The role of ABA in the freezing injury avoidance in two Hypericum species differing in frost tolerance and potential to synthesize hypericins

Bruňáková, Katarína; Petijová, Linda; Zámečnı́k, Jiřı́; Turečková, Veronika; Čellárová, Eva

doi:10.1007/s11240-015-0748-9

Cited by 20 publications

(28 citation statements)

References 53 publications

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“…In the study of the cold response of Hypericum, it was found that the supplementation of exogenous ABA to preculture medium lowered the content of water in plant tissue. However, low concentration of ABA was not sufficient to induce the low-temperature tolerance (Bruňáková et al 2011(Bruňáková et al , 2015.…”

Section: Discussionmentioning

confidence: 93%

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Kamińska

Skrzypek

Wilmowicz

et al. 2016

Plant Cell Tiss Organ Cult

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Taraxacum pieninicum is an extremely threatened species. It seems that traditional protection methods of this species are insufficient and it is necessary to use biotechnology tools that allow the in vitro storage of plant material. The present study describes in vitro conservation of T. pieninicum by slow-growth storage. Various light conditions and abscisic acid (ABA) treatments and combinations thereof were tested. Viability, proliferation rate and ability of shoots to root were evaluated during regrowth. Moreover, the effect of shoot storage conditions on the ABA level was analysed. The results showed that light during 3-months' storage increased the level of endogenous ABA. Similar results were observed when storage at a low temperature was prolonged to 9 months. Changes in ABA level had a negative effect on shoot condition. However, these changes were related to leaves and did not affect the ability of shoot tips to proliferate after long-term storage. Addition of ABA to storage medium increased several folds the level of ABA in plant tissues, which resulted in a reduction in the visual rating and proliferation rate. Shoots obtained after post-storage regrowth were able to root. All rooted shoots survived adaptation to field conditions and were able to flower in the second year after acclimatization. The analysis of DNA content indicated that all the regenerants had the same ploidy level independent of treatments during storage. Cold storage of T. pieninicum as used in this study enabled the interval between subcultures to be extended to 9 months.

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Section: Discussionmentioning

confidence: 93%

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Kamińska

Skrzypek

Wilmowicz

et al. 2016

Plant Cell Tiss Organ Cult

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“…The phytohormone ABA is known to induce freezing resistance in many winter annual and perennial species ( Bravo et al, 1998 ) and was shown to contribute to acquisition of the tolerance to cryopreservation, e.g., in Triticum aestivum L. ( Chen et al, 1985 ), Bromus inermis Leyss., Medicago sativa L. ( Reaney and Gusta, 1987 ), Daucus carota L. ( Thierry et al, 1999 ), etc. Despite the exogenously applied ABA did not improve the resistance of H. perforatum shoot tips against freezing, the 3.5-fold higher level of endogenous ABA was observed in the freezing-tolerant H. perforatum , when compared with freezing-sensitive H. canariense ( Bruňáková et al, 2015 ).…”

Section: Crucial Processes For Successful Cryopreservation In mentioning

confidence: 82%

“…Among the plant growth regulators, ABA is involved in mediation of many physiological processes including adaptation responses to environmental conditions comprising dehydration, osmotic, and cold stresses ( Chandler and Robertson, 1994 ). The increasing level of endogenous ABA was observed under dehydration stress and cold treatment performed by the exposure of in vitro grown H. perforatum plants to subfreezing temperature of -4°C ( Bruňáková et al, 2015 ). The phytohormone ABA is known to induce freezing resistance in many winter annual and perennial species ( Bravo et al, 1998 ) and was shown to contribute to acquisition of the tolerance to cryopreservation, e.g., in Triticum aestivum L. ( Chen et al, 1985 ), Bromus inermis Leyss., Medicago sativa L. ( Reaney and Gusta, 1987 ), Daucus carota L. ( Thierry et al, 1999 ), etc.…”

Section: Crucial Processes For Successful Cryopreservation In mentioning

confidence: 99%

“…It has been observed that the exposure of freezing-tolerant H. perforatum or H. rumeliacum to a temperature of 4°C could entirely substitute the effect of ABA treatment during the pre-cryogenic phase ( Bruňáková et al, 2014 ). These conditions resulted in almost 45% recovery in H. perforatum and led to 1.3- and 1.5-fold increase in the content of ABA in H. perforatum and H. canariense ( Bruňáková et al, 2015 ). As a part of a cold-acclimation response, the elevated level of endogenous ABA is in agreement with a slight and transient increase of the hormone level in the model Arabidopsis thaliana ( Lang et al, 1994 ).…”

