The role of cholesterol in membrane activity of digitonin: Experimental and theoretical model studies

Korchowiec, Beata; Janikowska-Sagan, Maria; Kwiecińska, Klaudia; Stachowicz‐Kuśnierz, Anna; Korchowiec, Jacek

doi:10.1016/j.molliq.2020.114598

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…The final composition of ternary SM/POPC/CHOL monolayers was 45/45/10, 35/35/30, and 25/25/50. Some saponins show a high affinity for cholesterol, which is often linked to their hemolytic activity [ 27 , 28 , 29 ]. However, it was recently shown that membrane activity of the ginsenoside Rh2 saponin was boosted by the interaction with sphingomyelin but was worsened by cholesterol [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes

Korchowiec

Kwiecińska

et al. 2022

IJMS

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In view of the possible medical applications of saponins, the molecular structure of a GOTCAB saponin from the roots of Gypsophila paniculata L. was determined by NMR. The biological activity of saponins may depend on the interaction with cell membranes. To obtain more insight in the mechanism of membrane-related saponin function, an experimental and theoretical study was conducted. Ternary lipid systems composed of sphingomyelin, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, and cholesterol were used as models of mammalian cell membranes. The membrane–saponin interaction was studied experimentally by monitoring surface pressure in the monomolecular films formed at the air–aqueous subphase interface. The behavior of GOTCAB saponin in a water box and model monolayer systems was characterized by molecular dynamics simulations. The results obtained showed that, in the systems used, cholesterol had a decisive effect on the interaction between GOTCAB and phosphocholine or sphingomyelin as well as on its location within the lipid film.

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Section: Resultsmentioning

confidence: 99%

The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes

Korchowiec

Kwiecińska

et al. 2022

IJMS

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“…Saponins are known for their high affinity to membrane lipids, especially to sterols [14,16,22,23,25,29,55,56]. Consequently, numerous postulated mechanisms of membranolytic activity of saponins assume that the primary site of saponin attack is cholesterol [57][58][59][60][61].…”

Section: Resultsmentioning

confidence: 99%

“…Thanks to their surface activity and taste-modifying properties (sweet or bitter taste), often combined with high biological activity, saponins find numerous applications in the food, cosmetic, and pharmaceutical industries. For pharmaceutical applications, the saponin-cholesterol interaction, described for the first time more than a century ago [17,18], is especially important [19][20][21][22][23][24][25][26][27]. For many years, precipitation of cholesterol by a steroid saponin, digitonin, was even a basis of quantitative analysis of cholesterol in blood [28].…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity of Quillaja saponaria Saponins towards Lung Cells Is Higher for Cholesterol-Rich Cells

et al. 2021

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The purpose of the study was to compare cytotoxicity of two Quillaja saponaria bark saponin (QBS) mixtures against two lung cell lines: normal MRC-5 fibroblast cell line and tumor A-549 epithelial cells of lungs’ alveoli. The study, performed both at a macro-scale and in a dedicated microfluidic device, showed that QBS was more toxic to the cell line more abundant in cholesterol (MRC-5). The QBS mixture with higher saponin fraction was found to be more cytotoxic towards both cell lines. The results may help to better understand the cytotoxicity of saponin-rich herbal medicines towards normal and tumor cells depending on their cholesterol content.

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“…For this reason, complementary methods based on model cell membranes, which allow to investigate interactions between nanomaterials and phospholipids at the molecular level, have attracted much attention . A Langmuir technique is one of the well-known and widely applied methods for the preparation of model cell membranes. − This technique has also been successfully used in studies of interactions between different nanoparticles and phospholipids, including MNPs. − A single Langmuir monolayer formed from phospholipids can be considered as an oversimplified experimental model of a cell membrane consisting of two weakly coupled monolayers, which is a complex and dynamic system. The composition of a model membrane can be precisely designed with the Langmuir method, which allows the formation of one- or multi-component monolayers from different types of phospholipids and other components of membranes.…”

Section: Introductionmentioning

confidence: 99%

Influence of the Type of Biocompatible Polymer in the Shell of Magnetite Nanoparticles on Their Interaction with DPPC in Two-Component Langmuir Monolayers

Piosik,

Modlińska,

Gołaszewski

et al. 2024

J. Phys. Chem. B

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Magnetite nanoparticles (MNPs) are attractive nanomaterials for applications in magnetic resonance imaging, targeted drug delivery, and anticancer therapy due to their unique properties such as nontoxicity, wide chemical affinity, and intrinsic superparamagnetism. Their functionalization with polymers such as chitosan or poly(vinyl alcohol) (PVA) can not only improve their biocompatibility and biodegradability but it also plays an important role in their interactions with biological cells. In this work, the effect of the functionalization of MNPs with chitosan, PVA, and their blend on model cell membranes formed from 1,2-dipalmitoylsn-glycerol-3-phosphocholine (DPPC) using a Langmuir technique was studied. The studies performed showed that the type of biocompatible polymer in the MNP shell plays a crucial role in the effectiveness of its adsorption process into the model cell membrane. Modification of MNPs with chitosan facilitates significantly more effective adsorption than coating them with PVA or with a chitosan and PVA blend. The presence of all the investigated MNPs in the DPPC monolayer at low concentrations does not affect its thermodynamic state, fluidity, or morphology, which is promising in terms of their biocompatibility. On the other hand, their high concentration (molar fraction above ≈0.05) exerts a disruptive effect on the model cell membrane and results in their aggregation, leading probably to the loss of their superparamagnetic properties essential for nanomedicine.

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The role of cholesterol in membrane activity of digitonin: Experimental and theoretical model studies

Cited by 8 publications

References 29 publications

The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes

The Molecular Bases of the Interaction between a Saponin from the Roots of Gypsophila paniculata L. and Model Lipid Membranes

Cytotoxicity of Quillaja saponaria Saponins towards Lung Cells Is Higher for Cholesterol-Rich Cells

Influence of the Type of Biocompatible Polymer in the Shell of Magnetite Nanoparticles on Their Interaction with DPPC in Two-Component Langmuir Monolayers

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