The Role of Calcium in the Penetration of Bacteriophage T5 Into Its Host

Luria, Salvador E.; Steiner, Doris L.

doi:10.1128/jb.67.6.635-639.1954

Cited by 71 publications

(24 citation statements)

References 8 publications

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“…These results are in line with earlier observations made by different scientific groups. Requirement for calcium has been reported for phage penetration of nucleic material into the host cytoplasm [7,14,17]. Potter and Nelson [18] confirmed that the lactic streptococcus bacteriophage system also requires calcium and related ions in the process of penetration.…”

Section: Phage Adsorption (Min)mentioning

confidence: 87%

“…Hence, the presence of calcium in the medium facilitated the primary attachment of phage MR-10 by decreasing the repulsion and by neutralizing the charges between the host cell surface polymers and the phage surface. Calcium has been reported to influence other events in the phage cycle as well [7,[14][15][16]. To elucidate this, one step growth curve of the phage was studied in the absence and presence of 5 mM CaCl 2 .…”

Section: Phage Adsorption (Min)mentioning

confidence: 99%

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Essential role of calcium in the infection process of broad‐spectrum methicillin‐resistant Staphylococcus aureus bacteriophage

Chhibber

Kaur

2013

J. Basic Microbiol.

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Phage therapy presents an alternative therapeutic option in treating infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains not responding to antibiotic therapy. However, it is essential to study the role of external factors that may influence the yield and potency of phage preparations intended for use in various in vitro and in vivo studies. The present study focuses on the effects of calcium in the entire infection process of a broad-spectrum lytic bacteriophage: MR-10. The presence of calcium increased the adsorption rate of the phage and also participated in the process of penetration of the phage genome into the host cytoplasm. A final concentration of 5 mM of calcium ions supplemented in soft agar during the phage titration process significantly increased the phage titer. Hence, incorporation of such divalent cations during the isolation of lytic phages active against MRSA strains and during the preparation of high-titer active phage preparations would definitely increase the isolation frequency and the final phage yield. This will contribute towards more effective phage preparations for use in treatments against MRSA infections.

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Section: Phage Adsorption (Min)mentioning

confidence: 87%

Section: Phage Adsorption (Min)mentioning

confidence: 99%

Essential role of calcium in the infection process of broad‐spectrum methicillin‐resistant Staphylococcus aureus bacteriophage

Chhibber

Kaur

2013

J. Basic Microbiol.

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“…Calcium also functions in certain transformations by pneumococcal DNA (18) and, as measured by blending experiments, in the entry of T5 DNA into E. coli (19). In the latter, magnesium can replace calcium, though less effectively.…”

Section: Discussionmentioning

confidence: 99%

Infection of Mycobacterium Smegmatis With D29 Phage Dna

Tokunaga

Sellers

1964

The Journal of Experimental Medicine

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Specific biological activity of deoxyribonucleic acid (DNA) was first estabfished by the experiments of Avery, MacLeod, and McCarty (1). These investigators showed that the substance responsible for the transformation of pneumococcal types was DNA. Current literature contains many examples of the biological activity of DNA including that isolated from bacteriophages. Spizizen (2) and Frazer et al. (3) infected protoplasts of Escherichia coli with subviral extracts from T, cofiphage. Guthrie and Sinsheimer (4) infected protoplasts of E. coli with purified DNA from $X174 phage. Kaiser and Hogness (5) were able to transform E. coli with DNA from kdg phage, providing that nontransducing helper phages were added to the bacteria along with the DNA. More recently Romig (6) infected a transformable strain of Bacillus subtilis with DNA from a phage which he had isolated from soil and designated as SP3.The experiments reported here extend these observations to include the mycobacteriophages. It was found that DNA from mycobacteriophage D29 could infect its host. Bacteria susceptible to infection with intact phage were also susceptible to phage DNA; no alteration in their physiologic state was required. Bu.~ersl--Tri*: Sigma Chemical Company, St. Louis. A 1 m stock solution adjusted to the desired pH between 7 and 9 by adding 10 N HC1 and used in the final concentration of 0.05 or 0.007 M. Materials and Methods

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“…30 times) when the temperature rose from 4°C to 40°C when λ phage was incubated with E. coli strain Ymel. The role of Ca 2+ and Mg 2 + ions in phage‐host interaction may be in the adsorption, penetration processes or in other growth stages of phage (d’Herelle 1926;Luria and Steiner 1954; Adams 1959; Moldovan et al. 2007).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of bacteriophages specific to the catfish pathogen,Edwardsiella ictaluri

Walakira

Carrias

Hossain

et al. 2008

Journal of Applied Microbiology

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Aims: To identify and characterize bacteriophages specific for Edwardsiella ictaluri, the causative agent for enteric septicemia of catfish (ESC). Methods and Results: Two bacteriophages were isolated that infect Edw. ictaluri. They both produce clear plaques, have icosahedral heads with a non‐rigid tail, and are tentatively classified as Siphoviridae. Phages ΦeiDWF and ΦeiAU are dsDNA viruses with approximate genome sizes of 40 and 45 kb, respectively. The addition of 500 μmol l−1 CaCl2 enhanced phage titres. Both phages have a latent period of 40 min and an estimated burst size of 270. Every Edw. ictaluri strain tested was susceptible to phage infection with variable plaquing efficiencies and with no evidence of lysogeny, with no plaques detected on other bacterial species. Conclusions: Two unique bacteriophages were isolated that show host‐specificity for Edw. ictaluri, have temperature and metal cation‐dependent infectivity, and are tentatively placed within the family Siphoviridae. Significance and Impact of the Study: This is the first report of bacteriophages specific to Edw. ictaluri, an important fish pathogen affecting farm‐raised channel catfish. Initial characterization of these bacteriophages has demonstrated their potential use as biotherapeutic and diagnostic agents associated with ESC.

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The Role of Calcium in the Penetration of Bacteriophage T5 Into Its Host

Cited by 71 publications

References 8 publications

Essential role of calcium in the infection process of broad‐spectrum methicillin‐resistant Staphylococcus aureus bacteriophage

Essential role of calcium in the infection process of broad‐spectrum methicillin‐resistant Staphylococcus aureus bacteriophage

Infection of Mycobacterium Smegmatis With D29 Phage Dna

Identification and characterization of bacteriophages specific to the catfish pathogen,Edwardsiella ictaluri

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