The role of Bax in the apoptosis of Leishmania-infected macrophages

Aghaei, Mohammad; Khanahmad, Hossein; Aghaei, Shahrzad; Nilforoushzadeh, Mohammad Ali; Mohaghegh, Mohammad Ali; Hejazi‬, ‪Seyed Hossein

doi:10.1016/j.micpath.2019.103892

Cited by 19 publications

(6 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the protein levels of Ki67 and PCNA, two main proliferation-related proteins (27), were also suppressed by lincRNA-Cox2. Apoptosis is an important process associated with cell viability, and the activation of Bax, c-Caspase 3 and c-Caspase 9 are responsible for regulating apoptosis (28,29). The present study demonstrated that both the rate of apoptosis and expression of Bax, c-Caspase 3 and c-Caspase 9 were reduced after knockdown of lincRNA-Cox2, suggesting an important pro-apoptotic role of lincRNA-Cox2 in OA chondrocytes.…”

Section: Discussionsupporting

confidence: 57%

Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

Jiang

Ren

et al. 2021

Exp Ther Med

View full text Add to dashboard Cite

Osteoarthritis (OA) is a joint disease characterised by progressive cartilage degradation and inflammation, but the detailed pathogenesis of OA remains unclear. The present study aimed to investigate the role of long intergenic non-coding RNA (lincRNA)-Cox2 in OA progression and the potential mechanism. An OA mouse model was used for in vivo experiments, and IL-1β-induced injury of mouse chondrocytes was conducted for in vitro experiments. Small interfering (si)-Cox2 was transfected into chondrocytes to elucidate the effect of lincRNA-Cox2 on OA. Quantitative reverse transcription PCR assays were conducted to detect the expression of lincRNA-Cox2 and microRNA (miR)-150. Cell proliferation and apoptosis were analysed based on an MTT assay and annexin V/propidium iodide staining, respectively. Western blotting was performed to evaluate the protein expression levels of Ki-67, PCNA, Bax, cleaved (c)-Caspase-3, c-Caspase-9 and Wnt/β-catenin pathway-associated proteins in chondrocytes. High levels of lincRNA-Cox2 were observed in cartilage tissues of the OA mouse model in vivo. In the in vitro experiments, the expression of lincRNA-Cox2 was increased in IL-1β-treated chondrocytes. Knockdown of lincRNA-Cox2 promoted the proliferation and inhibited the apoptosis of chondrocytes. Mechanistically, lincRNA-Cox2 was found to directly target miR-150, acting as a competing endogenous RNA, and the effect of si-Cox2 on the proliferation and apoptosis of chondrocytes was reversed by miR-150 inhibitors. Moreover, lincRNA-Cox2 activated the Wnt/β-catenin pathway to regulate chondrocyte proliferation and apoptosis. The present study demonstrated that silencing lincRNA-Cox2 expression plays a protective role in OA by enhancing the proliferation and suppressing the apoptosis of chondrocytes, which is related to increased miR-150 expression and activation of the Wnt/β-catenin pathway.

show abstract

Section: Discussionsupporting

confidence: 57%

Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

Jiang

Ren

et al. 2021

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…In addition, the protein levels of Ki67 and PCNA, two main proliferation related proteins, were also suppressed by LincRNA-Cox2. Apoptosis is an important processes associated cell viability, and activation of Bax, c-Caspase 3 and c-Caspase 9 are responsible for monitor of cell apoptosis [25,1]. In this study, we found that both the apoptosis cells and the expression of Bax, c-Caspase 3 and c-Caspase 9 are reduced after knockdown of LincRNA-Cox2, suggesting an important pro-apoptotic role of LincRNA-Cox2 in OA chondrocytes.…”

Section: Deficiency Of Mir-150 Reverses the Effect Of Lincrna-cox2 Knsupporting

confidence: 50%

LincRNA-Cox2 targeting miR-150 regulating the proliferation and apoptosis of chondrocytes in osteoarthritis

Jiang

Ren

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Osteoarthritis (OA) is a joint disease characterized by progressive cartilage degradation and inflammation, but the detailed pathogenesis of OA is still unclear. Here, we aimed to investigate the role of LincRNA-Cox2 in OA progression and the potential mechanism.Methods: OA mouse model and IL-1β-induced injury of mouse chondrocytes were conducted. Si-Cox2 was transfected into chondrocytes for elucidating the effect of LincRNA-Cox2 on OA. qR-TPCR was used to detect the expression of LincRNA-Cox2 and miR-150. Cell proliferation and apoptosis were analyzed by MTT assay and Annexin V/PI stain respectively. Western blot was used to evaluate the protein levels in chondrocytes.Results: High levels of LincRNA-Cox2 were observed in both cartilage tissues of OA and IL-1β-treated chondrocytes. Knockdown of LincRNA-Cox2 promoted the proliferation and inhibited apoptosis of chondrocytes. Mechanically, LincRNA-Cox2 directly target to miR-150, acting as a ceRNA, and the effect of si-Cox2 on proliferation and apoptosis in chondrocytes was reversed by miR-150 inhibitor. Moreover, LincRNA-Cox2 had ability to activate wnt/β-catenin pathway to regulate chondrocytes proliferation and apoptosis.Conclusion: Silencing LincRNA-Cox2 plays a protective role in OA by enhancing the proliferation and suppressing apoptosis of chondrocytes, which was related with increase of miR-150 and activation of Wnt/β-catenin pathway.

show abstract

“…Apoptosis of the Leishmania-infected macrophage is delayed promoting survival and immune escape of the parasite [53][54][55]. Therefore, targeting the apoptosis-regulating miRNAs in Leishmania-infected macrophages may have potential to control the infection.…”

Section: Macrophage-related Micrornas Influence the Macrophages Apopt...mentioning

confidence: 99%

Bidirectional cytokine-microRNA control: A novel immunoregulatory framework in leishmaniasis

et al. 2022

View full text Add to dashboard Cite

As effector innate immune cells and as a host to the protozoan parasite Leishmania, macrophages play a dual role in antileishmanial immunoregulation. The 2 key players in this immunoregulation are the macrophage-expressed microRNAs (miRNAs) and the macrophage-secreted cytokines. miRNAs, as small noncoding RNAs, play vital roles in macrophage functions including cytokines and chemokines production. In the reverse direction, Leishmania-regulated cytokines alter miRNAs expression to regulate the antileishmanial functions of macrophages. The miRNA patterns vary with the time and stage of infection. The cytokine-regulated macrophage miRNAs not only help parasite elimination or persistence but also regulate cytokine production from macrophages. Based on these observations, we propose a novel immunoregulatory framework as a scientific rationale for antileishmanial therapy.

show abstract

The role of Bax in the apoptosis of Leishmania-infected macrophages

Cited by 19 publications

References 49 publications

Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

Role of lincRNA‑Cox2 targeting miR‑150 in regulating the viability of chondrocytes in osteoarthritis

LincRNA-Cox2 targeting miR-150 regulating the proliferation and apoptosis of chondrocytes in osteoarthritis

Bidirectional cytokine-microRNA control: A novel immunoregulatory framework in leishmaniasis

Contact Info

Product

Resources

About