The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease

Bu, Zhigang; Li, Wei; Liu, Xiaoli; Liu, Ying; Gao, Yan; Pei, Gang; Zhuo, Rui; Cui, Kunpeng; Qin, Ziwei; Zheng, Heping; Wang, Jie; Yang, Yue; Su, Pin; Cao, Meiting; Xiong, Xianqiu; Liu, Xuanming; Zhu, Yonghua

doi:10.1128/spectrum.04824-22

Cited by 7 publications

(3 citation statements)

References 77 publications

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“…1e ). In our previous study, we also reported that OsiSh-2 can trigger rice immune responses by secreting α-Mannosidase ShAM1 to digest the rice cell wall and release damage-associated molecular patterns, and cell death was also observed at 24 hpt 51 . In addition, we revealed that appropriate ferroptosis was an essential factor for OsiSh-2 colonization and shaping the mutual symbiont.…”

Section: Discussionmentioning

confidence: 87%

“…This induction of an immune response for indirect disease resistance is not the only contribution of ferroptosis. Our recent study showed that applying ShAM1, an α-mannosidase purified from OsiSh-2 culture supernatants, can trigger rice immune responses and improve blast resistance with a 45.9% reduction in blast disease lesion length and a 67% reduction in fungal biomass 51 . Therefore, we considered that the assistance of OsiSh-2 in disease resistance in host rice depends on multiple mechanisms, and ferroptosis is one of them.…”

Section: Discussionmentioning

confidence: 99%

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Ferroptosis induction in host rice by endophyte OsiSh-2 is necessary for mutualism and disease resistance in symbiosis

Xiong,

Zeng,

Ning

et al. 2024

Nat Commun

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Ferroptosis is an iron-dependent cell death that was discovered recently. For beneficial microbes to establish mutualistic relationships with hosts, precisely controlled cell death in plant cells is necessary. However, whether ferroptosis is involved in the endophyte‒plant system is poorly understood. Here, we reported that endophytic Streptomyces hygroscopicus OsiSh-2, which established a sophisticated and beneficial interaction with host rice plants, caused ferroptotic cell death in rice characterized by ferroptosis- and immune-related markers. Treatments with ferroptosis inhibitors and inducers, different doses of OsiSh-2, and the siderophore synthesis-deficient mutant ΔcchH revealed that only moderate ferroptosis induced by endophytes is essential for the establishment of an optimal symbiont to enhance plant growth. Additionally, ferroptosis involved in a defence-primed state in rice, which contributed to improved resistance against rice blast disease. Overall, our study provides new insights into the mechanisms of endophyte‒plant interactions mediated by ferroptosis and suggests new directions for crop yield promotion.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Ferroptosis induction in host rice by endophyte OsiSh-2 is necessary for mutualism and disease resistance in symbiosis

Xiong,

Zeng,

Ning

et al. 2024

Nat Commun

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“…Plants may actively recruit microbial communities that can antagonize pathogenic microbes early during their growth, and the shaping of these microbial communities may be affected by host genes (see Section 3). The activation of host immune responses by endophytes can enhance the host's resistance to pathogens [170,175]. When external pathogens invade plants, there are changes in the microbial communities in different parts of the plant.…”

Section: The Endophyte Functions As An Extension Of the Plant's Immun...mentioning

confidence: 99%

Current Advances in the Functional Diversity and Mechanisms Underlying Endophyte–Plant Interactions

Zhao,

Onyino,

Gao

2024

Microorganisms

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Plant phenotype is a complex entity largely controlled by the genotype and various environmental factors. Importantly, co-evolution has allowed plants to coexist with the biotic factors in their surroundings. Recently, plant endophytes as an external plant phenotype, forming part of the complex plethora of the plant microbial assemblage, have gained immense attention from plant scientists. Functionally, endophytes impact the plant in many ways, including increasing nutrient availability, enhancing the ability of plants to cope with both abiotic and biotic stress, and enhancing the accumulation of important plant secondary metabolites. The current state of research has been devoted to evaluating the phenotypic impacts of endophytes on host plants, including their direct influence on plant metabolite accumulation and stress response. However, there is a knowledge gap in how genetic factors influence the interaction of endophytes with host plants, pathogens, and other plant microbial communities, eventually controlling the extended microbial plant phenotype. This review will summarize how host genetic factors can impact the abundance and functional diversity of the endophytic microbial community, how endophytes influence host gene expression, and the host–endophyte–pathogen disease triangle. This information will provide novel insights into how breeders could specifically target the plant–endophyte extended phenotype for crop improvement.

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The new CFEM protein CgCsa required for Fe3+ homeostasis regulates the growth, development, and pathogenicity of Colletotrichum gloeosporioides

Liu,

Bu,

Zhang

et al. 2024

International Journal of Biological Macromolecules

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The Rice Endophyte-Derived α-Mannosidase ShAM1 Degrades Host Cell Walls To Activate DAMP-Triggered Immunity against Disease

Abstract: The specific biological niche inside host plants allows endophytes to regulate plant disease resistance effectively. However, there have been few reports on the role of active metabolites from endophytes in inducing host disease resistance.

Cited by 7 publications

References 77 publications

Ferroptosis induction in host rice by endophyte OsiSh-2 is necessary for mutualism and disease resistance in symbiosis

Ferroptosis induction in host rice by endophyte OsiSh-2 is necessary for mutualism and disease resistance in symbiosis

Current Advances in the Functional Diversity and Mechanisms Underlying Endophyte–Plant Interactions

The new CFEM protein CgCsa required for Fe3+ homeostasis regulates the growth, development, and pathogenicity of Colletotrichum gloeosporioides

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