The Role of Cortical Geometry in the Nuclear Development of <i>Tetrahymena thermophila</i>

Gaertig, Jacek; Cole, Elliot

doi:10.1111/j.1550-7408.2000.tb00095.x

Cited by 10 publications

(8 citation statements)

References 14 publications

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“…In the elo1-1 cells, the positions of both the dividing micronucleus and macronucleus were excessively posterior, but in a local agreement with the division plane position (Figure 2, E and F). In ciliates the positions of nuclei in the endoplasm are guided by cues from the cell cortex (Weisz 1951;de Terra 1973de Terra , 1975Cohen and Beisson 1980;Gaertig and Cole 2000). Thus, the cortical guidance of nuclei is unaffected by elo1-1.…”

Section: Resultsmentioning

confidence: 99%

“…In the Tetrahymena con1-1 mutant, an altered shape of the posterior cell end correlates with a posteriorly displaced division plane (Doerder et al 1975;Lynn 1977;Schäfer and Cleffmann 1982). On the other hand, in Tetrahymena whose posterior end was destroyed by cell-cell fusion, the oral primordium appears at a correct position and the nuclei are correctly guided with respect to the anteroposterior cell axis (Gaertig and Iftode 1989;Gaertig and Cole 2000). Thus, the mechanisms that generate the biased cortical localizations of Hippo pathway proteins along the anteroposterior axis remain unknown.…”

Section: Cdai Acts In the Late Hippo Circuitmentioning

confidence: 99%

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Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the CiliateTetrahymena

et al. 2018

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In a single cell, ciliates maintain a complex pattern of cortical organelles that are arranged along the anteroposterior and circumferential axes. The underlying molecular mechanisms of intracellular pattern formation in ciliates are largely unknown. Ciliates divide by tandem duplication, a process that remodels the parental cell into two daughters aligned head-to-tail. In the elo1-1 mutant of Tetrahymena thermophila, the segmentation boundary/division plane forms too close to the posterior end of the parental cell, producing a large anterior and a small posterior daughter cell, respectively. We show that ELO1 encodes a Lats/NDR kinase that marks the posterior segment of the cell cortex, where the division plane does not form in the wild-type. Elo1 acts independently of CdaI, a Hippo/ Mst kinase that marks the anterior half of the parental cell, and whose loss shifts the division plane anteriorly. We propose that, in Tetrahymena, two antagonistic Hippo circuits focus the segmentation boundary/division plane at the equatorial position, by excluding divisional morphogenesis from the cortical areas that are too close to cell ends.

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Section: Resultsmentioning

confidence: 99%

Section: Cdai Acts In the Late Hippo Circuitmentioning

confidence: 99%

Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the CiliateTetrahymena

et al. 2018

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“…These results implied the involvement of Zfr1p in conjugation junction structure stability. Importantly, an elaborate conjugation junction is required for pronuclear exchange during the conjugation stage in Tetrahymena [13]. The process of pronuclear exchange involves dramatic membrane remodeling to accompany the formation and resolution of the nuclear exchange junction during mating [14].…”

Section: Discussionmentioning

confidence: 99%

“…In Tetrahymena , a cell–cell junction is robust in order to survive the mechanical stresses experienced when two individual and highly mobile cells attempt to form a union [12]. An elaborate conjugation junction structure is also required for pronuclear exchange during the conjugation stage [13]. The pronuclear exchange is impelled by microtubule-rich baskets whose terminals are connected with the junction.…”

Section: Introductionmentioning

confidence: 99%

The Zinc Finger Protein Zfr1p Is Localized Specifically to Conjugation Junction and Required for Sexual Development in Tetrahymena thermophila

Tian

Wang

et al. 2012

PLoS ONE

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Conjugation in Tetrahymena thermophila involves a developmental program consisting of three prezygotic nuclear divisions, pronuclear exchange and fusion, and postzygotic and exconjugant stages. The conjugation junction structure appears during the initiation of conjugation development, and disappears during the exconjugant stage. Many structural and functional proteins are involved in the establishment and maintenance of the junction structure in T. thermophila. In the present study, a zinc finger protein-encoding gene ZFR1 was found to be expressed specifically during conjugation and to localize specifically to the conjugation junction region. Truncated Zfr1p localized at the plasma membrane in ordered arrays and decorated Golgi apparatus located adjacent to basal body. The N-terminal zinc finger and C-terminal hydrophobic domains of Zfr1p were found to be required for its specific conjugation junction localization. Conjugation development of ZFR1 somatic knockout cells was aborted at the pronuclear exchange and fusion conjugation stages. Furthermore, Zfr1p was found to be important for conjugation junction stability during the prezygotic nuclear division stage. Taken together, our data reveal that Zfr1p is required for the stability and integrity of the conjugation junction structure and essential for the sexual life cycle of the Tetrahymena cell.

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“…In Paramaecium and Tetrahymena, the cellular position of a nucleus is known to determine its development. 38,39 Therefore, it is possible that failure of the MIC to progress to metaphase and anaphase I is a consequence of its mislocalization.…”

Section: Meiosis Is Arrested At Diakinesis-like Metaphase I In Cyc17dmentioning

confidence: 99%

Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation inTetrahymena thermophila

et al. 2016

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Although the role of cyclins in controlling nuclear division is well established, their function in ciliate meiosis remains unknown. In ciliates, the cyclin family has undergone massive expansion which suggests that diverse cell cycle systems exist, and this warrants further investigation. A screen for cyclins in the model ciliate Tetrahymena thermophila showed that there are 34 cyclins in this organism. Only 1 cyclin, Cyc17, contains the complete cyclin core and is specifically expressed during meiosis. Deletion of CYC17 led to meiotic arrest at the diakinesis-like metaphase I stage. Expression of genes involved in DNA metabolism and chromosome organization (chromatin remodeling and basic chromosomal structure) was repressed in cyc17 knockout matings. Further investigation suggested that Cyc17 is involved in regulating spindle pole attachment, and is thus essential for chromosome segregation at meiosis. These findings suggest a simple model in which chromosome segregation is influenced by Cyc17.

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The Role of Cortical Geometry in the Nuclear Development of Tetrahymena thermophila

Cited by 10 publications

References 14 publications

Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the CiliateTetrahymena

Two Antagonistic Hippo Signaling Circuits Set the Division Plane at the Medial Position in the CiliateTetrahymena

The Zinc Finger Protein Zfr1p Is Localized Specifically to Conjugation Junction and Required for Sexual Development in Tetrahymena thermophila

Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation inTetrahymena thermophila

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