The Role of Connective Tissue Growth Factor (CTGF) in Fibroproliferative Processes and Tissues Fibrosis

Gojniczek, Katarzyna; Jurzak, Magdalena; Garncarczyk, Agnieszka

doi:10.2478/v10052-008-0004-0

Cited by 4 publications

(5 citation statements)

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“…34,38,39 The unregulated production of fibrotic tissue ultimately occludes deep connective tissues, muscles, and obliterates the microvasculature. 31–33,39,40 Importantly, this generalized understanding suggests that if local inflammation and fibroblast activity can be modulated enough to prevent the switch from normal to abnormal wound healing, then fibrosis and the associated dysphagia may also be mitigated.…”

Section: Discussionmentioning

confidence: 99%

Application of Manual Therapy for Dysphagia in Head and Neck Cancer Patients: A Preliminary National Survey of Treatment Trends and Adverse Events

Krisciunas

Vakharia

Lazarus

et al. 2019

Glob Adv Health Med

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Background Radiation-associated dysphagia is a common and debilitating consequence of treatment for head and neck cancer (HNC). Since commonly employed dysphagia therapy programs for HNC patients still lack authoritative efficacy, some speech-language pathologists (SLPs) have started employing manual therapy (MT) techniques in an attempt to prevent or rehabilitate dysphagia in this patient population. However, exceptionally little is known about the use of MT in this patient population. Objectives The purpose of this study was to describe practice patterns as well as the rate, type, and severity of adverse events associated with SLP provision of MT to HNC patients. Methods An Internet-based questionnaire geared toward SLPs who practice MT was developed and sent to SLPs practicing in the United States, 3 times, through 3 national listservs (American Speech Language Hearing Association [ASHA] Special Interest Division 13, ASHA Special Interest Division 3, and University of Iowa Voiceserv), over the course of 4 weeks. Results Of the 255 respondents, 116 (45.5%) performed MT on HNC patients. Of these 116 SLPs, 27.6% provided proactive MT during radiation, 62.1% provided 1 to 2 sessions per week, and 94.8% prescribed a MT home program. The rate, type, and severity of reported adverse events were similar between HNC and non-HNC patients. Conclusion This preliminary survey demonstrated that SLPs provide MT to HNC patients during and after cancer treatment, and that reported adverse events paralleled those experienced by noncancer patients. However, these results should be taken with caution, and a well-designed prospective study is needed to formally establish the safety and the preliminary efficacy of this novel clinical intervention.

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Section: Discussionmentioning

confidence: 99%

Application of Manual Therapy for Dysphagia in Head and Neck Cancer Patients: A Preliminary National Survey of Treatment Trends and Adverse Events

Krisciunas

Vakharia

Lazarus

et al. 2019

Glob Adv Health Med

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“…It was previously reported that ETAR antagonist significantly blocked ET-1induced increase in FN and COL-I in skin fibroblasts [30]. Other studies concluded that ET-1 also promoted the expression of ECM through ETAR in tissues such as myocardium, lung, kidney, and nipple [31][32][33].…”

Section: Blocking the Et-1+etar Pathway Prohibits Et-1 -Induced Incre...mentioning

