The Role and Mechanism of miRNA-1224 in the <i>Polygonatum sibiricum</i> Polysaccharide Regulation of Bone Marrow-Derived Macrophages to Osteoclast Differentiation

Li, Bo; Wu, Pingping; Fu, Wen-Wen; Xiong, Yin; Zhang, Lei; Gao, Yunbing; Deng, Guo-Xiong; Zong, Shaohui; Zeng, Gaofeng

doi:10.1089/rej.2018.2126

Cited by 37 publications

(20 citation statements)

References 36 publications

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“…At present, it has been found that miRNA-1224-5p is abnormally expressed in a variety of tumors, and it can be used as an oncogene or as an antioncogene and can be regulated at the protein expression levels of Caspase-3, Bcl-2, and Bax [ 26 ]. It was found that the expression of miRNA-1224-5p in lung cancer changed significantly and inhibited the formation of keloid fibroblasts [ 27 , 28 ].…”

Section: Discussionmentioning

confidence: 99%

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

Zhang

Wang

et al. 2020

BioMed Research International

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Objective. To study the biological function of circular RNA RNF13 (circRNF13) in acute myeloid leukemia (AML) and its relationship with prognosis. Methods. We constructed stable AML cell lines with downregulated expression of circRNF13, and then, we explored the effect of downregulation of circRNF13 expression on the proliferation, migration, and invasion through qRT-PCR, MTT curve, colony formation, transwell migration and invasion experiment, cell cycle, apoptosis, Caspase 3/7 assay, and other experiments. We also studied the expression of C-myc and Tenascin-C by qRT-PCR to explore the role of circRNF13. Results. When the expression of circRNF13 was downregulated, the proliferation rate of AML cells decreased significantly, the cell cycle was blocked to G1 phase, and apoptosis rate increased significantly. C-myc related to cell proliferation decreased significantly at RNA level. Furthermore, when the expression of circRNF13 was downregulated, the migration and invasion ability of AML cells was significantly reduced, and the expression of Tenascin-C related to migration and invasion also decreased significantly. The luciferase reporter assay system confirmed that miRNA-1224-5p was the direct target of circRNF13. Conclusion. CircRNF13 inhibited the proliferation, migration, and invasion of AML cells by regulating the expression of miRNA-1224-5p. This study provides some clues for the diagnosis and treatment of AML.

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Section: Discussionmentioning

confidence: 99%

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

Zhang

Wang

et al. 2020

BioMed Research International

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“…Moreover, Limd1 is considered as an important negative regulator of Hippo pathway (Thakur et al, 2010;Jagannathan et al, 2016). It has been concluded recently in vitro that Polygonatum sibiricum polysaccharide (PSP) suppresses Hippo-YAP pathway to inhibit differentiation of osteoclasts from BMMs through downregulating expression of miRNA-1224, of which the target gene is Limd1 (Li B. et al, 2019). Additionally, the further mechanism of Hippo-YAP signaling regulating osteoclastogenesis is probably implicated to the transcriptional regulation by TEAD1 that binds to an upstream enhancer element of osteoprotegerin (OPG) and promotes its expression, which was revealed in human periodontal ligament cells (PDLCs) .…”

Section: The Influence Of Hippo-yap Signaling On Osteogenesismentioning

confidence: 99%

Role of Hippo-YAP Signaling in Osseointegration by Regulating Osteogenesis, Angiogenesis, and Osteoimmunology

Zhou

Liu

et al. 2020

Front. Cell Dev. Biol.

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The social demand for dental implantation is growing at a rapid rate, while dentists are faced with the dilemma of implantation failures associated with unfavorable osseointegration. Clinical-friendly osteogenesis, angiogenesis and osteoimmunology around dental implants play a pivotal role in a desirable osseointegration and it's increasingly appreciated that Hippo-YAP signaling pathway is implicated in those biological processes both in vitro and in vivo in a variety of study. In this article we review the multiple effects of Hippo-YAP signaling in osseointegration of dental implants by regulating osteogenesis, angiogenesis and osteoimmunology in peri-implant tissue, as well as highlight prospective future directions of relevant investigation.

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“…Interestingly, both osteoclastogenesis and tumorigenesis of CRC are remarkedly regulated by miR-NAs. Previous studies identified miR- 483-5p, miR-124, miR-218, miR-199a-5p, miR-133a can enhance the osteoclastogenesis while miR-340 inhibited the osteoclast formation via inhibiting MITF (Li et al 2018(Li et al , 2019(Li et al , 2020Guo et al 2018Guo et al , 2019Zhao et al 2017). In addition, miR-16 and miR-378 promoted osteoclastogenesis in bone metastasis of breast cancer (Ell et al 2013).…”

Section: Introductionmentioning

confidence: 97%

miR-325-3p, a novel regulator of osteoclastogenesis in osteolysis of colorectal cancer through targeting S100A4

Chengling

Zhang

Xie

et al. 2021

Mol Med

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Background To investigate effect of microRNA-325-3p (miR-325-3p) on bone metastasis of colorectal cancer (CRC) and the precise role on osteoclastogenesis. Methods CT-26 cells were injected into tibias to establish bone metastatic model of CRC in vivo. AgomiR-325-3p or antagomir-325-3p were injected in tail-veins of Balb/c mice to interfere the osteoclastogenesis and bone metastasis of CRC. Safranin O and Fast Green staining examined the changes of trabecular area and TRAP staining examined the osteoclast number in bone metastasis of CRC. Real-time PCR was conducted to test the RNA level of miR-325-3p and mRNA levels of TRAP and Cathepsin K in osteoclast precursors (OCPs). Dual-luciferase reporter system was utilized to identify the direct target of miR-325-3p. Conditioned medium from CT-26 cells was collected to stimulate the OCPs during osteoclastogenesis induced by RANKL and M-CSF in vitro. Western blot analysis was performed to examine the protein level of S100A4 in OCPs after interfered by agomiR-325-3p or antagomir-325-3p cultured in CM or not. Results miR-325-3p downregulated in OCPs in CRC microenvironment both in vivo and in vitro. By luciferase activity assay, S100A4 was the target gene of miR-325-3p and the protein level of S100A4 in OCPs upregulated in CRC microenvironment. Overexpression of miR-325-3p inhibited the osteoclastogenesis of OCPs and it can be reversed after transfection with plasmid containing S100A4. Treatment with miR-325-3p can preserve trabecular area in bone metastasis of CRC. Conclusion miR-325-3p can prevent osteoclast formation through targeting S100A4 in OCPs. Overexpression of miR-325-3p efficiently decreased the osteoclast number and attenuated bone resorption in bone metastasis of CRC.

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The Role and Mechanism of miRNA-1224 in the Polygonatum sibiricum Polysaccharide Regulation of Bone Marrow-Derived Macrophages to Osteoclast Differentiation

Cited by 37 publications

References 36 publications

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

The Regulation of circRNA RNF13/miRNA-1224-5p Axis Promotes the Malignant Evolution in Acute Myeloid Leukemia

Role of Hippo-YAP Signaling in Osseointegration by Regulating Osteogenesis, Angiogenesis, and Osteoimmunology

miR-325-3p, a novel regulator of osteoclastogenesis in osteolysis of colorectal cancer through targeting S100A4

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