The role and mechanism of abscisic acid in mitigating the adverse impacts of high temperature in Gracilariopsis lemaneiformis

Sun, Peng; Chen, Qionglin; Luo, Suya; Yu, Xinlei; Zhang, Xiaoqian; Xu, Nianjun; Sun, Xue

doi:10.1007/s10811-021-02606-w

Cited by 6 publications

(2 citation statements)

References 51 publications

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“…Abscisic acid is also a critical signal molecule in abiotic stress responses, affecting gene expression as well as physiological and biochemical reactions [12,13]. Plants produce more ABA when they are stressed, such as by heat [14], cold [15], or drought [16]. On the other hand, the ABA-deficient mutants of Arabidopsis were less freezing resistant than their wild types [17].…”

Section: Introductionmentioning

confidence: 99%

An in Vitro Approach to Investigate the Role of Abscisic Acid in Alleviating the Negative Effects of Chilling Stress on Banana Shoots

Hmmam¹,

Raza²,

Djalović³

et al. 2023

Phyton

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Banana is a tropical crop cultivated in warm places. Chilling stress in Egypt is making banana crops less productive. Abscisic acid (ABA), a key plant hormone, regulates metabolic and physiological processes and protects plants from a variety of stresses. In vitro growing banana shoots were pre-treated with ABA at four concentrations (0, 25, 50, and 100 mM) and chilled at 5°C for 24 h, followed by a six-day recovery period at 25°C. By comparing ABA treatments to both positive and negative controls, physiological and biochemical changes were investigated. Chilling stress (5°C) caused a considerable increase in lipid peroxidation and ion leakage and reduced photosynthetic pigments in cold-treated plantlets. Increasing the concentration of ABA to 100 µM enhanced the response to chilling stress. ABA had a major effect on mitigating chilling injury in banana shoots by keeping cell membranes stable and lowering the amount of ion leakage and lipid peroxidation. Also, ABA significantly maintained the photosynthetic pigment concentration of banana shoots; accumulated higher amounts of total soluble carbohydrates and proline; and increased DPPH radical scavenging activity. Furthermore, ABA treatment enhanced cold tolerance in chilling-stressed banana shoots through the regulation of antioxidant enzyme activity. Overall, the results show that ABA is a good choice for protecting banana shoots from the damage caused by chilling stress.

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Section: Introductionmentioning

confidence: 99%

An in Vitro Approach to Investigate the Role of Abscisic Acid in Alleviating the Negative Effects of Chilling Stress on Banana Shoots

Hmmam¹,

Raza²,

Djalović³

et al. 2023

Phyton

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“…Phytohormones were reported to be involved in the defense against abiotic stress. Abscisic acid (ABA) can be induced by heat stress in plants [ 23 ] and can reduce the damage caused by heat stress in G. lemaneiformis [ 24 ]. The content of 1-aminocyclopropane-1-carboxylic acid (ACC), the direct precursor of ethylene, increases under heavy metal stress conditions [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

A survey of the full-length transcriptome of Gracilariopsis lemaneiformis using single-molecule long-read sequencing

et al. 2022

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Background Posttranscriptional processing of precursor mRNAs contributes to transcriptome and protein diversity and gene regulatory mechanisms in eukaryotes. However, this posttranscriptional mechanism has not been studied in the marine macroalgae Gracilariopsis lemaneiformis, which is the most cultivated red seaweed species in China. Results In the present study, third-generation sequencing (Pacific Biosciences single-molecule real-time long-read sequencing, SMRT-Seq) was used to sequence the full-length transcriptome of G. lemaneiformis to identify alternatively spliced transcripts and alternative polyadenylation (APA) sites in this species. RNAs were isolated from G. lemaneiformis under various treatments including abiotic stresses and exogenous phytohormones, and then equally pooled for SMRT-Seq. In summary, 346,544 full-length nonchimeric reads were generated, from which 13,630 unique full-length transcripts were obtained in G. lemaneiformis. Compared with the known splicing events in the gene models, more than 3000 new alternative splicing (AS) events were identified in the SMRT-Seq reads. Additionally, 810 genes were found to have poly (A) sites and 91 microRNAs (miRNAs), 961 long noncoding RNAs and 1721 novel genes were identified in G. lemaneiformis. Moreover, validation experiments showed that abiotic stresses and phytohormones could induce some specific AS events, especially intron retain isoforms, cause some alterations to the relative ratios of transcripts annotated to the same gene, and generate novel 3′ ends because of differential APA. The growth of G. lemaneiformis was inhibited by Cu stress, while this inhibition was alleviated by ACC treatment. RNA-Seq analysis further revealed that 211 differential alternative splicing (DAS) events and 142 DAS events was obtained in CK vs Cu and Cu vs Cu + ACC, respectively, suggesting that AS of functional genes could be regulated by Cu stress and ACC. Compared with Cu stress, the expression of transcripts with DAS events mainly involved in the carbon fixation in photosynthetic organisms and oxidative phosphorylation pathway was upregulated in Cu + ACC treatment, revealing that ACC alleviated the growth inhibition by Cu stress by increasing carbon fixation and oxidative phosphorylation. Conclusions Our results provide the first comprehensive picture of the full-length transcriptome and posttranscriptional mechanism in red macroalgae, including transcripts that appeared in the presence of common abiotic stresses and phytohormones, which will improve the gene annotations of Gracilariopsis and contribute to the study of gene regulation in this important cultivated seaweed.

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The Beneficial Effect of 24-Epibrassinolide Against High-Temperature Stress in Gracilariopsis lemaneiformis Revealed by Physiological Response and Transcriptomic Profiling

Chu,

Luo,

Chen

et al. 2024

J Plant Growth Regul

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The role and mechanism of abscisic acid in mitigating the adverse impacts of high temperature in Gracilariopsis lemaneiformis

Cited by 6 publications

References 51 publications

An in Vitro Approach to Investigate the Role of Abscisic Acid in Alleviating the Negative Effects of Chilling Stress on Banana Shoots

An in Vitro Approach to Investigate the Role of Abscisic Acid in Alleviating the Negative Effects of Chilling Stress on Banana Shoots

A survey of the full-length transcriptome of Gracilariopsis lemaneiformis using single-molecule long-read sequencing

The Beneficial Effect of 24-Epibrassinolide Against High-Temperature Stress in Gracilariopsis lemaneiformis Revealed by Physiological Response and Transcriptomic Profiling

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