The role of circular RNAs in viral infection and related diseases

Xie, He; Sun, Honggang; Mu, Rongrong; Li, Shilin; Li, Yujia; Yang, Chunhui; Xu, Min; Duan, Xiaoqiong; Chen, Limin

doi:10.1016/j.virusres.2020.198205

Cited by 33 publications

(24 citation statements)

References 90 publications

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“…circRNAs were originally thought to be by-products of aberrant splicing with little functional potential [10]. Novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have enabled comprehensive studies of circRNAs to be performed [9], and several studies have addressed the identi cation, characterization, and function of circRNAs during viral infection [32]. For example, human cytomegalovirus (HCMV) could cause signi cant changes in host circRNAs and it was found that circSP100 potentially bound to 10 HCMVencoding proteins, which further in uenced the process of HCMV infection [8].…”

Section: Discussionmentioning

confidence: 99%

CircRNA Expression Patterns and circRNA-miRNA-mRNA Networks During CV-A16 Infection of SH-SY5Y Cells

Yang

Zhao

et al. 2021

Preprint

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Coxsackievirus A16 (CV-A16) has caused worldwide epidemics of hand, foot, and mouth disease (HFMD), particularly among infants and preschool children. Circular RNAs (circRNAs) are a recently identified class of noncoding RNA molecules that participate in the occurrence and development of numerous infectious diseases. To date, the function of circRNAs has been a heavily researched topic, and their role in CV-A16 infection has not been fully elucidated. In this study, the viral effects of CV-A16 on the cellular circRNA transcriptome were investigated using next-generation sequencing technology. In this study, a total of 8726, 8611 and 6826 circRNAs were identified in the control, CV-A16-12 h and CV-A16-24 h groups, respectively. Further analysis showed that 1769 and 1192 circRNAs were differentially expressed in the CV-A16-12 h and CV-A16-24 h groups, respectively. Moreover, the common differentially expressed circRNAs were screened out and used for functional annotation analysis, which demonstrated that the parent genes of differentially expressed circRNAs might be associated with the viral infection process, especially the “Immune system process” in GO analysis and the “Inflammation mediated by chemokine and cytokine signaling pathway” in KEGG analysis. Additionally, 6 dysregulated circRNAs were selected for validation and were verified to be consistent with the sequencing results. Additionally, we further constructed a circRNA-miRNA-mRNA regulatory network that suggested the putative function of circRNAs as cellular miRNA decoys to indirectly regulate gene expression during CV-A16 infection, especially the hsa_circ_0004447/hsa-miR-942-5p/MMP2, hsa_circ_0078617/hsa-miR-6780b-5p/MMP2 and hsa_circ_0078617/hsa-miR-5196-5p/MMP2 regulatory axes. Considering all of these results, to the best of our knowledge, this study is the first to present a comprehensive overview of circRNAs induced by CV-A16, and this research demonstrated that a network of enriched circRNAs and circRNA-associated competitive endogenous RNAs (ceRNAs) is involved in the regulation of CV-A16 infection, thereby helping to elucidate the mechanisms underlying CV-A16-host interactions.

show abstract

Section: Discussionmentioning

confidence: 99%

CircRNA Expression Patterns and circRNA-miRNA-mRNA Networks During CV-A16 Infection of SH-SY5Y Cells

Yang

Zhao

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…circRNAs were originally thought to be by-products of aberrant splicing with little functional potential [ 10 ]. Novel bioinformatic approaches coupled with biochemical enrichment strategies and deep sequencing have enabled comprehensive studies of circRNAs to be performed [ 9 ], and several studies have addressed the identification, characterization, and function of circRNAs during viral infection [ 33 ]. For example, human cytomegalovirus (HCMV) infection causes significant changes in host circRNA expression, and it was found that circSP100 potentially bound to 10 HCMV-encoding proteins, which further influenced the process of HCMV infection [ 8 ].…”

Section: Discussionmentioning

confidence: 99%

circRNA expression patterns and circRNA-miRNA-mRNA networks during CV-A16 infection of SH-SY5Y cells

