The role for CYCLIN A1;2/TARDY ASYNCHRONOUS MEIOSIS in differentiated cells in Arabidopsis

Jha, Ajay Kumar; Wang, Yixing; Hercyk, Brian S.; Shin, Hwa-Soo; Chen, Rujin; Yang, Ming

doi:10.1007/s11103-013-0170-9

Cited by 11 publications

(17 citation statements)

References 54 publications

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“…Homologous recombination was essential for accurate repair of DNA double stranded breaks (DSBs) created by highly conserved SPO11 proteins during meiosis in most eukaryotes (Shingu et al, 2012), and known genes DMC1, RAD54 , and RPA , as key mediators to regulate this process were significantly down-regulated in this pathway. The Cyclin-A1-2/TAM , known to regulate the progression of cell cycle during meiosis was the only cyclin-related gene within DNA replication pathway that was up-regulated (Jha et al, 2014). Besides, a portion of genes enriched were characterized as critical players in regulating transcription, DNA replication, DNA repair, and chromosome stability (Table S7).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa

et al. 2017

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Polyploidy is a fundamental process in plant evolution. Understanding the polyploidy-associated effects on plant reproduction is essential for polyploid breeding program. In the present study, our cytological analysis firstly demonstrated that an overall course of meiosis was apparently distorted in the synthetic polyploid Brassica rapa in comparison with its diploid progenitor. To elucidate genetic basis of this irregular meiosis at a molecular level, the comparative RNA-seq analysis was further used to investigate differential genetic regulation of developing floral buds identified at meiosis between autotetraploid and diploid B. rapa. In total, compared to its diploid counterparts, among all 40,927 expressed genes revealed, 4,601 differentially expressed genes (DEGs) were identified in the floral buds of autotetraploid B. rapa, among which 288 DEGs annotated were involved in meiosis. Notably, DMC1 identified as one previously known meiosis-specific gene involved in inter-homologous chromosome dependent repair of DNA double stranded breaks (DSBs), was significantly down-regulated in autotetraploid B. rapa, which presumably contributed to abnormal progression during meiosis I. Although certain DEGs associated with RNA helicase, cell cycling, and somatic DNA repair were up-regulated after genome duplication, genes associated with meiotic DSB repair were significantly down-regulated. Furthermore, the expression of randomly selected DEGs by RNA-seq analysis was confirmed by quantitative real-time PCR analysis in both B. rapa and Arabidopsis thaliana. Our results firstly account for adverse effects of polyploidy on an entire course of meiosis at both cytological and transcriptomic levels, and allow for a comprehensive understanding of the uniformity and differences in the transcriptome of floral buds at meiosis between diploid and polyploid B. rapa as well.

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Section: Resultsmentioning

confidence: 99%

Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa

et al. 2017

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“…TAM, BAM1, and BAM2 expression is altered in abcb1 abcb19 young flower buds It has been shown that the TAM cyclin synchronizes cell divisions during male meiosis (Jha et al 2014). To assess whether the asynchronous progression of meiosis observed in abcb1 and abcb1 abcb19 anthers is due to the altered expression of TAM, we analyzed the abundance of its transcript in abcb1 abcb19 young flower buds.…”

Section: Resultsmentioning

confidence: 99%

ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development

Cecchetti

Brunetti

Napoli

et al. 2015

JIPB

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Arabidopsis abcb1 abcb19 double mutants defective in the auxin transporters ABCB1/PGP1 and ABCB19/PGP19 are altered in stamen elongation, anther dehiscence and pollen maturation. To assess the contribution of these transporters to stamen development we performed phenotypic, histological analyses, and in situ hybridizations on abcb1 and abcb19 single mutant flowers. We found that pollen maturation and anther dehiscence are precocious in the abcb1 but not in the abcb19 mutant. Accordingly, endothecium lignification is altered only in abcb1 anthers. Both abcb1 and abcb1 abcb19 stamens also show altered early development, with asynchronous anther locules and a multilayer tapetum. DAPI staining showed that the timing of meiosis is asynchronous in abcb1 abcb19 anther locules, while only a small percentage of pollen grains are nonviable according to Alexander's staining. In agreement, TAM (TARDY ASYNCHRONOUS MEIOSIS), as well as BAM2 (BARELY ANY MERISTEM)-involved in tapetal cell development-are overexpressed in abcb1 abcb19 young flower buds. Correspondingly, ABCB1 and ABCB19 mRNA localization supports the observed phenotypes of abcb1 and abcb1 abcb19 mutant anthers. In conclusion, we provide evidence that auxin transport plays a significant role both in early and late stamen development: ABCB1 plays a major role during anther development, while ABCB19 has a synergistic role.Keywords: Anther development; Arabidopsis; auxin transport; endothecium lignification; tapetum differentiation Citation: Cecchetti V, Brunetti P, Napoli N, Fattorini L, Altamura MM, Costantino P, Cardarelli M (2015) ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development.

