed for both race s and A, with a monomeric repeat size of 0.89 mu. These circles ranged in size from 0.89 mu to greater than 20 mu; only one molecule out of some 200 molecules was thought to be of full length (31 mu). Density gradient analysis showed that there were two density species: a majority were at the same density as wild-type (1.694 g/cm3) and a second at 1.699 g/cm3. Most of the circular molecules from MtDNA isolated by either total DNA extraction or by extraction of DNA from isolated mitochondria were contained in the heavy DNA fraction. Eco R1 enzymatic digestion indicated that the light DNA had several fragments (amounting to about 23 x 10(6) daltons) missing, compared with young, wild-type MtDNA. Heavy senescent MtDNA was not cleaved by Eco R1. Analysis with Hae III restriction endonuclease showed also that light senescent MtDNA was missing certain fragments. Heavy MtDNA of average size 20 x 10(6) daltons, yielded only one fragment, 2,500 bp long, by digestion with Hae III restriction endonuclease. Digestion of heavy DNA with Alu I enzyme yielded 10 fragments totalling 2,570 bp. By three criteria, electron-microscopy, Eco R1 and Hae digestion, we conclude that the heavy MtDNA isolated from senescent cultures of Podospora anserina consisted of a monomeric tandemly repeating subunit of about 2,600 bp length. These results on the properties of senescent MtDNA are discussed with regard to the published properties of the rho- mutation in the yeast, S. cerevisiae.

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The ribosomal RNA genes on Neurospora crassa mitochrondrial DNA are adjacent

Cited by 12 publications

References 25 publications

The restriction fragment map of rat-liver mitochondrial DNA: A reconsideration

The restriction fragment map of rat-liver mitochondrial DNA: A reconsideration

The Synthesis of Chloroplast High-Molecular-Weight Ribosomal Ribonucleic Acid in Spinach

Mitochondrial DNA from Podospora anserina

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