The role of 5‐HT_2c receptor on corticosterone‐mediated food intake

Abstract

Corticosterone plays an important role in feeding behavior. However, its mechanism remains unclear. Therefore, the present study aimed to investigate the effect of corticosterone on feeding behavior. In this study, cumulative food intake was increased by acute corticosterone administration in a dose-dependent manner. Administration of the 5-HT receptor agonist m-chlorophenylpiperazin (mCPP) reversed the effect of corticosterone on food intake. The anorectic effects of mCPP were also blocked by the 5-HT receptor antagonist RS102221 in corticosterone-treated mice. Both corticosterone and mCPP increased c-Fos expression in hypothalamic nuclei, but not the nucleus of the solitary tract. RS102221 inhibited c-Fos expression induced by mCPP, but not corticosterone. In addition, mCPP had little effect on TH and POMC levels in the hypothalamus. Furthermore, mCPP antagonized decreasing effect of the leptin produced by corticosterone. Taken together, our findings suggest that 5-HT receptors and leptin may be involved in the effects of corticosterone-induced hyperphagia.

Quantitative profiling of neurotransmitter abnormalities in brain, cerebrospinal fluid, and serum of experimental diabetic encephalopathy male rat

“…In the CNS, 5-HT is produced in the raphe nuclei of the brainstem and hypothalamus. By functioning as a neurotransmitter in CNS, 5-HT regulates mood (Naoi, Maruyama, & Shamoto-Nagai, 2017), sleep-wake behavior (Bazan & Fares, 2016), and food intake (Ge et al, 2017). In the periphery, 5-HT is mainly produced in the gut and pineal gland.…”

Role of leptin in the regulation of food intake in fasted mice

“…After all drug treatment, each animal was placed in a new cage and refed with 4‐6 food pellets. The food intake was measured at 0.5, 1 and 1.5 hours after the final injection as we described previously . For calculation of the cumulative food intake, the latter weight of feed was subtracted from the former value.…”

“…The lysates were then further centrifuged and boiled. Western blot method was same as our previous studies . The samples were resolved by 10% sodium dodecyl sulfate—polyacrylamide gel electrophoresis (SDS‐PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes, which were further blocked with 5% skim milk.…”

“…c‐Fos immunohistochemistry analysis method was presented as our previous studies . All mice were intraperitoneal injected with 400 mg/kg chloral hydrate for anesthetization.…”

Targeting the 5-HT2C Receptor in Biological Context and the Current State of 5-HT2C Receptor Ligand Development

“…Weight gain as well as a greater relative risk of metabolic dysfunction and diabetes occurs upon chronic treatment with atypical antipsychotics with 5-HT 2C R antagonist properties ( e.g ., olanzapine) in humans and animals [56–59]. Selective 5-HT 2C R agonists have been consistently demonstrated to suppress food intake [56, 60–62], in fact, the selective 5-HT 2C R agonist WAY163909 dose-dependently decreased food intake in normal Sprague–Dawley rats, obese Zuker rats and mice with diet-induced obesity [63]. Investigations of 5-HT involvement in the mechanisms underlying satiety have focused predominantly on neural loci in the hypothalamus and midbrain/hindbrain circuits which synchronize energy balance and glucose homeostasis in concert with peripheral systems (for reviews) [64, 65].…”