1988
DOI: 10.1016/s0021-9258(18)68367-x
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The RNA N-glycosidase activity of ricin A-chain. The characteristics of the enzymatic activity of ricin A-chain with ribosomes and with rRNA.

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Cited by 373 publications
(83 citation statements)
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“…However, we have proposed a model for the interaction with the single-stranded RNA of the ribosome which can be tested. Further light may be shed on the problem of recognition of ribosomal RNA by the observation that all of the type I RIPs tested can depurinate 23S rRNA in E coli ribosomes, while E. coli ribosomes are completely insensitive to the A chains of ricin (RTA) and abrin [10,11] (although when naked 23S rRNA is treated with a high concentration of RTA, the target adenine is released [50]). This implies that there are structural differences between type I and type II RIPs that determine their specificity for ribosomes.…”
Section: Biological Implicationsmentioning
confidence: 99%
“…However, we have proposed a model for the interaction with the single-stranded RNA of the ribosome which can be tested. Further light may be shed on the problem of recognition of ribosomal RNA by the observation that all of the type I RIPs tested can depurinate 23S rRNA in E coli ribosomes, while E. coli ribosomes are completely insensitive to the A chains of ricin (RTA) and abrin [10,11] (although when naked 23S rRNA is treated with a high concentration of RTA, the target adenine is released [50]). This implies that there are structural differences between type I and type II RIPs that determine their specificity for ribosomes.…”
Section: Biological Implicationsmentioning
confidence: 99%
“…Pokeweed antiviral protein (PAP) is a 29 kDa ribosomeinactivating protein (RIP) isolated from the leaves of the pokeweed plant (Phytolacca americana). PAP and other RIPs such as ricin, Shiga toxin from Shigella disentaria and Shiga-like toxin from Escherichia coli catalytically remove an adenine (A4324) residue from the highly conserved sarcin/ ricin loop (S/R) of the large rRNA (1)(2)(3)(4)(5). PAP can also remove an additional adenine and a guanine from the S/R loop (6).…”
Section: Introductionmentioning
confidence: 99%
“…This inhibition is a consequence of ribosome injury due to the enzymatic activity of RIPs, which makes ribosomes unable to interact with transcription factors such as the elongation factor 2 (EF-2, in eukaryotes) or the elongation factor G (EF-G, in prokaryotes), thus blocking the translocation processes [2]. In particular, the enzymatic action of RIPs results in the cleavage of a specific adenine residue (A 4324 in rat) in a conserved site of the 28S rRNA, known as the Sarcin Ricin Loop (SRL), for which they are classified as rRNA N-glycosylase (EC: 3.2.2.22) [3]. The specific enzymatic action of RIPs promotes cell death by apoptosis through the activation of caspases and caspase-like activities as well as the poly(ADP-ribose) polymerase cleavage [4].…”
Section: Introductionmentioning
confidence: 99%