The role of delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh) in the Pacific white shrimp (Litopenaeus vannamei) during biotic and abiotic stress

Liang, QingJian; Wu, XuJian; Pan, Yang; Kong, Jinrong; Wei, Wei; Qiao, Xue-Li; Liu, Yuan; Wang, Weina

doi:10.1016/j.aquatox.2018.12.016

Cited by 26 publications

(8 citation statements)

References 44 publications

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“…In this study, proline content in tomato leaves was increased in both inoculated and non-inoculated tomato under salt stress ( Figure 2 B). The common pathway for proline biosynthesis derives from glutamate [ 95 , 96 ]. Several predictive genes related to proline synthesis via glutamate were found in genome of D. abyssi MT1.1 T including delta-1-pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate reductase, gamma-glutamyl phosphate reductase, and glutamate 5-kinase.…”

Section: Discussionmentioning

confidence: 99%

Plant Beneficial Deep-Sea Actinobacterium, Dermacoccus abyssi MT1.1T Promote Growth of Tomato (Solanum lycopersicum) under Salinity Stress

Rangseekaew

Barros-Rodríguez

Pathom-aree

et al. 2022

Biology

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Salt stress is a serious agricultural problem threatens plant growth and development resulted in productivity loss and global food security concerns. Salt tolerant plant growth promoting actinobacteria, especially deep-sea actinobacteria are an alternative strategy to mitigate deleterious effects of salt stress. In this study, we aimed to investigate the potential of deep-sea Dermacoccus abyssi MT1.1T to mitigate salt stress in tomato seedlings and identified genes related to plant growth promotion and salt stress mitigation. D. abyssi MT1.1T exhibited plant growth promoting traits namely indole-3-acetic acid (IAA) and siderophore production and phosphate solubilization under 0, 150, 300, and 450 mM NaCl in vitro. Inoculation of D. abyssi MT1.1T improved tomato seedlings growth in terms of shoot length and dry weight compared with non-inoculated seedlings under 150 mM NaCl. In addition, increased total soluble sugar and total chlorophyll content and decreased hydrogen peroxide content were observed in tomato inoculated with D. abyssi MT1.1T. These results suggested that this strain mitigated salt stress in tomatoes via osmoregulation by accumulation of soluble sugars and H2O2 scavenging activity. Genome analysis data supported plant growth promoting and salt stress mitigation potential of D. abyssi MT1.1T. Survival and colonization of D. abyssi MT1.1T were observed in roots of inoculated tomato seedlings. Biosafety testing on D. abyssi MT1.1T and in silico analysis of its whole genome sequence revealed no evidence of its pathogenicity. Our results demonstrate the potential of deep-sea D. abyssi MT1.1T to mitigate salt stress in tomato seedlings and as a candidate of eco-friendly bio-inoculants for sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Plant Beneficial Deep-Sea Actinobacterium, Dermacoccus abyssi MT1.1T Promote Growth of Tomato (Solanum lycopersicum) under Salinity Stress

Rangseekaew

Barros-Rodríguez

Pathom-aree

et al. 2022

Biology

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“…This result indicated that P5CS may have several subtypes or it may perform different functions in different species. Although there is no research about P5CS in crustaceans, there are some studies about P5C dehydrogenase ( P5CDh ) in crustaceans, especially in L. vannamei [ 63 ]. Unlike P5CS , which catalyzes the reduction of glutamic acid (Glu) to glutamic-γ-semialdehyde and pyrroline-5-carboxylate, P5CDh converts the latter two to the former [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…Different from the expression of P5CDh in L. vannamei in response to environmental changes, the expression of P5CS in M. nipponense increased gradually after reaching the lowest level 6 h after hypoxia. Previous studies have found that LvP5CDh plays a key role in immune defense and antioxidant response [ 63 ]. These results suggest that P5CS and P5CDh may act as a pair of antagonistic genes to regulate biological homeostasis in response to environmental changes, including changes in oxygen, pH, and salinity.…”

Section: Discussionmentioning

confidence: 99%

Integrated Metabolomics and Transcriptomic Analysis of Hepatopancreas in Different Living Status Macrobrachium nipponense in Response to Hypoxia