Section: Crucial Processes For Successful Cryopreservation In mentioning

confidence: 99%

“…The capacity to increase the tolerance to low temperatures depends on several factors, e.g., the temperature and regime of acclimation as well as the quantity and intensity of light ( Levitt, 1980 ; Monroy et al, 1993 ). In the model H. perforatum , a greater enhancement of the FT was observed upon exposure to gradually decreasing temperature from 22°C up to 4°C at a rate of 1°C day -1 ( Bruňáková et al, 2015 ). Based on the three-fold depress of the LT 50 value, H. perforatum was shown to possess the protective mechanisms associated with two major components: the constitutive FT expressed by LT 50 = -5.6°C, and cold-acclimation capacity to increase FT reaching LT 50 = -16.2°C.…”

Section: Post-cryogenic Recovery In Relation To Freezing Tolerance Inmentioning

confidence: 99%

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Conservation Strategies in the Genus Hypericum via Cryogenic Treatment

Bruňáková

Čellárová

2016

Front. Plant Sci.

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In the genus Hypericum, cryoconservation offers a strategy for maintenance of remarkable biodiversity, emerging from large inter- and intra-specific variability in morphological and phytochemical characteristics. Long-term cryostorage thus represents a proper tool for preservation of genetic resources of endangered and threatened Hypericum species or new somaclonal variants with unique properties. Many representatives of the genus are known as producers of pharmacologically important polyketides, namely naphthodianthrones and phloroglucinols. As a part of numerous in vitro collections, the nearly cosmopolitan Hypericum perforatum – Saint John’s wort – has become a suitable model system for application of biotechnological approaches providing an attractive alternative to the traditional methods for secondary metabolite production. The necessary requirements for efficient cryopreservation include a high survival rate along with an unchanged biochemical profile of plants regenerated from cryopreserved cells. Understanding of the processes which are critical for recovery of H. perforatum cells after the cryogenic treatment enables establishment of cryopreservation protocols applicable to a broad number of Hypericum species. Among them, several endemic taxa attract a particular attention due to their unique characteristics or yet unrevealed spectrum of bioactive compounds. In this review, recent advances in the conventional two-step and vitrification-based cryopreservation techniques are presented in relation to the recovery rate and biosynthetic capacity of Hypericum spp. The pre-cryogenic treatments which were identified to be crucial for successful post-cryogenic recovery are discussed. Being a part of genetic predisposition, the freezing tolerance as a necessary precondition for successful post-cryogenic recovery is pointed out. Additionally, a beneficial influence of cold stress on modulating naphthodianthrone biosynthesis is outlined.

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What determines root‐sprouting ability: Injury or phytohormones?

Martínková

Motyka

Bitomský

et al. 2022

American J of Botany

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Premise Root‐sprouting (RS) is an evolutionarily independent alternative to axillary stem branching for a plant to attain its architecture. Root‐sprouting plants are better adapted to disturbance than non‐RS plants, and their vigor is frequently boosted by biomass removal. Nevertheless, RS plants are rarer than plants that are not root‐sprouters, possibly because they must overcome developmental barriers such as intrinsic phytohormonal balance or because RS ability is conditioned by injury to the plant body. The objective of this study was to identify whether phytohormones or injury enable RS. Methods In a greenhouse experiment, growth variables, root respiration, and phytohormones were analyzed in two closely related clonal herbs that differ in RS ability (spontaneously RS Inula britannica and rhizomatous non‐RS I. salicina) with and without severe biomass removal. Results As previously reported, I. britannica is a root‐sprouter, but injury did not boost its RS ability. Root respiration did not differ between the two species and decreased continuously with time irrespectively of injury, but their phytohormone profiles differed significantly. In RS species, the auxins‐to‐cytokinins ratio was low, and injury further decreased it. Conclusions This first attempt to test drivers behind different plant growth forms suggests that intrinsic phytohormone regulation, especially the auxins‐to‐cytokinins ratio, might be behind RS ability. Injury, causing a phytohormonal imbalance, seems to be less important in spontaneously RS species than expected for RS species in general.

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The role of ABA in the freezing injury avoidance in two Hypericum species differing in frost tolerance and potential to synthesize hypericins

Cited by 20 publications

References 53 publications

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Effect of light conditions and ABA on cold storage and post-storage propagation of Taraxacum pieninicum

Conservation Strategies in the Genus Hypericum via Cryogenic Treatment

What determines root‐sprouting ability: Injury or phytohormones?

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