confidence: 93%

The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP

et al. 2023

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Purpose: Excessive accumulation of extracellular matrix (ECM) in the trabecular meshwork (TM) increases resistance to the outflow of aqueous humor, which inevitably contributes to the elevation of intraocular pressure (IOP) in primary openangle glaucoma (POAG). Endothelin 1 (ET-1), a potent vasoconstrictor peptide, is reported to enhance profibrotic processes in multiple organs like liver, lung, and kidney tissues, which also significantly increases aqueous humor in POAG patients.Here we tested the hypothesis that ET-1 plays a key role in excessive accumulation in the trabecular meshwork and increase in IOP. Methods:To test the effect of ET-1 on expression of fibronectin (FN), collagen type IV (COL-IV) and its receptor pathway, cultured human TM cells (HTMCs) were treated with ET-1, ET-1+ ETAR antagonist (BQ123) or ETBR antagonist (BQ788). The protein expression levels of FN, and COL-IV were evaluated by western-blot. A time course effect of ET-1 on the transcription level of the connective tissue growth factor (CTGF) was investigated by qRT-PCR. Next, we down regulated the transcription level of CTGF by its antisense oligodeoxynucleotide sequence and then tested the expression levels of FN, and COL-IV. Lastly, we observed the effect of ET-1 on IOP changes, expression of FN, and COL-IV in an ex-vivo model of cultured anterior eye segment. Results:In cultured HTMs, the expression of FN and COL-IV was significantly increased after ET-1 treatment, and this could be blocked by ETAR antagonist (BQ123) but not ETBR antagonist (BQ788). Further, the CTGF mRNA level also increased significantly and reached the top level at 48h after ET-1 treatment. However, the induced ET-1 increased the expression of FN and COL-IV in HTMCs and could be reversed by the downregulation of CTGF. In an ex-vivo model, incensement of FN and COL-IV expression were observed too after ET-1 perfusion. Furthermore, IOP significantly increased after ET-1 administration. Nevertheless, this could be decreased by the ETAR antagonist (BQ123) but not the ETBR antagonist (BQ788). Conclusions:These results indicate that ET-1 in aqueous humor could lead to the abnormal synthesis of FN and COL-IV via the ETA-CTGF pathway which could result in the accumulation of ECM in trabecular meshwork and incensement of IOP.

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“…Finally, the Smad2/3/4 complex is transported to the nucleus to regulate transcription, upregulate P300 transcriptional activity, and promote histone H4K16 acetylation and collagen transcription. Moreover, TGF-β induces type I collagen production by stimulating the expression of the connective tissue growth factor (CTGF) gene [ 61 , 62 , 63 ]. Duncan et al, who conducted in vitro and in vivo studies using normal rat kidney fibroblasts, demonstrated that CTGF could induce collagen synthesis and that transfection with the antisense CTGF gene blocked TGF-β-mediated stimulation of collagen synthesis.…”

Section: Possible Mechanisms Of Oxidative Stress-induced Skin Cellula...mentioning

confidence: 99%

Possible Mechanisms of Oxidative Stress-Induced Skin Cellular Senescence, Inflammation, and Cancer and the Therapeutic Potential of Plant Polyphenols

Liu

Cheng

Xun

et al. 2023

IJMS

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As the greatest defense organ of the body, the skin is exposed to endogenous and external stressors that produce reactive oxygen species (ROS). When the antioxidant system of the body fails to eliminate ROS, oxidative stress is initiated, which results in skin cellular senescence, inflammation, and cancer. Two main possible mechanisms underlie oxidative stress-induced skin cellular senescence, inflammation, and cancer. One mechanism is that ROS directly degrade biological macromolecules, including proteins, DNA, and lipids, that are essential for cell metabolism, survival, and genetics. Another one is that ROS mediate signaling pathways, such as MAPK, JAK/STAT, PI3K/AKT/mTOR, NF-κB, Nrf2, and SIRT1/FOXO, affecting cytokine release and enzyme expression. As natural antioxidants, plant polyphenols are safe and exhibit a therapeutic potential. We here discuss in detail the therapeutic potential of selected polyphenolic compounds and outline relevant molecular targets. Polyphenols selected here for study according to their structural classification include curcumin, catechins, resveratrol, quercetin, ellagic acid, and procyanidins. Finally, the latest delivery of plant polyphenols to the skin (taking curcumin as an example) and the current status of clinical research are summarized, providing a theoretical foundation for future clinical research and the generation of new pharmaceuticals and cosmetics.

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The Role of Connective Tissue Growth Factor (CTGF) in Fibroproliferative Processes and Tissues Fibrosis

Cited by 4 publications

References 71 publications

Application of Manual Therapy for Dysphagia in Head and Neck Cancer Patients: A Preliminary National Survey of Treatment Trends and Adverse Events

Application of Manual Therapy for Dysphagia in Head and Neck Cancer Patients: A Preliminary National Survey of Treatment Trends and Adverse Events

The effect of ET1-CTGF mediated pathway on the accumulation of extracellular matrix in the trabecular meshwork and its contribution to the increase in IOP

Possible Mechanisms of Oxidative Stress-Induced Skin Cellular Senescence, Inflammation, and Cancer and the Therapeutic Potential of Plant Polyphenols

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