Zhang

Qiao

et al. 2021

Antioxidants

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As the basic element of aerobic animal life, oxygen participates in most physiological activities of animals. Hypoxia stress is often the subject of aquatic animal research. Macrobrachium nipponense, an economically important aquatic animal in southern China, has been affected by hypoxia for many years and this has resulted in a large amount of economic loss due to its sensitivity to hypoxia; Metabolism and transcriptome data were combined in the analysis of the hepatopancreas of M. nipponense in different physiological states under hypoxia; A total of 108, 86, and 48 differentially expressed metabolites (DEMs) were found in three different comparisons (survived, moribund, and dead shrimps), respectively. Thirty-two common DEMs were found by comparing the different physiological states of M. nipponense with the control group in response to hypoxia. Twelve hypoxia-related genes were identified by screening and analyzing common DEMs. GTP phosphoenolpyruvate carboxykinase (PEPCK) was the only differentially expressed gene that ranked highly in transcriptome analysis combined with metabolome analysis. PEPCK ranked highly both in transcriptome analysis and in combination with metabolism analysis; therefore, it was considered to have an important role in hypoxic response. This manuscript fills the one-sidedness of the gap in hypoxia transcriptome analysis and reversely deduces several new genes related to hypoxia from metabolites. This study contributes to the clarification of the molecular process associated with M. nipponense under hypoxic stress.

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“…Total RNA was extracted using Trizol (Invitrogen) according to the manufacturer's recommendations and previous description (Liang et al, 2019c). The concentration and quality of the RNA were determined by the 260/280-nm spectrophotometer (NanoDrop Technologies, Montchanin, DE) and 1.5% agarose gel electrophoresis.…”

Section: Quantitative Real-time Polymerase Chain Reaction (Rt-qpcr)mentioning

confidence: 99%

miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature Stress

Liang

Dong

et al. 2021

Front. Cell Dev. Biol.

Self Cite

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MicroRNAs (miRNAs) play key roles in many physiologic and pathologic processes, including autophagy. Autophagy is cellular in an emergency response mechanism of environment stress, but their complex molecular regulatory mechanism under low-temperature stress is largely unknown in shrimp, especially miRNA-mediated regulation of autophagy in low-temperature tolerance. In this article, a shrimp PvTOR and miRNA pva-miR-151 cooperation in response to low-temperature stress has been reported. Pva-miR-151 showed expression patterns opposite to target PvTOR under low-temperature stress. The pva-miR-151 targets the 3′-UTR region of PvTOR, regulate the formation of autophagosome, which contribute to the degradation and recycling of damaged organelles. In addition, the low-temperature tolerance was correlated positively with autophagy in shrimp. Silenced pva-miR-151 increased sensitivity to low-temperature stress, whereas overexpression pva-miR-151 decreased the expression of PvTOR and p-TOR and increased tolerance to low-temperature stress by improving the formation of autophagosome and total hemocyte count. In addition, the TOR activator 3BDO can partially rescue autophagy induced by overexpression of pva-miR-151; these results indicate that miR-151 was necessary for the low-temperature tolerance in shrimp. Taken together, we provide a novel strategy and mechanism for shrimp breeding to improve shrimp low-temperature tolerance.

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The role of delta-1-pyrroline-5-carboxylate dehydrogenase (P5CDh) in the Pacific white shrimp (Litopenaeus vannamei) during biotic and abiotic stress

Cited by 26 publications

References 44 publications

Plant Beneficial Deep-Sea Actinobacterium, Dermacoccus abyssi MT1.1T Promote Growth of Tomato (Solanum lycopersicum) under Salinity Stress

Plant Beneficial Deep-Sea Actinobacterium, Dermacoccus abyssi MT1.1T Promote Growth of Tomato (Solanum lycopersicum) under Salinity Stress

Integrated Metabolomics and Transcriptomic Analysis of Hepatopancreas in Different Living Status Macrobrachium nipponense in Response to Hypoxia

miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature Stress

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