The role of adrenomedullin and its receptor system in cardiovascular calcification of rat induced by Vitamin D3 plus nicotine

Pan, Chun‐Shui; Qi, Yongfen; Wu, Sheng Ying; Li, G.Z; Tang, Chaoshu

doi:10.1016/j.peptides.2004.02.020

Cited by 13 publications

(4 citation statements)

References 31 publications

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“…From prior studies an association between this marker and the CACS would have been expected [6,23]. However, we did not find an association between MR-proADM and the CACS.…”

Section: Discussioncontrasting

confidence: 76%

“…The difference(s) in design between the existing studies and ours may account for the controversial findings. Whereas in other studies the calcification process was investigated in vitro and in animal models (rats) being fed with vitamin D and nicotine [6,23], the present study was performed in subjects with normal renal function and balanced calciumphosphate homeostasis. Another difference is the technique applied for the evaluation of calcification processes, which was atomic absorption spectrophotometry in invitro studies [6,21] and 64-MSCT imaging in our study, which is the gold standard technique for the non-invasive evaluation of the CACS in various clinical settings [24][25][26].…”

Section: Discussionmentioning

confidence: 99%

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Association of midregional proadrenomedullin with coronary artery stenoses, soft atherosclerotic plaques and coronary artery calcium

et al. 2011

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Midregional proadrenomedullin (MR-proADM) is elevated in patients with heart failure and myocardial infarction. The aim of this study was to evaluate the association of MR-proADM with the grade of coronary artery stenosis, presence of coronary artery soft plaques and coronary artery calcification score (CACS), determined by 64-multislice computed tomography (MSCT) in patients without known prior cardiovascular disease. This retrospective study included 107 patients undergoing MSCT for confirmation (or exclusion) of coronary artery disease. MR-proADM levels were measured in all patients. The assessment of coronary artery stenoses, CACS and soft coronary plaques was made by MSCT using known criteria. The MR-proADM [median (25th-75th percentiles)] level was 0.33 (0.21-0.43) nmol/l. The MR-proADM level was 0.28 (0.22-0.40) nmol/l in patients with coronary stenoses ≥50% (n = 23) versus 0.33 (0.27-0.40) nmol/l in patients with coronary stenoses <50% (n = 83, P = 0.59), 0.33 (0.26-0.40) nmol/l in patients with soft plaques (n = 56) versus 0.33 (0.25-0.41) nmol/l in patients without soft plaques (n = 50, P = 0.73) and 0.33 (0.25-0.39) nmol/l in patients with CACS <200 (n = 81) versus 0.32 (0.26-0.44) nmol/l in patients with CACS ≥200 (n = 26, P = 0.77). In multivariate analysis, the MR-proADM level was a significant correlate of coronary artery stenoses [odds ratio (OR) = 0.93; 95% confidence interval (CI) 0.86-0.99; P = 0.026] and soft plaques (OR = 0.94; 95% CI 0.90-0.99; P = 0.015) but not of CACS (OR = 0.98; 95% CI 0.93-1.03; P = 0.36). A decreased MR-proADM level is an independent correlate of the presence of coronary artery disease and of soft atherosclerotic plaques. Patients with decreased MR-proADM levels may need invasive examinations to diagnose more severe forms of coronary artery disease.

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“…From prior studies an association between this marker and the CACS would have been expected [6,23]. However, we did not find an association between MR-proADM and the CACS.…”

Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Association of midregional proadrenomedullin with coronary artery stenoses, soft atherosclerotic plaques and coronary artery calcium

et al. 2011

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“…Total myocardial and aortic RNA was prepared by in situ lysis with trizol reagent (Pan et al, 2004). One microgram of total tissue RNA was reverse transcribed into a single-strand cDNA with M-MuLV reverse transcriptase and oligo(dT) 15 primers.…”

Section: Characteristics Of Secreted Admmentioning

confidence: 99%

Hypertension induced by nitric oxide synthase inhibitor increases responsiveness of ventricular myocardium and aorta of rat tissue to adrenomedullin stimulation in vitro

et al. 2005

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“…It was ever reported that hHSCs expressed the receptor CRLR for AM and their associated proteins Receptor activity modifying protein (RAMP)‐1 and Receptor activity modifying protein (RAMP)‐2 in addition to spontaneously secreting AM (Gorbig et al . 2001; Pan et al . 2004).…”

Section: Discussionmentioning

confidence: 99%

Adrenomedullin regulates expressions of transforming growth factor‐β1 and β1‐induced matrix metalloproteinase‐2 in hepatic stellate cells

Wang

Zhang

Qian

et al. 2006

Int J Experimental Path

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Adrenomedullin (AM), a peptide isolated from human pheochromocytoma, can be produced and secreted by various types of cells including hepatic stellate cells (HSCs), and its possible role in HSCs is not clear now. In the present study, the interactive regulation between transforming growth factor (TGF)-beta1 and AM and the effect of AM on TGF-beta1-induced matrix metalloproteinase (MMP)-2 expression in HSCs were investigated. TGF-beta1 and AM inhibited gene transcript level mutually (real-time reverse transcription-polymerase chain reaction). AM suppressed the protein expression level of TGF-beta1 (Western blot), but TGF-beta1 might have no effect on AM secretion level. MMP-2 protein expression in HSCs was increased in response to TGF-beta1, and upregulation of MMP-2 expression stimulated with TGF-beta1 was suppressed by AM in dose-dependent manner (Western blot). AM decreased the phosphorylation level of extracellular signal-regulated kinase (ERK) in HSCs treated with TGF-beta1, and TGF-beta1-induced MMP-2 expression was suppressed by adding Mitogen-activated protein Kinase/ERK (MEK) inhibitor U(0126) (Western blot)(.) Our results suggest that AM may intervene the activation of HSCs by inhibiting TGF-beta1 production and TGF-beta1-induced MMP-2 expression; AM may suppress the upregulation of MMP-2 expression induced by TGF-beta1 partially through ERK pathway.

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The role of adrenomedullin and its receptor system in cardiovascular calcification of rat induced by Vitamin D3 plus nicotine

Cited by 13 publications

References 31 publications

Association of midregional proadrenomedullin with coronary artery stenoses, soft atherosclerotic plaques and coronary artery calcium

Association of midregional proadrenomedullin with coronary artery stenoses, soft atherosclerotic plaques and coronary artery calcium

Hypertension induced by nitric oxide synthase inhibitor increases responsiveness of ventricular myocardium and aorta of rat tissue to adrenomedullin stimulation in vitro

Adrenomedullin regulates expressions of transforming growth factor‐β1 and β1‐induced matrix metalloproteinase‐2 in hepatic stellate cells

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