The role of androgen metabolism in the control of androgen action in the rat prostate

Tenniswood, Martin; Bird, C. E.; Clark, A. F.

doi:10.1016/0303-7207(82)90065-x

Cited by 19 publications

(8 citation statements)

References 42 publications

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“…In itself this is not particularly surprising since the synthesis of secretory products, such as PSBP and SAP, is known to be reduced or eliminated in replicating cells [28,29]. However, it seems unlikely that the only difference between the RDE cells and the normal androgen-dependent epithelial cells is the presence or absence of the secretory processes, for two reasons.…”

Section: Discussionmentioning

confidence: 99%

Androgen‐independent epithelial cells of the rat ventral prostate

et al. 1988

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Androgen-independent cell lines have been clonally selected from primary cultures of androgen-dependent epithelial cells from the rat ventral prostate. These rapidly dividing epithelial-like cells (RDE) have altered morphology and adherence characteristics. Unlike normal prostate epithelial cells, the RDE cell lines do not require androgens for cell division or cell survival. In the presence of physiological concentrations of testosterone, the isoelectric focusing patterns of prostatic acid phosphatases are abnormal in these RDE cells, and the prostate steroid-binding protein genes are not expressed. The loss of androgen dependence is not due to the inability of RDE cells to metabolize testosterone to 5 alpha-dihydrotestosterone, the active androgen, since the RDE cell lines metabolize testosterone in a manner similar to normal androgen-dependent epithelial cells. When RDE cells are grown on collagen matrices, the cells assume ductlike structures, similar to prostatic acini, although PSBP gene expression is not induced. When seeded into soft agar these cell lines form distinct foci, suggesting that they are potentially tumorigenic.

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Section: Discussionmentioning

confidence: 99%

Androgen‐independent epithelial cells of the rat ventral prostate

et al. 1988

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“…However, 5a-dihydrotestosterone at doses with equivalent androgenic activity was without significant effect. Since, for the rat prostate at least, it has been shown that the active species at the level of the androgen receptor is 5a-dihydrotestosterone formed in situ from testosterone by the action of 5a-reductase (Tenniswood, Bird & Clark, 1982) (Humphrey, 1968), and testosterone propionate administered on Days 3 and 4 results in a delay, while oestradiol administration after embryos have entered the isthmus results in rapid expulsion and loss of embryos (Lee, 1979 (Humphrey, 1968). The results therefore suggest that the delay caused by administration of testosterone is anti¬ progestational in nature.…”

Section: Discussionmentioning

confidence: 99%

Further investigation of the role of progesterone in the control of embryo transport in the mouse

Grieve¹,

Henwood²,

Kendle³

1985

Reproduction

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“…The secretory epithelial cells of the rat prostate require androgens for survival [Tenniswood et al, 1982;Isaacs, 1984]. Following androgen ablation by either surgical or chemical castration, the epithelial cells of the prostate undergo successive waves of apoptosis, resulting in a dramatic decrease in cell number [Bruchovsky et al, 1975;Lee, 1981].…”

Section: Introductionmentioning

confidence: 99%

Embigin, a developmentally expressed member of the immunoglobulin super family, is also expressed during regression of prostate and mammary gland

et al. 1997

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Cell adhesion molecules (CAMs) are intimately involved in a variety of cellular processes, including development, cell growth, apoptosis, and differentiation. Interaction of CAMs with components of the extracellular matrix (ECM), growth factors, and other CAMs provides an intricate regulatory mechanism for a diverse range of cellular responses. Embigin is a developmentally expressed protein that is a member of the immunoglobulin superfamily (IgSF) class of CAMs. We have identified embigin as a gene expressed during tissue regression in rat prostate and lactating mammary gland following hormonal ablation. In the absence of the appropriate hormone, the secretory epithelial cells of these two tissues undergo successive waves of apoptotic cell death co‐incident with extensive reorganization of the surviving tissue. Using Northern analysis, in situ hybridization analysis, RT‐PCR, and Western analysis, we have characterized the expression of embigin mRNA and protein in both regressing prostate and mammary gland. During development of the prostate gland, increased expression of embigin is correlated with the appearance of highly organized lumenal and ductal structures. Embigin is also expressed in a variety of adult tissues including heart, liver, lung, and brain. Zoo‐blot analysis with the rat embigin cDNA indicates that embigin homologs exist in species as diverse as Homo sapiens and Drosophila melanogaster, suggesting that it has been highly conserved during evolution. Embigin protein is expressed at readily detectable levels in a variety of prostate and mammary cancer cell lines, and in some cell lines the expression of embigin appears to be down‐regulated in the presence of ECM. Our data have led us to propose a model in which embigin functions as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. Dev. Genet. 21:268–278, 1997. © 1997 Wiley‐Liss, Inc.

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The role of androgen metabolism in the control of androgen action in the rat prostate

Cited by 19 publications

References 42 publications

Androgen‐independent epithelial cells of the rat ventral prostate

Androgen‐independent epithelial cells of the rat ventral prostate

Further investigation of the role of progesterone in the control of embryo transport in the mouse

Embigin, a developmentally expressed member of the immunoglobulin super family, is also expressed during regression of prostate and mammary gland

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