The risk of endometriosis and exposure to dioxins and polychlorinated biphenyls: a case–control study of infertile women

Pauwels, An; Schepens, Paul; D’Hooghe, Thomas; Delbeke, L.; Dhont, Marc; Brouwer, Abraham; Weyler, Joost

doi:10.1093/humrep/16.10.2050

Cited by 140 publications

(84 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BioDetection Systems uses a genetically engineered rat cell line without separation of dioxin-like PCBs for estimation of TEQ activity (CALUX s by BDS). The CALUX s by BDS bioassay has been used to estimate serum TEQ in epidemiologic studies of endometriosis (Pauwels et al, 2001), immunologic markers (Van Den Heuvel et al, 2002), and sexual maturation Nawrot et al, 2002). However, limited comparison studies of the CALUX s by BDS bioassay with the chemical analysis measurements have been completed (Pauwels et al, 2000;Koppen et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Dioxin-Like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX

Warner¹,

Eskenazi²,

Patterson

et al. 2004

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

Polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs), and polychlorinated biphenyls (PCBs) are widespread environmental contaminants that exist as complex mixtures and are frequently detected at part-per-trillion (ppt) levels in humans. Using isotope dilution high-resolution gas chromatography/ high-resolution mass spectrometry (HRGC/HRMS), we measure the PCDDs, PCDFs, and PCBs in serum of a population of 78 women residing in an area near Seveso, Italy where a TCDD explosion occurred in 1976 and where furniture is manufactured. The average total dioxin-like toxic equivalents (TEQ) of these women was 25.3 ppt, lipid-adjusted, comparable to other parts of Europe. TCDD levels, however, were higher among the few women who resided in the exposed area in 1976. We examined the possibility of using the CALUX (chemicalactivated luciferase gene expression) bioassay to estimate total TEQ in a small volume of plasma from this population. A total of 32 archived plasma specimens were selected for CALUX bioassay, based on the distribution of Total TEQ by HRGC/HRMS. The CALUX bioassay was performed blind to HRGC/HRMS results with 2 ml plasma per replicate analysis. Of 32 samples, 10 were below detection limits in the CALUX bioassay. For the 32 samples, the CALUX-TEQ averaged 25.4 ppt, lipid-adjusted (range: 0-127.6) and was not significantly different from the HRGC/HRMS Total TEQ average of 31.2 ppt, lipid-adjusted (range: 12.7-88.3) (t ¼ 0.88, P ¼ 0.38), however, the two measures were not significantly correlated (R s ¼ 0.04, P ¼ 0.82). More validation of the CALUX bioassay with larger sample volume is needed before application as an exposure measure in large-scale epidemiologic studies of health effects of dioxin-like compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Dioxin-Like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX

Warner¹,

Eskenazi²,

Patterson

et al. 2004

J Expo Sci Environ Epidemiol

View full text Add to dashboard Cite

show abstract

“…On the other hand, there was no evidence for impact of exposure to four major PCB congeners on the occurrence of endometriosis (ORs ranging from 1.007 to 1.026, none of them significant). Therefore, the reported association between exposure to dioxins and related compounds and endometriosis cannot be confounded by the exposure to major PCBs (18).…”

mentioning

confidence: 99%

Comparison of chemical-activated luciferase gene expression bioassay and gas chromatography for PCB determination in human serum and follicular fluid.

Pauwels

Cenijn

Schepens

et al. 2000

Environ Health Perspect

View full text Add to dashboard Cite

We assessed exposure to dioxin-like compounds using chemical and bioassay analysis in different matrices in a female population. A total of 106 serum and 9 follicular fluid samples were collected from infertile women attending Centers for Reproductive Medicine in Belgium from 1996 to 1998. Major polychlorinated biphenyl (PCB) congeners were quantified by chemical analysis using gas chromatography with electron-capture detection, and the chemical-activated luciferase gene expression (CALUX) bioassay was used to determine the total dioxin-like toxic equivalence (TEQ) of mixtures of polyhalogenated aromatic hydrocarbons present in body fluids, such as serum and follicular fluid. To the best of our knowledge, this is the first investigation to determine TEQ values by the CALUX bioassay in follicular fluid. The TEQ levels in both matrices are well correlated (r = 0.83, p = 0.02). As the chemical and bioassay analysis executed in this study do not cover the same span of polyhalogenated aromatic hydrocarbons, we did not expect totally correlated results. Moreover, the sample workup and quantification of the analytes differed completely. Nonetheless, the TEQ values in human extracts correlated well with the sum of four major PCB congeners chemically determined in both serum and follicular fluid. These results indicate that the CALUX bioassay may serve as a simple, relatively inexpensive prescreening tool for exposure assessment in epidemiologic surveys. Images Figure 1 Figure 2

show abstract

“…The authors evaluated that the lack of association in other studies (Pauwels et al 2001, Eskenazi et al 2002b, Tsukino et al 2005, cf. Annex 8B) may have been due e.g.…”

Section:  Effects Assessment 4 423 Reproductive Effectsmentioning

confidence: 99%

Risks and Management of Dioxins and Dioxin-like Compounds in Baltic Sea Fish: An Integrated Assessment

2006

TemaNord

View full text Add to dashboard Cite

The risk of endometriosis and exposure to dioxins and polychlorinated biphenyls: a case–control study of infertile women

Abstract: The study results showed no statistically significant association between exposure to dioxin-like compounds and the occurrence of endometriosis in infertile women.

Cited by 140 publications

References 25 publications

Dioxin-Like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX

Dioxin-Like TEQ of women from the Seveso, Italy area by ID-HRGC/HRMS and CALUX

Comparison of chemical-activated luciferase gene expression bioassay and gas chromatography for PCB determination in human serum and follicular fluid.

Risks and Management of Dioxins and Dioxin-like Compounds in Baltic Sea Fish: An Integrated Assessment

Contact Info

Product

Resources

About