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“…The above findings collectively show that cyclin As are a major class of cyclins that likely play indispensable roles in meiosis. In the current report, we show that the overexpression of the Arabidopsis A-type cyclin TAM by the ASK1 promoter [19] had a dominant effect that led to the production of the same male meiotic products as in the tam loss-of-function mutants. However, upon examination of the chromosome morphology and the subcellular localization of the ASY1 protein in male meiocytes from zygotene to diplotene, different effects of the overexpression and the loss of function of TAM were revealed.…”

Section: Introductionmentioning

confidence: 54%

“…It was previously reported that overexpression of TAM by the ASK1 promoter led to the production of polyploid plants [19] . To investigate the cellular basis for such an effect, a total of 36 T 1 plants were obtained from six independent transformation experiments that introduced the ASK1:TAM transgene into the wild-type plants.…”

Section: Resultsmentioning

confidence: 99%

“…Four T 1 plants from one remaining transformation experiment produced only tetrads while two plants from still another transformation experiment produced only dyads or a mixture of dyads, triads, and tetrads. The abnormal meiotic products should also have occurred in female meiosis in some of these lines because progeny from at least one of the lines, ASK1:TAM1 , were tetraploid [19] . Furthermore, 34 of these plants produced variable amounts of dark and collapsed seeds ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Loss-of-Function Mutants and Overexpression Lines of the Arabidopsis Cyclin CYCA1;2/TARDY ASYNCHRONOUS MEIOSIS Exhibit Different Defects in Prophase-I Meiocytes but Produce the Same Meiotic Products

Wang

Yang

2014

PLoS ONE

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In Arabidopsis, loss-of-function mutations in the A-type cyclin CYCA1;2/TARDY ASYNCHRONOUS MEIOSIS (TAM) gene lead to the production of abnormal meiotic products including triads and dyads. Here we report that overexpression of TAM by the ASK1:TAM transgene also led to the production of triads and dyads in meiosis, as well as shriveled seeds, in a dominant fashion. However, the partial loss-of-function mutant tam-1, an ASK1:TAM line, and the wild type differed in dynamic changes in chromosome thread thickness from zygotene to diplotene. We also found that the pericentromeric heterochromatin regions in male meiocytes in tam-1 and tam-2 (a null allele) frequently formed a tight cluster at the pachytene and diplotene stages, in contrast to the infrequent occurrences of such clusters in the wild type and the ASK1:TAM line. Immunolocalization studies of the chromosome axial component ASY1 revealed that ASY1 was highly expressed at the appropriate male meiotic stages but not localized to the chromosomes in tam-2. The level of ASY1, however, was greatly reduced in another ASK1:TAM line with much overexpressed TAM. Our results indicate that the reduction and increase in the activity of TAM differentially affect chromosomal morphology and the action of ASY1 in prophase I. Based on these results, we propose that either the different meiotic defects or a common defect such as missing ASY1 on the chromosomal axes triggers a hitherto uncharacterized cell cycle checkpoint in the male meiocytes in the tam mutants and ASK1:TAM lines, leading to the production of the same abnormal meiotic products.

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The role for CYCLIN A1;2/TARDY ASYNCHRONOUS MEIOSIS in differentiated cells in Arabidopsis

Cited by 11 publications

References 54 publications

Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa

Transcriptome Analysis of Floral Buds Deciphered an Irregular Course of Meiosis in Polyploid Brassica rapa

ABCB1 and ABCB19 auxin transporters have synergistic effects on early and late Arabidopsis anther development

Loss-of-Function Mutants and Overexpression Lines of the Arabidopsis Cyclin CYCA1;2/TARDY ASYNCHRONOUS MEIOSIS Exhibit Different Defects in Prophase-I Meiocytes but Produce the Same Meiotic Products

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