With the progress of society and the rapid development of science and technology in the world, the 21st century will be the multimedia era. Therefore, physical education teaching in colleges and universities should take the initiative to seize the opportunity, adapt to social needs, change traditional teaching concepts, introduce modern teaching methods into physical education teaching, enrich physical education teaching content, stimulate students’ interest in sports knowledge and sports technology learning, broaden knowledge horizons, improve teaching quality, and promote the development of physical education. The traditional teaching mode restricts the development of physical education (PE) in many aspects, and one of the important contents of modern teaching methods is to master and use modern teaching equipment and means. Therefore, it is necessary to timely change the educational thought, update the educational concept, and actively explore and research new teaching methods that are suitable for the educational concept of the times, to ensure the adaptability, scientificity, and creativity of teaching methods. Based on the above background, the research content of this paper is the application of multimedia CAI technology in PE teaching. Using the methods of document interviews, analysis and induction, and online retrieval, on the basis of reading a large number of professional theoretical books on physical education, multimedia technology, and modern teaching and reform, the application of multimedia technology in physical education teaching in colleges and universities is analyzed. The application is researched at the theoretical level. This paper takes PE college 2019 students as the simulation object. The results show that the pass rate of professional English after the experiment is 15.33% higher than that before the experiment.