The role of current product release criteria for identification of human islet preparations suitable for clinical transplantation

Eckhard, Michael; Brandhorst, Daniel; Winter, Daniel T; Jaeger, C.; Jahr, H; Bretzel, Reinhard G.; Brendel, Mathias D.

doi:10.1016/j.transproceed.2004.06.001

Cited by 27 publications

(23 citation statements)

References 13 publications

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“…This is in accordance with the previous reports that most of the currently used methods for islet product release criteria were not able to foresee transplantation outcome (5)(6)(7)14).…”

Section: Discussionsupporting

confidence: 93%

The ADP/ATP Ratio: A Novel Predictive Assay for Quality Assessment of Isolated Pancreatic Islets

Goto

Holgersson

Kumagai‐Braesch

et al. 2006

American Journal of Transplantation

106

101

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The current standard assays for islet product release criteria are unable to predict the outcome after clinical islet transplantation. Therefore, establishment of reliable and rapid assays enabling pre-transplantation prediction of islet potency is warranted. In the present study, we have evaluated the adenosine diphosphate (ADP)/adenosine triphosphate (ATP) test, the glucose-stimulated insulin release, the loss of islets during the first 24 h in culture, and the insulin/deoxyribonucleic acid as predictive assays for the ability of isolated porcine islets to cure athymic mice with streptozotocin-induced diabetes. From the results presented, it is concluded that the measurement of the ADP/ATP ratio was the only test that correlated with transplantation outcome. In summary, we propose that the ADP/ATP assay is worthwhile as applied to human islet transplantation and seek to validate it as a rapid and potent predictor of transplant outcome.

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“…This is in accordance with the previous reports that most of the currently used methods for islet product release criteria were not able to foresee transplantation outcome (5)(6)(7)14).…”

Section: Discussionsupporting

confidence: 93%

The ADP/ATP Ratio: A Novel Predictive Assay for Quality Assessment of Isolated Pancreatic Islets

Goto

Holgersson

Kumagai‐Braesch

et al. 2006

American Journal of Transplantation

106

101

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show abstract

“…But partial thrombosis of portal vein branches (51) and, in the long-term, tissue remodeling and morphological alteration into the liver (13,52) have been described as complications of large tissue volume transplantation. In addition, in a univariate analysis, the islet purity level was directly correlated with the C-peptide value of recipients a month after transplant (12), although this was not confirmed by the multivariate analysis or other studies (12,53).…”

Section: Islet Preparation Compositionmentioning

confidence: 74%

Prediction of Clinical Outcome in Islet Allotransplantation

Bertuzzi

Ricordi

2007

Diabetes Care

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“…This requirement has not been achieved, in part because the characteristics determined by currently accepted assay methods, individually or collectively, do not correlate with transplantation outcome. [2][3][4][5][6][7][8][9] Development of meaningful assays of islet properties is challenging for several reasons 8 : (1) Many techniques suitable for cells are not practical with islets because of their three-dimensional aggregate structure. Islets cannot be usefully dissociated into dispersed cells by agitation and incubation with serine proteases such as trypsin because from 30% to 50% of the cells are lost during dissociation.…”

Section: Introductionmentioning

confidence: 99%