The role of antioxidants in sperm freezing: a review

Amidi, Fardin; Pazhohan, Azar; Nashtaei, Maryam Shabani; Khodarahmian, Mahshad; Nekoonam, Saeid

doi:10.1007/s10561-016-9566-5

Cited by 249 publications

(177 citation statements)

References 122 publications

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“…Unlike synthetic chemicals, biomaterials such as alginates, polyvinyl alcohol, and chitosan can be used to impede ice crystal growth, along with traditional small molecules 4 . The direct inhibition of ice crystal formation and application of antioxidants and other compounds have been used to attempt to reduce cell death from processes such as apoptosis during the freezing and thawing cycle 20, 21, 22, 23. Common CPAs are briefly addressed in the following subsections and Table 2.…”

Section: Cryopreservationmentioning

confidence: 99%

Cryopreservation and its clinical applications

Jang

Park

Yang

et al. 2017

Integrative Medicine Research

361

234

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Cryopreservation is a process that preserves organelles, cells, tissues, or any other biological constructs by cooling the samples to very low temperatures. The responses of living cells to ice formation are of theoretical interest and practical relevance. Stem cells and other viable tissues, which have great potential for use in basic research as well as for many medical applications, cannot be stored with simple cooling or freezing for a long time because ice crystal formation, osmotic shock, and membrane damage during freezing and thawing will cause cell death. The successful cryopreservation of cells and tissues has been gradually increasing in recent years, with the use of cryoprotective agents and temperature control equipment. Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. In this review, we briefly address representative cryopreservation processes, such as slow freezing and vitrification, and the available cryoprotective agents. In addition, some adverse effects of cryopreservation are mentioned.

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Section: Cryopreservationmentioning

confidence: 99%

Cryopreservation and its clinical applications

Jang

Park

Yang

et al. 2017

Integrative Medicine Research

361

234

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“…They reported the protective effect of antioxidants against ROS production on human, goat, ram, bull, canine and boar sperm quality. However, they concluded that more research is warranted to a draw conclusive evidence regarding the use of antioxidants in a clinical setting (Amidi, Pazhohan, Nashtaei, Khodarahmian, & Nekoonam, 2016).…”

mentioning

confidence: 99%

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

et al. 2020

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To investigate the impact of antioxidants in sperm parameters and reduction in reactive oxygen species production during the freeze‐thaw process. PubMed, Scopus, Web of Science, Embase and Cochrane central library were systematically searched. Of the 1583 articles, 23 studies were selected for data extraction. Our results show that antioxidants improved sperm progressive motility (standardised mean difference (SMD) = 1; 95% CI: 0.62, 1.38; p < .001) and viability (SMD = 1.20; 95% CI: 0.50, 1.91; p = .001) and reduced sperm DNA fragmentation (SDF) and hydrogen peroxide (H2O2) production, but there was no significant improvement in total sperm motility after thawing. Acetyl‐l‐carnitine/l‐carnitine, melatonin and catalase had a significant positive impact on progressive motility. The role of tempol and melatonin in improving viability was significant compared to other antioxidants. Moreover, a significant reduction in SDF was observed after addition of butylated hydroxytoluene, tempol and vitamin E. However, the prevention of H2O2 production was significant only after the addition of tempol. Our overall results displayed the positive impact of antioxidants on progressive sperm motility, viability and reduction in SDF and H2O2 production, but no significant impact of antioxidants on total sperm motility was seen during the freeze‐thaw process.

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“…Research in cryopreservation of oocytes, embryos and sperm (Amidi et al, 2016;Kopeika et al, 2015) suggest that shearing of DNA during freeze-thaw can be reduced by the addition of antioxidants, indicating that oxidation of DNA by radicals may, at least in vivo, be a significant cause of DNA fragmentation. Such conclusions must however be considered highly tentative as there are substantial differences in the chemical environment inside the cell compared to the in vitro conditions covered by this study.…”

Section: Freezing and Long-term Storagementioning

confidence: 99%

A comprehensive model of DNA fragmentation for the preservation of High Molecular Weight DNA

Klingström

Bongcam‐Rudloff

2018

Preprint

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During DNA extraction the DNA molecule undergoes physical and chemical shearing, causing the DNA to fragment into shorter and shorter pieces. Under common laboratory conditions this fragmentation yields DNA fragments of 5-35 kilobases (kb) in length. This fragment length is more than sufficient for DNA sequencing using short-read technologies which generate reads 50-600 bp in length, but insufficient for long-read sequencing and linked reads where fragment lengths of more than 40 kb may be desirable.This study provides a theoretical framework for quality management to ensure access to high molecular weight DNA in samples. Shearing can be divided into physical and chemical shearing which generate different patterns of fragmentation. Exposure to physical shearing creates a characteristic fragment length where DNA fragments are cut in half by shear stress. This characteristic length can be measured using gel electrophoresis or instruments for DNA fragment analysis. Chemical shearing generates randomly distributed fragment lengths visible as a smear of DNA below the peak fragment length. By measuring the peak of DNA fragment length and the proportion of very short DNA fragments both sources of shearing can be measured using commonly used laboratory techniques, providing a suitable quantification of DNA integrity of DNA for sequencing with long-read technologies.

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The role of antioxidants in sperm freezing: a review

Cited by 249 publications

References 122 publications

Cryopreservation and its clinical applications

Cryopreservation and its clinical applications

The efficacy of antioxidants in sperm parameters and production of reactive oxygen species levels during the freeze‐thaw process: A systematic review and meta‐analysis

A comprehensive model of DNA fragmentation for the preservation of High Molecular Weight DNA

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