The Role of Cysteine Residues in Tellurite Resistance Mediated by the TehAB Determinant

Dyllick-Brenzinger, Melanie; Liu, Mingfu; Winstone, Tara L.; Taylor, Diane E.; Turner, Raymond J.

doi:10.1006/bbrc.2000.3686

Cited by 19 publications

(14 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We have also shown that the tehB gene product is a protein that is capable of binding both S-adenosylmethionine and tellurite (8,11). To date, our data suggest that the mechanism by which tehAB confers resistance to E. coli involves methylation of tellurite and subsequent efflux of this product from the cells.…”

Section: Discussionsupporting

confidence: 51%

“…The tehA gene encodes an integral membrane protein that has been shown to have efflux activity of quaternary ammonium compounds (33). The TehB protein has been shown to be capable of binding both S-adenosylmethionine and tellurite (8,11). This suggests that the resistance mechanism of the TehAB phenotype likely involves the methylation of tellurite, an activity observed in phytopathogenic pseudomonads (5), and subsequent efflux of an organotelluro compound from the cell.…”

mentioning

confidence: 99%

See 1 more Smart Citation

In Vivo ³¹ P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

Lohmeier-Vogel

Ung

Turner

2004

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Here we compare the physiological state of Escherichia coli exposed to tellurite or selenite by using the noninvasive technique of phosphorus-31 nuclear magnetic resonance (NMR) spectroscopy. We studied glucose-fed Escherichia coli HB101 cells containing either a normal pUC8 plasmid with no tellurite resistance determinants present or the pTWT100 plasmid which contains the resistance determinants tehAB. No differences could be observed in intracellular ATP levels, the presence or absence of a transmembrane pH gradient, or the levels of phosphorylated glycolytic intermediates when resistant cells were studied by 31 P NMR in the presence or absence of tellurite. In the sensitive strain, we observed that the transmembrane pH gradient was dissipated and intracellular ATP levels were rapidly depleted upon exposure to tellurite. Only the level of phosphorylated glycolytic intermediates remained the same as observed with resistant cells. Upon exposure to selenite, no differences could be observed by 31 P NMR between resistant and sensitive strains, suggesting that the routes for selenite and tellurite reduction within the cells differ significantly, since only tellurite is able to collapse the transmembrane pH gradient and lower ATP levels in sensitive cells. The presence of the resistance determinant tehAB, by an as yet unidentified detoxification event, protects the cells from uncoupling by tellurite.

show abstract

Section: Discussionsupporting

confidence: 51%

mentioning

confidence: 99%

In Vivo ³¹ P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

Lohmeier-Vogel

Ung

Turner

2004

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…It is a dimer that can bind both of these compounds in mediating resistance to tellurite. Tellurite generates oxidative stress and may replace sulfur in various proteins, rendering them nonfunctional (8,9,18,20). In the present study, in addition to cysK, tehA, and tehB, the genes encoding proteins such as AhpF, Dps, KatG, SoxS, and Bfr, which respond to oxidative stress, were upregulated.…”

supporting

confidence: 48%

DNA Microarray Analysis of the Expression Profile of Escherichia coli in Response to Treatment with 4,5-Dihydroxy-2-Cyclopenten-1-One

2002

View full text Add to dashboard Cite

We carried out DNA microarray-based global transcript profiling of Escherichia coli in response to 4,5-dihydroxy-2-cyclopenten-1-one to explore the manifestation of its antibacterial activity. We show that it has widespread effects in E. coli affecting genes encoding proteins involved in cell metabolism and membrane synthesis and functions. Genes belonging to the regulon involved in synthesis of Cys are upregulated. In addition, rpoS and RpoS-regulated genes responding to various stresses and a number of genes responding to oxidative stress are upregulated.A number of antibiotics are available against a variety of bacteria; however, in recent years, the emergence of multipledrug-resistant bacteria has been a primary concern. This has provided an incentive to search for newer and more effective antibacterial compounds. Previously we described one such compound, 4,5-dihydroxy-2-cyclopenten-1-one (DHCP), having antibacterial activity against a variety of gram-negative and -positive bacteria, such as Escherichia coli and Salmonella, Bacillus, and Staphylococcus spp., etc. DHCP is prepared by the heat treatment of uronic acid or its derivatives (12). It is also produced from roasted or parched vegetables, fruits, cereals, mushrooms, sea algae, cortex, or cartilage. It has been shown elsewhere to have potential applications as a therapeutic or preventive agent against cancer and also as an antibacterial agent in antiseptics, dentifrices, cosmetics, and bathing agents (12). We isolated a multicopy suppressor of DHCP toxicity from an E. coli genomic library. The gene encoding this suppressor was designated dep, and the putative protein encoded by this gene was designated Dep. The Dep protein showed high homology to known efflux proteins conferring resistance to a number of antibiotics including chloramphenicol, bicyclomycin, and tetracycline. However, it did not confer cross-resistance to any of the antibiotics tested (15). The exact mechanism of action of DHCP is not known.In the present study, we analyzed the global transcriptional pattern of E. coli in response to DHCP by DNA microarraybased technology to explore the manifestation of the antibacterial activity of DHCP. We show that DHCP has widespread effects in E. coli affecting genes encoding proteins involved in cell metabolism and membrane synthesis and functions.DNA microarray-based global transcription profiling of E. coli in response to DHCP treatment. Previously we showed that the growth of E. coli strain JM83 [F Ϫ ara⌬(lac-proAB) rpsL(Str r )] (22) was impaired in the presence of DHCP (250 M) after 3 h of incubation and that cells stopped growing after 5 h (15). Our main objective in the present study was to identify all of the E. coli open reading frames that exhibited a significant increase or decrease in mRNA abundance caused by the DHCP treatment. The cells were grown in the presence of 250 M DHCP as described previously (15). In brief, cells grown overnight in Luria-Bertani medium were diluted into fresh Luria-Bertani medium. After the growth reached 50...

