The Role of Bacterial Biofilms in Chronic Infectious Processes and the Search for Methods to Combat Them

Ilyina, T. S.; Romanova, Yu. M.

doi:10.3103/s0891416821020026

Cited by 6 publications

(2 citation statements)

References 83 publications

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Section: Biofilmsmentioning

confidence: 99%

“…Nosocomial infections frequently involve biofilms on tissue and medical devices ( 6 – 8 ). Biofilms are often associated with antibiotic resistance, promoting chronic and persistent infections, which make them difficult to treat ( 6 – 8 ). Microbial biofilm formation is complex, involving signaling and regulatory pathways that control the transition from motile to sessile lifestyle, metabolic status, cell coordination, production of an extracellular polymeric matrix, and maturation of the biofilm’s 3D structure.…”

Section: Biofilmsmentioning

confidence: 99%

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Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Gager,

Flores-Mireles

2024

mSphere

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The spread of multi-drug-resistant (MDR) pathogens has rapidly outpaced the development of effective treatments. Diverse resistance mechanisms further limit the effectiveness of our best treatments, including multi-drug regimens and last line-of-defense antimicrobials. Biofilm formation is a powerful component of microbial pathogenesis, providing a scaffold for efficient colonization and shielding against anti-microbials, which further complicates drug resistance studies. Early genetic knockout tools didn’t allow the study of essential genes, but clustered regularly interspaced palindromic repeat inference (CRISPRi) technologies have overcome this challenge via genetic silencing. These tools rapidly evolved to meet new demands and exploit native CRISPR systems. Modern tools range from the creation of massive CRISPRi libraries to tunable modulation of gene expression with CRISPR activation (CRISPRa). This review discusses the rapid expansion of CRISPRi/a-based technologies, their use in investigating MDR and biofilm formation, and how this drives further development of a potent tool to comprehensively examine multi-drug resistance.

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Section: Biofilmsmentioning

confidence: 99%

Section: Biofilmsmentioning

confidence: 99%

Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Gager,

Flores-Mireles

2024

mSphere

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Surface modification of polyester films with polyfunctional amines: Effect on bacterial biofilm formation

Mallamaci

Brugnoli

Mariano

et al. 2023

Surfaces and Interfaces

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Investigating the antimicrobial and antibiofilm properties of marine halophilic Bacillus species against ESKAPE pathogens

Murphy,

Culligan,

Murphy

2024

Environ Microbiol Rep

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Antimicrobial resistance (AMR), known as the “silent pandemic,” is exacerbated by pathogenic bacteria's ability to form biofilms. Marine compounds hold promise for novel antibacterial drug discovery. Two isolates from preliminary saltwater environment screening demonstrated antimicrobial activity and were subsequently identified as Bacillus subtilis MTUA2 and Bacillus velezensis MTUC2. Minimum inhibitory concentrations (MICs), minimum biofilm inhibition concentrations (MBICs) and minimum biofilm eradication concentrations (MBECs) required to prevent and/or disrupt bacterial growth and biofilm formation were established for MRSA, Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli. The metabolic activity within biofilms was determined by the 2,3,5‐triphenyltetrazolium chloride assay. Both Bacillus species exhibited unique antimicrobial effects, reducing MRSA and S. aureus planktonic cell growth by 50% and sessile cell growth for S. aureus and E. coli by 50% and 90%, respectively. No effect was observed against A. baumannii. Significant MBIC and MBEC values were achieved, with 99% inhibition and 90% reduction in MRSA and S. aureus biofilms. Additionally, 90% and 50% inhibition was observed in E. coli and A. baumannii biofilms, respectively, with a 50% reduction in E. coli biofilm. These findings suggest that the mode of action employed by B. subtilis MTUA2 and B. velezensis MTUC2 metabolites should be further characterized and could be beneficial if used independently or in combination with other treatments.

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The Role of Bacterial Biofilms in Chronic Infectious Processes and the Search for Methods to Combat Them

Cited by 6 publications

References 83 publications

Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Blunted blades: new CRISPR-derived technologies to dissect microbial multi-drug resistance and biofilm formation

Surface modification of polyester films with polyfunctional amines: Effect on bacterial biofilm formation

Investigating the antimicrobial and antibiofilm properties of marine halophilic Bacillus species against ESKAPE pathogens

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