The role of Cl− ions in the neurotransmitter and redox regulation of ion movements through isolated frog skin

Puppi, A; Dely, M.

doi:10.1016/0306-4492(78)90050-3

Cited by 2 publications

(1 citation statement)

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“…Puppi and Dely (1978) claimed chloride was required for the full effect of high concentrations (250 gM) of acetylcholine on the skin. Throughout this work our concentrations of cholinoceptor agonists have been modest by comparison, and no effects of chloride removal were found.…”

Section: Discussionmentioning

confidence: 99%

Mechanisms for the effects of acetylcholine on sodium transport in frog skin

Cuthbert

Wilson

1981

J. Membrain Biol.

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In frog skin (Rana temporaria) acetylcholine applied to the serosal surface produces either a sustained inhibiton or sustained stimulation of short-circuit current (SCC). The former effect is accompanied by a reduction and the latter by an increase in total tissue conductance. Both effects of acetylcholine can be accounted for, within experimental error, by changes in net sodium flux across the tissue. By use of selective agonists and antagonists it is concluded that acetylcholine interacts with muscarinic receptors in the serosal membrane. The effects of cholinoceptor agents are also seen with isolated epithelium. The stimulatory effect of acetylcholine is potentiated by theophylline and blocked by inhibitors of prostaglandin synthetase and by mepacrine. It is suggested that acetylcholine stimulates transport by liberating prostaglandins which may then activate adenylcyclase. The inhibitory effect of acetylcholine is correlated with a reduction in cyclic AMP content of the epithelium. Calcium appears to be an important determinant of the type of response seen eith acetylcholine, but the mechanism is not known.

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Section: Discussionmentioning

confidence: 99%