The role of Astragalus polysaccharides in promoting IEC-6 cell migration from polyamine-mediated Ca2+ regulation

Zhang, Dong; Zhu, Yiping; Li, Zhijin; Luo, Meng; Liang, Xiaofang; Wang, Anrong; Zhu, Hua; Hu, Ling; Li, Ru-Liu

doi:10.1016/j.ijbiomac.2022.02.109

Cited by 8 publications

(3 citation statements)

References 17 publications

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“…There are researches showed that knocking out the canonical transient receptor potential channel 1 (TRPC1) can decrease Ca + influx and ROS production, thereby exerting a protective effect on the heart. , The natural compound chlorogenic acid has been demonstrated to protect endothelial cells from LPC-induced injury by attenuating TRPC1 expression . Furthermore, the polysaccharides in Hedysarum Multijugum Maxim and Radix Salviae also exhibit inhibitory effects on TRPC1, , providing new directions for research in this field.…”

Section: Introductionmentioning

confidence: 99%

“… 4 , 5 The natural compound chlorogenic acid has been demonstrated to protect endothelial cells from LPC-induced injury by attenuating TRPC1 expression. 6 Furthermore, the polysaccharides in Hedysarum Multijugum Maxim and Radix Salviae also exhibit inhibitory effects on TRPC1, 7 , 8 providing new directions for research in this field.…”

Section: Introductionmentioning

confidence: 99%

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Exploring the Potential Mechanisms of Guanxinshutong Capsules in Treating Pathological Cardiac Hypertrophy based on Network Pharmacology, Computer-Aided Drug Design, and Animal Experiments

Liu,

Li,

Shao

et al. 2024

ACS Omega

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Cardiovascular diseases (CVDs) are significant causes of morbidity and mortality worldwide, and pathological cardiac hypertrophy (PCH) is an essential predictor of many heart diseases. Guanxinshutong capsule (GXST) is a Chinese patent medicine widely used in the clinical treatment of CVD, In our previous research, we identified 111 compounds of GXST. In order to reveal the potential molecular mechanisms by which GXST treats PCH, this study employed network pharmacology methods to screen for the active ingredients of GXST in treating PCH and predicted the potential targets. The results identified 26 active ingredients of GXST and 110 potential targets for PCH. Through a protein−protein interaction (PPI) network, gene ontology (GO), and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, we confirmed AKT1, MAPK1, and MAPK3 as the core proteins in GXST treatment of PCH, thus establishing the PI3K/ AKT and MAPK signaling pathways as the significant mechanisms of GXST in treating PCH. The results of molecular docking (MD) demonstrate that flavonoid naringenin and diterpenoid tanshinone iia have the highest binding affinity with the core protein.Before performing molecular dynamics simulations (MDSs), the geometric structure of naringenin and tanshinone iia was optimized using density functional theory (DFT) at the B97−3c level, and RESP2 atomic charge calculations were carried out at the B3LYP-D3(BJ)/def2-TZVP level. Further MDS results demonstrated that in the human body environment, the complex of naringenin and tanshinone iii with core proteins exhibited high stability, flexibility, and low binding free energy. Additionally, naringenin and tanshinone iia showed favorable absorption, distribution, metabolism, excretion, and toxicity (ADMET) characteristics and passed the drug similarity (DS) assessment. Ultrasound cardiograms and cardiac morphometric measurements in animal experiments demonstrate that GXST can improve the PCH induced by isoproterenol (ISO). Protein immunoblotting results indicate that GXST increases the expression of P-eNOS and eNOS by activating the PI3K/AKT signaling pathway and the MAPK signaling pathway, further elucidating the mechanism of action of GXST in treating PCH. This study contributes to the elucidation of the key ingredients and molecular mechanisms of GXST in treating PCH.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Exploring the Potential Mechanisms of Guanxinshutong Capsules in Treating Pathological Cardiac Hypertrophy based on Network Pharmacology, Computer-Aided Drug Design, and Animal Experiments

Liu,

Li,

Shao

et al. 2024

ACS Omega

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“…Previous research has shown that APS alleviates intestinal inflammation in broilers by regulating intestinal immunity, helper T cell 17/regulatory T cell balance, and the cecal microbiome 7 . In addition, APS used in the treatment of gastrointestinal mucosal injury promotes intestinal cell migration through the polyamine calcium signaling pathway 8 . Furthermore, Salvia miltiorrhiza polysaccharide (SMP) is an effective component of S. miltiorrhiza , which has been widely studied for its pharmacological effects, such as antioxidant function, immune regulation, and liver protection 9 .…”

Section: Introductionmentioning

confidence: 99%

Effects of three different plant‐derived polysaccharides on growth performance, immunity, antioxidant function, and cecal microbiota of broilers

Zhao,

Niu,

Wang

et al. 2023

J Sci Food Agric

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BackgroundThis study investigated the effects of dietary plant polysaccharides on growth performance, immune status and intestinal health in broilers. We randomly divided 960 one‐day‐old Arbor Acres broiler chicks into 4 groups. The control (CON) group was fed a basal diet, and the remaining groups were fed a basal diet supplemented with 1,000 mg/kg ginseng polysaccharide (GPS), astragalus polysaccharides (APS), or Salvia miltiorrhiza polysaccharide (SMP) for 42 d.ResultsDietary supplementation with SMP significantly increased BW at 21 and 42 days of age, ADG and ADFI during the starter and whole experimental period, decreased the concentrations of IL‐1β, TNF‐α and MDA, increased the levels of IL‐4 and IL‐10 and CAT activity in the serum (P < 0.05). GPS, APS, and SMP supplementation increased serum levels of immunoglobulins, activities of GSH‐Px, T‐SOD and T‐AOC, and cecal concentrations of acetic acid and propionic acid of broilers (P < 0.05). Furthermore, high‐throughput sequencing results showed that the relative abundance of Firmicutes was decreased while the relative abundance of Bacteroidota, Alistipes, and Prevotellaceae_NK3B31_group were increased (P < 0.05) in the GPS, APS, and GMP groups compared with the CON group.ConclusionDietary GPS, APS, and SMP supplementation could improve growth performance, enhance immune function by increasing serum immunoglobulin and regulating cytokines, improve antioxidant function by increasing serum antioxidant enzyme activity, increase volatile fatty acid levels and improve the microbial composition in the cecum of broilers. Dietary SMP supplementation had the optimal effect in this study.This article is protected by copyright. All rights reserved.

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MYO3B promotes cancer progression in endometrial cancer by mediating the calcium ion-RhoA/ROCK1 signaling pathway

Zhang,

Yang

et al. 2024

J Cancer Res Clin Oncol

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The role of Astragalus polysaccharides in promoting IEC-6 cell migration from polyamine-mediated Ca2+ regulation

Cited by 8 publications

References 17 publications

Exploring the Potential Mechanisms of Guanxinshutong Capsules in Treating Pathological Cardiac Hypertrophy based on Network Pharmacology, Computer-Aided Drug Design, and Animal Experiments

Exploring the Potential Mechanisms of Guanxinshutong Capsules in Treating Pathological Cardiac Hypertrophy based on Network Pharmacology, Computer-Aided Drug Design, and Animal Experiments

Effects of three different plant‐derived polysaccharides on growth performance, immunity, antioxidant function, and cecal microbiota of broilers

MYO3B promotes cancer progression in endometrial cancer by mediating the calcium ion-RhoA/ROCK1 signaling pathway

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