The Right Dorsal Habenula Limits Attraction to an Odor in Zebrafish

Krishnan, Seetha; Mathuru, Ajay S.; Kibat, Caroline; Rahman, Mashiur; Lupton, Charlotte; Stewart, Jim; Claridge‐Chang, Adam; Yen, Shih-Cheng; Jesuthasan, Suresh

doi:10.1016/j.cub.2014.03.073

Cited by 78 publications

(104 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The natural log fold change in fluorescence was fit to a normal distribution and the mean and standard deviation determined. Neurons were deemed responsive if the change in fluorescence intensity post-shock was greater than 1 standard deviation of the mean [52, 53], and responsive neurons on average had a greater than 2-fold increase in neural activity [54]. The initial time point at which neuronal activity increased, for a given neuron, was determined using the MATLAB function ‘ findpeaks ’.…”

Section: Star ★ Methodsmentioning

confidence: 99%

Left Habenular Activity Attenuates Fear Responses in Larval Zebrafish

et al. 2017

View full text Add to dashboard Cite

Summary Fear responses are defensive states that ensure survival of an organism in the presence of a threat. Perception of an aversive cue causes changes in behavior and physiology, such as freezing and elevated cortisol, followed by a return to the baseline state when the threat is evaded [1]. Neural systems that elicit fear behaviors include the amygdala, hippocampus and medial prefrontal cortex. However, aside from a few examples, little is known about brain regions that promote recovery from an aversive event [2]. Previous studies had implicated the dorsal habenular nuclei in regulating fear responses and boldness in zebrafish [3–7]. We now show that perturbation of the inherent left-right (L-R) asymmetry of the dorsal habenulo-interpeduncular (dHb-IPN) pathway at larval stages expedites the return of locomotor activity following an unexpected negative stimulus, electric shock. Severing habenular efferents to the IPN, or only those from the left dHb, prolongs the freezing behavior that follows shock. Individuals with symmetric, right isomerized dHb also exhibit increased freezing. In contrast, larvae that have symmetric, left-isomerized dHb, or in which just the left dHb-IPN projection is optogenetically activated, rapidly resume swimming post shock. In vivo calcium imaging reveals a neuronal subset, predominantly in the left dHb, whose activation is correlated with resumption of swimming. The results demonstrate functional specialization of the left dHb-IPN pathway in attenuating the response to fear.

show abstract

Section: Star ★ Methodsmentioning

confidence: 99%

Left Habenular Activity Attenuates Fear Responses in Larval Zebrafish

et al. 2017

View full text Add to dashboard Cite

show abstract

“…2(b')]. Clusters of activity could be found in the olfactory bulb (OB) and habenula (Hb), the latter considered to be involved in relaying odorevoked behavioral responses, 19,20 as well as in some neurons of the optic tectum (OT) [Figs. 2(b)-2(c)].…”

Section: Resultsmentioning

confidence: 99%

“…We stimulated the larvae with the odorant cadaverine (Cad, 10-μM solution), a diamine generated by decarboxylation of the amino acid lysine during decomposition of flesh, which is known to evoke olfactory-related neural activity and avoidance responses in zebrafish larvae. 19,20 Figure 2 shows three image planes through the volumetrically reconstructed light field dataset [ Fig. 2(a)], as well as a top-down maximum intensity projection [ Fig.…”

Section: Resultsmentioning

confidence: 99%

Calcium neuroimaging in behaving zebrafish larvae using a turn-key light field camera

et al. 2015

View full text Add to dashboard Cite

Abstract. Reconstructing a three-dimensional scene from multiple simultaneously acquired perspectives (the light field) is an elegant scanless imaging concept that can exceed the temporal resolution of currently available scanning-based imaging methods for capturing fast cellular processes. We tested the performance of commercially available light field cameras on a fluorescent microscopy setup for monitoring calcium activity in the brain of awake and behaving reporter zebrafish larvae. The plenoptic imaging system could volumetrically resolve diverse neuronal response profiles throughout the zebrafish brain upon stimulation with an aversive odorant. Behavioral responses of the reporter fish could be captured simultaneously together with depth-resolved neuronal activity. Overall, our assessment showed that with some optimizations for fluorescence microscopy applications, commercial light field cameras have the potential of becoming an attractive alternative to custom-built systems to accelerate molecular imaging research on cellular dynamics. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

show abstract

“…Cells that can be identified based on morphology, location, or functional characteristics, can be targeted for ablations using high-power, pulsed lasers. For instance, single neuron ablations in the reticulospinal system have been used to identify the reticulospinal neurons necessary for certain defined types of swim (Liu and Fetcho, 1999;Orger et al, 2008;Kohashi and Oda, 2008;Huang et al, 2013), or even particular kinematic parameters of individual swim bouts (Severi et al, 2014;Thiele et al, 2014), and similar methods have also been used to generate lesions in more broadly defined areas (Roeser and Baier, 2003;Mu et al, 2012;Krishnan et al, 2014). A particularly powerful approach is to combine genetic specificity with the spatial control of optical methods, for example, using the phototoxic protein KillerRed (Lee et al, 2010).…”

Section: Many Transgenic Lines Exist Which Allow Expression Of Genes mentioning

confidence: 98%

“…Krishnan et al developed a simple, wide-field epifluorescence system for rapid three-dimensional imaging using fast focusing and deconvolution, and applied this method to reveal, with single cell resolution, the dynamics in response to different concentrations of multiple odors throughout the whole habenula (Krishnan et al, 2014). They found that population activity in the right dorsal habenula varied with the concentration of a socially relevant odor (a bile salt), and provided evidence, using pharmacology and ablations, that this region mediates a switch from attraction to avoidance at high concentrations.…”

Section: Comprehensive Imaging From Neural Populationsmentioning

confidence: 99%