The Role of Adrenergic Stimulation in Regulation of Heat Production and Recruitment of Brown Adipose Tissue

Cannon, Barbara; Bengtsson, Tore; Dicker, Andrea; Jacobsson, Anders; Kuusela, Pertti; Thonberg, Håkan; Tvrdík, Petr; Zhao, Jin; Nedergaard, Jan

doi:10.1007/978-3-0348-7429-8_12

Cited by 10 publications

(10 citation statements)

References 23 publications

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“…Thus, in rats, periovarian adipose tissue contains brown cells reacting to cold similarly to IBAT. In this latter, the molecule responsible for the proliferation and activation of the tissue is noradrenaline (reviewed in Cannon et al, 1994), but NPY-, CGRP-and SP-containing nerves have also been identified . Here, we show that neuropeptide-containing nerves, already found in IBAT, also occur in periovarian brown fat with the same vascular and parenchymal distribution.…”

Section: Discussionmentioning

confidence: 98%

Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation

et al. 1996

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Rat periovarian adipose tissue contains unilocular adipocytes and some multilocular adipocytes that, following acclimation to cold, become more numerous and give rise to periovarian brown fat areas. We studied the occurrence and distribution of tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide, vasoactive intestinal peptide, methionine enkephalin, neurotensin, galanin, and cholecystokinin 9-20 in the nerves of rat periovarian tissue maintained at 20 degrees C (control rats), acclimated at 4 degrees C (cold acclimated rats) and at 28 degrees C (warm-acclimated rats). In the periovarian tissue of control and warm-acclimated rats, tyrosine hydroxylase-like, neuropeptide Y-like, substance P-like and calcitonin gene-related peptide-like immunoreactive elements (putative nerves) were present in the blood vessels. In the periovarian tissue of cold-acclimated rats, we found: (1) a more widespread vascular distribution of these neuropeptides; (2) tyrosine hydroxylase-like and calcitonin gene-related peptide-like immunoreactive elements among paucilocular and multilocular adipocytes (parenchymal-like nerves); (3) vasoactive intestinal peptide-like immunoreactive elements in some arteries. Investigation by EM showed the presence of heterogeneous non-myelinated axons both associated with capillaries and among paucilocular and multilocular adipocytes (parenchymal fibres) in periovarian brown fat areas. In conclusion, periovarian brown fat contains the same neuropeptides, with the same vascular and parenchymal distribution, already seen in typical depots of brown fat.

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Section: Discussionmentioning

confidence: 98%

Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation

et al. 1996

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“…Therefore, our results suggest that chronic noradrenaline stimulation does not change the b 1 /b 2 -AR binding capacity of BAT membranes. It could be argued, however, that whereas b 3 -AR are only present in mature brown adipocytes, b 1 /b 2 -AR are also present in preadipocytes (13) and that preadipocyte number might have increased during cold-acclimation, thus masking a reduction in binding density. However, Bukowiecki et al (14) showed that in the rat at room temperature the ratio of mature adipocytes to preadipocytes is 40:2, and, most importantly, those proportions are unchanged after 15 days in the cold.…”

Section: Discussionmentioning

confidence: 99%

Oestradiol and progesterone change beta3-adrenergic receptor affinity and density in brown adipocytes

Malo

Puerta²

2001

European Journal of Endocrinology

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Objective: To check if the oestradiol-and progesterone-driven reduction in noradrenaline responsiveness of brown adipocytes is due to a reduction in either the density or the affinity of b 3 -adrenoceptors (b 3 -AR). b 1 /b 2 -AR were also studied. Design: Four groups of animals were considered. (i) control rats at thermoneutrality, (ii) coldacclimated rats, to determine b-AR under continuous sympathetic stimulation, which is known to decrease noradrenaline responsiveness, (iii) oestradiol-and (iv) progesterone-treated cold-acclimated rats to determine hormonal effects on b-AR populations in thermogenically active brown adipocytes. Methods: Oestradiol and progesterone were chronically elevated by means of s.c. Silastic implants. Densities and affinities of b-AR populations were determined by binding studies using

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“…Changes in cell number, in their content of mitochondrial proteins, and in the concentration of uncoupling protein in the mitochondria account for growth and atrophy of BAT in many physiological and pathological conditions (Himms-Hagen 1989, 1990. In rats, changes in sympathetic activity correlate well with changes in thermogenic capacity, and norepinephrine exerts both thermogenic and trophic influences (Himms-Hagen 1990;Cannon et al 1994).…”

Section: Introductionmentioning

confidence: 96%

Expressed α₁-adrenoceptors in adult rat brown adipocytes are primarily of α_1Asubtype

Costain¹,

Mainra²,

Desautels³

et al. 1996

Can. J. Physiol. Pharmacol.

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The main objective of this study was to characterize the alpha 1-adrenoceptors expressed in adult rat brown adipocytes. For this purpose, membrane fractions were prepared from brown adipose tissue as well as from isolated brown adipocytes. The following are major findings: (i) BAT membranes were considerably enriched in alpha 1-adrenoceptors (specific [3H]prazosin binding, Bmax, 79.49 +/- 16.77 fmol/mg protein; KD, 0.24 +/- 0.04 nM); (ii) among the cells that comprise brown adipose tissue, brown adipocytes were enriched in alpha 1-adrenoceptors; (iii) > 95% of total alpha 1-adrenoceptors were resistant to inactivation by 20 microM chloroethylclonidine, which readily and essentially completely inactivated alpha 1B-adrenoceptors in rat liver membranes; (iv) brown adipose tissue membrane alpha 1-adrenoceptors showed high affinity towards 5-methyl urapidil (KD 7.23 +/- 2.49 nM) and WB 4101 (KD 0.66 +/- 0.30 nM) and low affinity towards BMY 7378 (KD 0.34 +/- 0.03 microM); essentially similar affinities for these drugs were seen for membranes prepared from brown adipocytes; and (v) EBDA/LIGAND analysis of 5-methyl urapidil, WB 4101, and BMY 7378 competition curves revealed the presence of a single binding site for these drugs. Recent work has documented that 5-methyl urapidil and WB 4101 interact with high affinity with alpha 1A-adrenoceptors, while BMY 7378 interacts with high affinity with alpha 1D-adrenoceptors. Taken together, these findings are consistent with the view that alpha 1-adrenoceptors expressed in adult rat BAT are mainly of the alpha 1A subtype.

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The Role of Adrenergic Stimulation in Regulation of Heat Production and Recruitment of Brown Adipose Tissue

Cited by 10 publications

References 23 publications

Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation

Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation

Oestradiol and progesterone change beta3-adrenergic receptor affinity and density in brown adipocytes

Expressed α₁-adrenoceptors in adult rat brown adipocytes are primarily of α_1Asubtype

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The Role of Adrenergic Stimulation in Regulation of Heat Production and Recruitment of Brown Adipose Tissue

Cited by 10 publications

References 23 publications

Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation

Tyrosine hydroxylase, neuropeptide Y, substance P, calcitonin gene-related peptide and vasoactive intestinal peptide in nerves of rat periovarian adipose tissue: an immunohistochemical and ultrastructural investigation

Oestradiol and progesterone change beta3-adrenergic receptor affinity and density in brown adipocytes

Expressed α1-adrenoceptors in adult rat brown adipocytes are primarily of α1Asubtype

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Expressed α₁-adrenoceptors in adult rat brown adipocytes are primarily of α_1Asubtype