The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass

Zverlov, Vladimir V.; Köck, Daniela; Schwarz, Wolfgang H.

doi:10.1007/978-3-662-45209-7_12

Cited by 8 publications

(8 citation statements)

References 116 publications

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“…Addressing this issue, Maus et al [ 11 ] isolated and genetically characterized novel cellulolytic, hydrolytic, and acidogenic/acetogenic Bacteria as well as methanogenic Archaea originating from different anaerobic digestion communities. This study and earlier work [ 9 , 11 ] showed that (hemi)cellulolytic Bacteria mostly represented a minority within the entire biogas microbiome despite their unquestionably pivotal role in initial biomass breakdown, which can be crucial and rate-limiting for the entire biogas process [ 4 , 12 ]. Therefore, it is postulated that few true cellulolytic Bacteria cooperate intimately and synergistically along with saccharolytic and synthrophic Bacteria [ 13 , 14 , 15 ].…”

Section: Introductionsupporting

confidence: 61%

“…The microbial conversion of lignocellulosic biomass to methane-rich biogas has become an important sustainable generating industry [ 1 , 2 , 3 ]. The process can be divided into several steps with different bacterial or archaeal organisms actively involved: (i) hydrolysis of the insoluble raw substrate by hydrolytic, saccharolytic, and peptolytic Bacteria; (ii) acidogenesis and acetogenesis by acidogenic and/or acetogenic Bacteria, including syntrophic oxidizers of acetate or other intermediates; and (iii) methanogenesis by methanogenic Archaea [ 4 ] with a diverse spectrum metabolic pathways for methanogenesis, mainly aceticlastic, hydrogenotrophic, or H 2 and methylotrophic methanogenesis [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

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Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum

et al. 2020

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Bacterial hydrolysis of polysaccharides is an important step for the production of sustainable energy, for example during the conversion of plant biomass to methane-rich biogas. Previously, Hungateiclostridium thermocellum was identified as cellulolytic key player in thermophilic biogas microbiomes with a great frequency as an accompanying organism. The aim of this study was to physiologically characterize a recently isolated co-culture of H. thermocellum and the saccharolytic bacterium Defluviitalea raffinosedens from a laboratory-scale biogas fermenter. The characterization focused on cellulose breakdown by applying the measurement of cellulose hydrolysis, production of metabolites, and the activity of secreted enzymes. Substrate degradation and the production of volatile metabolites was considerably enhanced when both organisms acted synergistically. The metabolic properties of H. thermocellum have been studied well in the past. To predict the role of D. raffinosedens in this bacterial duet, the genome of D. raffinosedens was sequenced for the first time. Concomitantly, to deduce the prevalence of D. raffinosedens in anaerobic digestion, taxonomic composition and transcriptional activity of different biogas microbiomes were analyzed in detail. Defluviitalea was abundant and metabolically active in reactor operating at highly efficient process conditions, supporting the importance of this organism for the hydrolysis of the raw substrate.

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Section: Introductionsupporting

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum

et al. 2020

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“…Biogas plants harbor complex microbial communities that are essential for the different steps of biogas production. However, the overall biogas production rates are limited and depend on the initial hydrolysis of the plant biomass [ 2 , 8 , 58 ]. To identify possible limitations regarding the overall hydrolytic performance of biogas plants, we sampled and analyzed a typical one-stage agricultural plant with respect to its taxonomic structure and its metagenome content.…”

Section: Resultsmentioning

confidence: 99%

“…Published research implies that the diversity ranges from several hundred to several thousand microbial species in active biogas reactors [ 3 , 4 , 6 , 7 ]. Interestingly, it was further reported that the overall production of biogas is probably limited due to the relatively slow hydrolysis of the agricultural plant biomass [ 8 ]. Thereby, Clostridia appear to play a major role during the initial biomass degradation.…”

Section: Introductionmentioning

confidence: 99%

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Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies

Güllert

Fischer

Turaev

et al. 2016

Biotechnol Biofuels

129

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BackgroundThe diverse microbial communities in agricultural biogas fermenters are assumed to be well adapted for the anaerobic transformation of plant biomass to methane. Compared to natural systems, biogas reactors are limited in their hydrolytic potential. The reasons for this are not understood.ResultsIn this paper, we show that a typical industrial biogas reactor fed with maize silage, cow manure, and chicken manure has relatively lower hydrolysis rates compared to feces samples from herbivores. We provide evidence that on average, 2.5 genes encoding cellulolytic GHs/Mbp were identified in the biogas fermenter compared to 3.8 in the elephant feces and 3.2 in the cow rumen data sets. The ratio of genes coding for cellulolytic GH enzymes affiliated with the Firmicutes versus the Bacteroidetes was 2.8:1 in the biogas fermenter compared to 1:1 in the elephant feces and 1.4:1 in the cow rumen sample. Furthermore, RNA-Seq data indicated that highly transcribed cellulases in the biogas fermenter were four times more often affiliated with the Firmicutes compared to the Bacteroidetes, while an equal distribution of these enzymes was observed in the elephant feces sample.ConclusionsOur data indicate that a relatively lower abundance of bacteria affiliated with the phylum of Bacteroidetes and, to some extent, Fibrobacteres is associated with a decreased richness of predicted lignocellulolytic enzymes in biogas fermenters. This difference can be attributed to a partial lack of genes coding for cellulolytic GH enzymes derived from bacteria which are affiliated with the Fibrobacteres and, especially, the Bacteroidetes. The partial deficiency of these genes implies a potentially important limitation in the biogas fermenter with regard to the initial hydrolysis of biomass. Based on these findings, we speculate that increasing the members of Bacteroidetes and Fibrobacteres in biogas fermenters will most likely result in an increased hydrolytic performance.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-016-0534-x) contains supplementary material, which is available to authorized users.

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Efficient Utilization and Bioprocessing of Agro-Industrial Waste

Kumar

Kumari

Dindhoria

et al. 2021

Sustainable Agriculture Reviews 56

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The Role of Cellulose-Hydrolyzing Bacteria in the Production of Biogas from Plant Biomass

Cited by 8 publications

References 116 publications

Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum

Importance of Defluviitalea raffinosedens for Hydrolytic Biomass Degradation in Co-Culture with Hungateiclostridium thermocellum

Deep metagenome and metatranscriptome analyses of microbial communities affiliated with an industrial biogas fermenter, a cow rumen, and elephant feces reveal major differences in carbohydrate hydrolysis strategies

Efficient Utilization and Bioprocessing of Agro-Industrial Waste

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