show abstract

“…Cysteine synthase proteins and molecules containing cysteine, including glutathione, have previously been shown to be involved in tellurite resistance in E. coli (15,49,59,60). Tellurite resistance in S. aureus is uncharacterized, and furthermore, little is known about cysteine biosynthesis and its importance in this bacterium.…”

Section: Resultsmentioning

confidence: 99%

“…Cysteine residues are known to be important in the function of many metal-binding proteins (22). The E. coli tellurite resistance determinants TehA and TehB each contain three cysteine residues, and replacement of these cysteines with alanine residues by site-directed mutagenesis leads to a decrease in tellurite resistance (15). In addition, the thiol redox enzymes (glutathione reductase and thioredoxin reductase) and their metabolites (glutathione, glutaredoxin, and thioredoxin), which all contain cysteine residues, have been shown to be involved in tellurite resistance (59,60).…”

Section: Discussionmentioning

confidence: 99%

Role of a Cysteine Synthase in Staphylococcus aureus

et al. 2004

View full text Add to dashboard Cite

The gram-positive human pathogen Staphylococcus aureus is often isolated with media containing potassium tellurite, to which it has a higher level of resistance than Escherichia coli. The S. aureus cysM gene was isolated in a screen for genes that would increase the level of tellurite resistance of E. coli DH5␣. The protein encoded by S. aureus cysM is sequentially and functionally homologous to the O-acetylserine (thiol)-lyase B family of cysteine synthase proteins. An S. aureus cysM knockout mutant grows poorly in cysteine-limiting conditions, and analysis of the thiol content in cell extracts showed that the cysM mutant produced significantly less cysteine than wild-type S. aureus SH1000. S. aureus SH1000 cannot use sulfate, sulfite, or sulfonates as the source of sulfur in cysteine biosynthesis, which is explained by the absence of genes required for the uptake and reduction of these compounds in the S. aureus genome. S. aureus SH1000, however, can utilize thiosulfate, sulfide, or glutathione as the sole source of sulfur. Mutation of cysM caused increased sensitivity of S. aureus to tellurite, hydrogen peroxide, acid, and diamide and also significantly reduced the ability of S. aureus to recover from starvation in amino acid-or phosphate-limiting conditions, indicating a role for cysteine in the S. aureus stress response and survival mechanisms.

show abstract

The Role of Cysteine Residues in Tellurite Resistance Mediated by the TehAB Determinant

Cited by 19 publications

References 27 publications

In Vivo ³¹ P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

In Vivo ³¹ P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

DNA Microarray Analysis of the Expression Profile of Escherichia coli in Response to Treatment with 4,5-Dihydroxy-2-Cyclopenten-1-One

Role of a Cysteine Synthase in Staphylococcus aureus

Contact Info

Product

Resources

About

The Role of Cysteine Residues in Tellurite Resistance Mediated by the TehAB Determinant

Cited by 19 publications

References 27 publications

In Vivo 31 P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

In Vivo 31 P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

DNA Microarray Analysis of the Expression Profile of Escherichia coli in Response to Treatment with 4,5-Dihydroxy-2-Cyclopenten-1-One

Role of a Cysteine Synthase in Staphylococcus aureus

Contact Info

Product

Resources

About

In Vivo ³¹ P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli

In Vivo ³¹ P Nuclear Magnetic Resonance Investigation of Tellurite Toxicity in Escherichia coli