The role of Concanavalin A-binding fraction in cholesterol crystallization in native human bile

Carcinoembryonic antigen-related cell adhesion molecule 1 is the 85-kilodalton pronase-resistant biliary glycoprotein in the cholesterol crystallization promoting low density protein-lipid complex

“…23,35,36 Recently, Wang et al 37 could not confirm this activity when CABF was reconstituted with lipids extracted from human biles. Although this apparent contradiction may be because of differences in results with proteinfree lipids versus native biles, the results of Keulemans et al 38 suggest an alternative explanation: extraction of CABF from native biles almost abrogated crystallization in rapid nucleating bile but had very little effect in more slowly nucleating samples showing that only a subgroup of biles contains high activity in CABF. Possibly, Wang et al 37 isolated CABF from biles with a low CABF activity.…”

Increased Activity in the Biliary Con A-Binding Fraction Accounts for the Difference in Crystallization Behavior in Bile From Chilean Gallstone Patients Compared With Dutch Gallstone Patients

“…20 To avoid the complication of interacting factors in the present study, we have chosen to extract the complete CABF. The principle of the method has been published before, 14 raised whether activity of CABF may be artifactuous, 21 we have performed a series of additional control experiments. A disadvantage of the method is the dilution induced by the aqueous phase present in and adhering to the beads.…”

“…Human gallbladder bile from gallstone and nongallstone patients was incubated with Con A-sepharose beads (CA), using a slightly modified version of the method described by Keulemans et al 14 Three mL of bile was added to a graduated glass vial containing 0.5 mL packed Con A-sepharose beads, and 3 mL to a vial containing 0.5 mL packed sepharose (SEP) beads, as a control. The vials were incubated for 2 hours at room temperature under gentle rotation.…”

“…8,14 Biliary immunoglobulins, haptoglobin, and ␣-1-acidglycoprotein were quantified by ELISA both in Amsterdam and Santiago exactly as described previously. 8,14 Lipid Analysis. Cholesterol, bile salts, and phospholipids were measured by using standard techniques in both centers as described previously.…”

“…In their hands reconstitution of the concanavalin A-binding fraction (CABF) with lipids isolated from human bile did not change crystallization kinetics in these reconstituted systems. Keulemans et al 14 used a different (less invasive) approach to assess the importance of CABF. They incubated fresh native biles with Con A coupled to sepharose beads and after incubation determined crystallization kinetics in the CABF extracted fraction.…”