The role of active Bradyrhizobium japonicum in iron stress response of soybeans

Terry, Richard E.; Soerensen, K. U.; Jolley, D. Von; Brown, J. C.

doi:10.1007/978-94-011-3294-7_32

Cited by 19 publications

(23 citation statements)

References 12 publications

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“…The root systems of T203 soybean seedlings were split and grown in solution culture as described by Terry et al (1991). Four plants were grown in each split-root system.…”

Section: Growth and Inoculation Of Split-root Plantsmentioning

confidence: 99%

“…Symbiotic dinitrogen (N2) fixation in Bradyrhisobium-Mected legumes results in H + release into the rhizosphere and a reducing environment in the root nodule (Gibson, 1980). Both Fe-stress response and symbiotic N2 fixation occur in the root and are biochemical reduction processes which have been shown to interact, resulting in enhanced Fe-stress responses in Fe-efficient soybean (Soerensen et al, 1988 and1989;Terry et al, 1991) and peanut .…”

Section: Introductionmentioning

confidence: 98%

“…The soybean genotype T203 is Fe-inefficient and normally fails to produce any Fe-stress response; but in the absence of both N and Fe, T203 roots inoculated with Bradyrhizobium japonicum responded to Fe-deficiency stress by exuding both H + and reductants (Soerensen et al, 1988 and1989;Terry et al, 1991). In split-root experiments (Terry et al, 1991), nodulated soybean root systems embedded in agar containing a pH indicator produced H + mainly in association with active nodules.…”

Section: Introductionmentioning

confidence: 98%

“…In split-root experiments (Terry et al, 1991), nodulated soybean root systems embedded in agar containing a pH indicator produced H + mainly in association with active nodules. Reductant was exuded from young roots located just below the nodule clusters.…”

Section: Introductionmentioning

confidence: 99%

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Nitrogenase activity is required for the activation of iron‐stress response in iron‐inefficient T203 soybean

Terry

Jolley

1994

Journal of Plant Nutrition

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Iron (Fe)-stress response and symbiotic nitrogen (N 2 ) fixation are chemical reduction processes within the roots which interact to the extent that nodulated, Fe-stressed, Fe-inefficient soybean initiates Fe-stress response, but similar nonnodulated soybean does not. The objective of this study was to examine the relationship between nitrogenase activity and Fe-stress response in the Fe-inefficient T203 soybean by inoculating plants with effective or ineffective strains of Bradyrhizobium japonicum on either side of split-root systems. Half the roots of each plant were either inoculated with effective or ineffective strains or left uninoculated. Nodules formed on roots inoculated with the ineffective B. japonicum strain, SM5, were similar in number and shape to those on roots inoculated with the effective strain, 61A209, but roots inoculated with SM5 were free of nitrogenase activity. Hydrogen ion and reductant were not released from uninoculated roots or roots inoculated with SM5, but were released from active, effective nodules and the roots below the active nodule clusters of roots inoculated with effective B. japonicum. Plants with effective and ineffective strains inoculated on either half of the roots exhibited Fe-stress response only on the half with nitrogenase activity. Plants inoculated with both effective and ineffective strains, but provided nitrate to inhibit nitrogenase activity, lacked Fe-stress response and became severely Fe chlorotic. Iron reduction and hydrogen (H + ) release around nodules and roots of inoculated Fe-inefficient T203 soybean is keyed to nitrogenase activity.

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“…The root systems of T203 soybean seedlings were split and grown in solution culture as described by Terry et al (1991). Four plants were grown in each split-root system.…”

Section: Growth and Inoculation Of Split-root Plantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Nitrogenase activity is required for the activation of iron‐stress response in iron‐inefficient T203 soybean

Terry

Jolley

1994

Journal of Plant Nutrition

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“…Nodules have the highest ironconcentration of any plant tissue [37]. Nodule initiation and development, which are limited by iron deficiency [29,38], are coupled with enhanced root iron uptake [39]. Electron microscopy showed that the ferritin iron mineral in early nodules [5] was increased, as well as in the defective nodules of nematode-infected plants [ 15 ].…”

mentioning

confidence: 99%

Ferritin (mRNA, protein) and iron concentrations during soybean nodule development

Ragland

Theil

1993

Plant Mol Biol

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To study how iron-rich nodules concentrate and store iron, ferritin (mRNA, protein) was analyzed in developing soybean nodules and compared to nitrogenase (mRNA/activity) and leghemoglobin (mRNA, protein, heme). Both ferritin mRNA and protein concentrations increased early in nodulation. Later in nodulation ferritin protein declined, in contrast to the mRNA, as nitrogenase (mRNA and activity) increased and leghemoglobin (mRNA and protein) accumulated. A precursor/product relationship between iron stored in ferritin and iron in nitrogenase or leghemoglobin is suggested. The uncoordinated changes in ferritin mRNA and protein during nodulation contrast with nitrogenase mRNA and nitrogenase activity suggesting possible translational and posttranscriptional effects on ferritin expression.

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Iron and the Nodule

Guerinot

1993

Iron Chelation in Plants and Soil Microorganisms

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The role of active Bradyrhizobium japonicum in iron stress response of soybeans

Cited by 19 publications

References 12 publications

Nitrogenase activity is required for the activation of iron‐stress response in iron‐inefficient T203 soybean

Nitrogenase activity is required for the activation of iron‐stress response in iron‐inefficient T203 soybean

Ferritin (mRNA, protein) and iron concentrations during soybean nodule development

Iron and the Nodule

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