The role of decapping proteins in the miRNA accumulation in<i>Arabidopsis thaliana</i>

Motomura, Kazuki; Le, Quy Tn; Kumakura, Naoyoshi; Fukaya, Takashi; Takeda, Atsushi; Watanabe, Yoshihisa

doi:10.4161/rna.19877

Cited by 30 publications

(28 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…DCP1 and VCS may be required for the degradation of some miRNA targets, suggesting a possible role in target destabilization; however, the decapping complex also has a broader role in the destabilization of many nontarget mRNAs (Xu et al, 2006;Goeres et al, 2007;Motomura et al, 2012). A relationship between decapping and slicing has not yet been established; therefore, a role for VCS in slicing-independent target destabilization may be an alternative explanation for target mRNA accumulation in vcs mutants.…”

Section: Decapping Complexmentioning

confidence: 99%

“…A relationship between decapping and slicing has not yet been established; therefore, a role for VCS in slicing-independent target destabilization may be an alternative explanation for target mRNA accumulation in vcs mutants. Alternatively, secondary effects of decapping mutants on miRNA levels may contribute to the observed target upregulation (Motomura et al, 2012). VCS also mediates translational repression as some targets are upregulated only at the protein level in vcs mutants (Brodersen et al, 2008).…”

Section: Decapping Complexmentioning

confidence: 99%

See 1 more Smart Citation

Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs

2013

View full text Add to dashboard Cite

(K.R.).MicroRNAs (miRNAs) are small RNAs that control gene expression through silencing of target mRNAs. Mature miRNAs are processed from primary miRNA transcripts by the endonuclease activity of the DICER-LIKE1 (DCL1) protein complex. Mechanisms exist that allow the DCL1 complex to precisely excise the miRNA from its precursor. Our understanding of miRNA biogenesis, particularly its intersection with transcription and other aspects of RNA metabolism such as splicing, is still evolving. Mature miRNAs are incorporated into an ARGONAUTE (AGO) effector complex competent for target gene silencing but are also subjected to turnover through a degradation mechanism that is beginning to be understood. The mechanisms of miRNA target silencing in plants are no longer limited to AGO-catalyzed slicing, and the contribution of translational inhibition is increasingly appreciated. Here, we review the mechanisms underlying the biogenesis, turnover, and activities of plant miRNAs.

show abstract

Section: Decapping Complexmentioning

confidence: 99%

Section: Decapping Complexmentioning

confidence: 99%

Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs

2013

View full text Add to dashboard Cite

show abstract

“…Total RNA was extracted from Arabidopsis inflorescences and the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa) was used to synthesize the cDNA according to the manufacturer's protocol. qPCR was performed with the StepOnePlus Real Time PCR System (Applied Biosystems; Life Technologies) using a KAPA SYBR Fast qPCR kit (KAPA BIOSYSTEMS) and reaction conditions as previously described (Motomura et al 2012). ACTIN2 was used as the internal control gene.…”

Section: Quantitative Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

Recovery of dicer-like 1-late flowering phenotype by miR172 expressed by the noncanonical DCL4-dependent biogenesis pathway

Tsuzuki

Takeda

Watanabe

2014

RNA

Self Cite

View full text Add to dashboard Cite

MicroRNAs (miRNAs) act as down-regulators of gene expression, and play a dominant role in eukaryote development. In Arabidopsis thaliana, DICER-LIKE 1 (DCL1) is the main processor in miRNA biogenesis, and dcl1 mutants show various developmental defects at the early stage of embryogenesis or at gamete formation. However, miRNAs responsible for the respective developmental stages of the dcl1 defects have not been identified. Here, we developed a DCL1-independent miRNA expression system using the unique DCL4-dependent miRNA, miR839. By replacing the mature sequence in the miR839 precursor sequence with that of miR172, one of the most widely conserved miRNAs in angiosperms, we succeeded in expressing miR172 from a chimeric miR839 precursor in dcl1-7 plants and observed the repression of miR172 target gene expression. In parallel, the DCL4-dependent miR172 expression rescued the late flowering phenotype of dcl1-7 by acceleration of flowering. We established the DCL1-independent miRNA expression system, and revealed that the reduction of miR172 expression is responsible for the dcl1-7 late flowering phenotype.

show abstract

“…However, the causal relationship between translational repression and decay of target RNAs has not been established in plants. Mutations in VCS and decapping1 (DCP1; a P-body component required for mRNA decapping) reduce the levels of some target transcripts, indicating the presence of slicer-independent degradation of targets [158]. However, the upregulation of target transcripts in vcp and dcp1 may attribute to the decreased miRNA levels [158].…”

Section: Mirna-mediated Translational Inhibitionmentioning

confidence: 99%

microRNA biogenesis, degradation and activity in plants

Xie

Zhang

2014

Cell. Mol. Life Sci.

115

View full text Add to dashboard Cite

microRNAs (miRNAs) are important regulators of gene expression. After excised from primary miRNA transcript by dicer-like1 (DCL1, an RNAse III enzyme), miRNAs bind and guide their effector protein named argonaute 1 (AGO1) to silence the expression of target RNAs containing their complementary sequences in plants. miRNA levels and activities are tightly controlled to ensure their functions in various biological processes such as development, metabolism and responses to abiotic and biotic stresses. Studies have identified many factors that involve in miRNA accumulation and activities. Characterization of these factors in turn greatly improves our understanding of the processes related to miRNAs. Here, we review recent progress of mechanisms underlying miRNA expression and functions in plants.

show abstract

The role of decapping proteins in the miRNA accumulation inArabidopsis thaliana

Cited by 30 publications

References 55 publications

Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs

Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs

Recovery of dicer-like 1-late flowering phenotype by miR172 expressed by the noncanonical DCL4-dependent biogenesis pathway

microRNA biogenesis, degradation and activity in plants

Contact Info

Product

Resources

About