Yang

Zhao

et al. 2021

Arch Virol

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Coxsackievirus A16 (CV-A16) has caused worldwide epidemics of hand, foot, and mouth disease (HFMD) in infants and preschool children. Circular RNAs (circRNAs), a class of noncoding RNA molecules, participate in the progression of viral infectious diseases. Although the function of circRNAs has been a heavily researched topic, their role in CV-A16 infection is still unclear. In this study, the viral effects of CV-A16 on the cellular circRNA transcriptome were investigated using next-generation sequencing technology. The results showed that a total of 8726, 8611, and 6826 circRNAs were identified at 0, 12, and 24 h postinfection, respectively. Moreover, it was found that 1769 and 1192 circRNAs were differentially expressed in at 12 and 24 h postinfection, respectively. The common differentially expressed circRNAs were used for functional annotation analysis, and it was found that the parent genes of differentially expressed circRNAs might be associated with the viral infection process, especially the “Immune system process” in GO analysis and the “Inflammation mediated by chemokine and cytokine signaling pathway” in KEGG analysis. Subsequently, circRNA-miRNA-mRNA regulatory networks were constructed, and the hsa_circ_0004447/hsa-miR-942-5p/MMP2, hsa_circ_0078617/hsa-miR-6780b-5p/MMP2 and hsa_circ_0078617/hsa-miR-5196-5p/MMP2 regulatory axes were identified by enrichment analysis as important networks during the progression of CV-A16 infection. Finally, six dysregulated circRNAs were selected for validation and were verified to be consistent with the sequencing results. Considering all of these results, to the best of our knowledge, this study is the first to present a comprehensive overview of circRNAs induced by CV-A16 infection, and this research demonstrated that a network of enriched circRNAs and circRNA-associated competitive endogenous RNAs (ceRNAs) is involved in the regulation of CV-A16 infection, thereby helping to elucidate the mechanisms underlying CV-A16-host interactions. Supplementary Information The online version contains supplementary material available at 10.1007/s00705-021-05190-z.

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“…miR-27b-3p in DF-1 cells increases the expression of chicken IFN-β, IRF3, and NF-κB by directly binding to SOCS3/6, preventing IBDV replication in host cells. Suppression of endogenous miR-27b-3p through its inhibitors suppresses IFN-β, IRF3, and NF-κB, leading to increased expression of SOCS3/6 and increased replication of IBDV [ 34 ].…”

Section: The Role Of Mirnas In Viral Infectionmentioning

confidence: 99%

The potential use of microRNAs as a therapeutic strategy for SARS-CoV-2 infection

Stojanović²,

Yasamineh

et al. 2021

Arch Virol

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Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). To date, there is no effective therapeutic approach for treating SARS-CoV-2 infections. MicroRNAs (miRNAs) have been recognized to target the viral genome directly or indirectly, thereby inhibiting viral replication. Several studies have demonstrated that host miRNAs target different sites in SARS-CoV-2 RNA and constrain the production of essential viral proteins. Furthermore, miRNAs have lower toxicity, are more immunogenic, and are more diverse than protein-based and even plasmid-DNA-based therapeutic agents. In this review, we emphasize the role of miRNAs in viral infection and their potential use as therapeutic agents against COVID-19 disease. The potential of novel miRNA delivery strategies, especially EDV™ nanocells, for targeting lung tissue for treatment of SARS-CoV-2 infection is also discussed.

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The role of circular RNAs in viral infection and related diseases

Abstract: Highlights CircRNAs are formed by pre-mRNA through “back-splicing”. CircRNAs regulate host immune response and virus replication. CircRNAs have potential as diagnostic markers or treatment targets for viral infection.

Cited by 33 publications

References 90 publications

CircRNA Expression Patterns and circRNA-miRNA-mRNA Networks During CV-A16 Infection of SH-SY5Y Cells

CircRNA Expression Patterns and circRNA-miRNA-mRNA Networks During CV-A16 Infection of SH-SY5Y Cells

circRNA expression patterns and circRNA-miRNA-mRNA networks during CV-A16 infection of SH-SY5Y cells

The potential use of microRNAs as a therapeutic strategy for SARS-CoV-2 infection

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