The role of C/EBP homologous protein (CHOP) in regulating macrophage polarization in RAW264.7 cells

Peng, Shuang; Duan, Mengyun; Liu, Cuiliu; Li, Lingrui; Zhang, Xing; Ren, Boxu; Tian, Hongyang

doi:10.1111/1348-0421.12937

Cited by 7 publications

(5 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To prove this, the target gene or protein was usually interrupted by an inhibitor/agonist, or knockdown/over-expressing [50,51]. AS, a STAT6-specific inhibitor, inhibits IL-4induced STAT6 activation in vitro and in vivo, thereby attenuating macrophage M2 polarization [52,53] and number [54]. AS was demonstrated by a decrease in Arg1, Fizz1, Ym1, and CD206 [55], which can attenuate the progression of tumors in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization

Yang

Yan³

et al. 2023

Molecules

View full text Add to dashboard Cite

Pulsatilla saponins (PS) extracts from Pulsatilla chinensis (Bge.) Regel, are a commonly used traditional Chinese medicine. In the previous study, we found Pulsatilla saponins displayed anti-tumor activity without side effects such as bone marrow suppression. However, the mechanism of the anti-tumor effect was not illustrated well. Since M2-like tumor-associated macrophages (TAMs) that required activation of the signal transducer and activator of transcription 6 (STAT6) for polarization are the important immune cells in the tumor microenvironment and play a key role in tumor progress and metastasis, this study aimed to confirm whether Pulsatilla saponins could inhibit the development and metastasis of tumors by inhibiting the polarization of M2 macrophages. We investigated the relevance of M2 macrophage polarization and the anti-tumor effects of Pulsatilla saponins in vitro and in vivo. In vitro, Pulsatilla saponins could decrease the mRNA level of M2 marker genes Arg1, Fizz1, Ym1, and CD206, and the down-regulation effect of phosphorylated STAT6 induced by IL-4; moreover, the conditioned medium (CM) from bone marrow-derived macrophages (BMDM) treated with Pulsatilla saponins could inhibit the proliferation and migration of B16-F0 cells. In vivo, Pulsatilla saponins could reduce the number of lung metastasis loci, down-regulate the expression of M2 marker genes, and suppress the expression of phosphorylated STAT6 in tumor tissues. Furthermore, we used AS1517499 (AS), a STAT6 inhibitor, to verify the role of PS on M2 macrophage polarization both in vitro and in vivo. We found that Pulsatilla saponins failed to further inhibit STAT6 activation; the mRNA level of Arg1, Fizz1, Ym1, and CD206; and the proliferation and migration of B16-F0 cells after AS1517499 intervention in vitro. Similar results were obtained in vivo. These results illustrated that Pulsatilla saponins could effectively suppress tumor progress by inhibiting the polarization of M2 macrophages via the STAT6 signaling pathway; this revealed a novel mechanism for its anti-tumor activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization

Yang

Yan³

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…TNF receptor associated factor 6 (TRAF6) is an important signaling node in the Toll-like receptor pathway, which can initiate the transcription of inflammation-related target genes ( 40 ). However, members of the CCAAT enhancer binding protein family and signal transducer and activator of transcription (STAT) family have been identified as key mediators of these responses ( 41 , 42 ). In addition, the regulators of lipid metabolism peroxisome proliferator-activated receptor γ, circular RNAs, microRNAs, and long noncoding RNAs have been shown to be key regulators of macrophage polarization in both in vitro and in vivo models ( 43 – 46 ) ( Figure 1 ).…”

Section: Characteristics Of Macrophages and Their Pathophysiological ...mentioning

confidence: 99%

Role of macrophages in pulmonary arterial hypertension

et al. 2023

View full text Add to dashboard Cite

Pulmonary arterial hypertension (PAH) is a severe cardiopulmonary vascular disease characterized by progressive pulmonary artery pressure elevation, increased pulmonary vascular resistance and ultimately right heart failure. Studies have demonstrated the involvement of multiple immune cells in the development of PAH in patients with PAH and in experimental PAH. Among them, macrophages, as the predominant inflammatory cells infiltrating around PAH lesions, play a crucial role in exacerbating pulmonary vascular remodeling in PAH. Macrophages are generally polarized into (classic) M1 and (alternative) M2 phenotypes, they accelerate the process of PAH by secreting various chemokines and growth factors (CX3CR1, PDGF). In this review we summarize the mechanisms of immune cell action in PAH, as well as the key factors that regulate the polarization of macrophages in different directions and their functional changes after polarization. We also summarize the effects of different microenvironments on macrophages in PAH. The insight into the interactions between macrophages and other cells, chemokines and growth factors may provide important clues for the development of new, safe and effective immune-targeted therapies for PAH.

show abstract

“…Pretreatment with 10 mg/kg AS157499 1 h before ovalbumin challenge significantly suppressed hypercontractility of bronchial tissues, IL-13 production, and STAT6 phosphorylation [233]. More recently, several studies have evaluated AS157499 in the treatment of asthma and immune regulation [234][235][236][237]. In the context of cancer, Binnemars-Postma et al showed that STAT6 activation plays a critical role in M2 macrophage polarization [237].…”

Section: Stat6mentioning

confidence: 99%

The Role of STATs in Ovarian Cancer: Exploring Their Potential for Therapy

Standing

Feess

Kodiyalam

et al. 2023

Cancers

View full text Add to dashboard Cite

Ovarian cancer (OvCa) is a deadly gynecologic malignancy that presents many clinical challenges due to late-stage diagnoses and the development of acquired resistance to standard-of-care treatment protocols. There is an increasing body of evidence suggesting that STATs may play a critical role in OvCa progression, resistance, and disease recurrence, and thus we sought to compile a comprehensive review to summarize the current state of knowledge on the topic. We have examined peer reviewed literature to delineate the role of STATs in both cancer cells and cells within the tumor microenvironment. In addition to summarizing the current knowledge of STAT biology in OvCa, we have also examined the capacity of small molecule inhibitor development to target specific STATs and progress toward clinical applications. From our research, the best studied and targeted factors are STAT3 and STAT5, which has resulted in the development of several inhibitors that are under current evaluation in clinical trials. There remain gaps in understanding the role of STAT1, STAT2, STAT4, and STAT6, due to limited reports in the current literature; as such, further studies to establish their implications in OvCa are necessitated. Moreover, due to the deficiency in our understanding of these STATs, selective inhibitors also remain elusive, and therefore present opportunities for discovery.

show abstract

The role of C/EBP homologous protein (CHOP) in regulating macrophage polarization in RAW264.7 cells

Cited by 7 publications

References 48 publications

Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization

Pulsatilla Saponins Inhibit Experimental Lung Metastasis of Melanoma via Targeting STAT6-Mediated M2 Macrophages Polarization

Role of macrophages in pulmonary arterial hypertension

The Role of STATs in Ovarian Cancer: Exploring Their Potential for Therapy

Contact Info

Product

Resources

About